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Phosphorylcholine

Cat No.:V33980 Purity: ≥98%
Phosphorylcholine chloride (Phosphocholine chloride) is a component of lecithin that forms the outer structure of cell membranes and has good blood compatibility.
Phosphorylcholine
Phosphorylcholine Chemical Structure CAS No.: 107-73-3
Product category: New2
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
500mg
Official Supplier of:
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Product Description
Phosphorylcholine chloride (Phosphocholine chloride) is a component of lecithin that forms the outer structure of cell membranes and has good blood compatibility.
Biological Activity I Assay Protocols (From Reference)
ln Vitro
Phosphorylcholine materials, which have a molecular replica of the main zwitterionic phospholipid headgroup present in the cell lipid membrane, are bioinspired polymers that resemble the extracellular surface of red blood cells. The PC-polymer's phosphorylcholine side chain includes the phosphate moiety found in phosphatidylcholine and phosphatidylserine, the latter of which is a membrane lipid component involved in biomineralization and capable of binding calcium[1].
References

[1]. The effect of cationically-modified phosphorylcholine polymers on human osteoblasts in vitro and their effect on bone formation in vivo. J Mater Sci Mater Med. 2017 Aug 17;28(9):144.

Additional Infomation
Phosphorylcholine chloride is an organochloride composed of phosphoric acid cations and chloride ions. It contains phosphoric acid choline and chloride ions. Calcium and magnesium salts can be used to treat hepatobiliary dysfunction.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C5H15CLNO4P
Molecular Weight
219.60400
Exact Mass
219.042
CAS #
107-73-3
PubChem CID
7886
Appearance
Colorless to light yellow solid-liquid Mixture
Hydrogen Bond Donor Count
2
Hydrogen Bond Acceptor Count
5
Rotatable Bond Count
4
Heavy Atom Count
12
Complexity
158
Defined Atom Stereocenter Count
0
SMILES
O=P(OCC[N+](C)(C)C)(O)O.[Cl-]
InChi Key
PYJNAPOPMIJKJZ-UHFFFAOYSA-N
InChi Code
InChI=1S/C5H14NO4P.ClH/c1-6(2,3)4-5-10-11(7,8)9;/h4-5H2,1-3H3,(H-,7,8,9);1H
Chemical Name
trimethyl(2-phosphonooxyethyl)azanium;chloride
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
H2O : ~250 mg/mL (~1138.43 mM)
Solubility (In Vivo)
Solubility in Formulation 1: 100 mg/mL (455.37 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.

 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 4.5537 mL 22.7687 mL 45.5373 mL
5 mM 0.9107 mL 4.5537 mL 9.1075 mL
10 mM 0.4554 mL 2.2769 mL 4.5537 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

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An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
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  • Enter 10 in the Concentration box and choose the correct unit (mM)
  • Enter 5 in the Volume box and choose the correct unit (mL)
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  • The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
  • Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
  • Enter 25 into the Concentration (End) box and select the correct unit (mM)
  • Enter 25 into the Volume (End) box and choose the correct unit (mL)
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  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:
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Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
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Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

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In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

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