| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
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| ln Vitro |
The 5-HT3 receptor selective agonist phenylbiguanide (1-Phenylbiguanide) has pEC50 values of 5.57, 4.07, and 4.47 for r5-HT3A(b), h5-HT3A, and m5-HT3A(b), respectively [1]. It was investigated how phenylbiguanide (1-phenylbiguanide hydrochloride) encouraged the proliferation of the HT29 cell line. After 48 hours of incubation, phenolbiguanide caused dose-dependent proliferation of HT29 cells. At 12.5 μM (5HT), the maximum proliferation was observed (P≤0.01). Significant stimulation of cell growth was seen at doses of 3.125 μM (P≤0.05) and 6.25 μM (P≤0.01) for benformin [2].
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|---|---|
| ln Vivo |
The right atrium of the heart was perfused with phenformin (PBG), a medication that is known to activate cardiorespiratory afferent C fibers, in anesthetized mice, and the expression of c-Fos was mapped in particular areas of the central nervous system. Respiratory depression and marked bradycardia are among the reflex cardiorespiratory responses elicited by intraatrial PBG injection [3].
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| References |
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| Additional Infomation |
Phenyl biguanide is a member of the class of biguanides that is biguanide in which one of the terminal nitrogen atoms is substituted by a phenyl group. It has a role as a central nervous system drug. It is functionally related to a biguanide.
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| Molecular Formula |
C8H11N5
|
|---|---|
| Molecular Weight |
177.2064
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| Exact Mass |
177.101
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| CAS # |
102-02-3
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| Related CAS # |
55-57-2 (mono-hydrochloride)
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| PubChem CID |
4780
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| Appearance |
White to off-white solid powder
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| Density |
1.33g/cm3
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| Boiling Point |
388.4ºC at 760mmHg
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| Melting Point |
135-142 °C(lit.)
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| Flash Point |
188.7ºC
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| Vapour Pressure |
3.08E-06mmHg at 25°C
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| Index of Refraction |
1.656
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| LogP |
1.88
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
13
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| Complexity |
211
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
CUQCMXFWIMOWRP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H11N5/c9-7(10)13-8(11)12-6-4-2-1-3-5-6/h1-5H,(H6,9,10,11,12,13)
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| Chemical Name |
1-(diaminomethylidene)-2-phenylguanidine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~150 mg/mL (~846.45 mM)
H2O : ≥ 25 mg/mL (~141.08 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (14.11 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.11 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (14.11 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.6430 mL | 28.2151 mL | 56.4302 mL | |
| 5 mM | 1.1286 mL | 5.6430 mL | 11.2860 mL | |
| 10 mM | 0.5643 mL | 2.8215 mL | 5.6430 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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