| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
| Targets |
TAS1R2 (taste receptor type 1 member 2). Peryllartine is a sweetener that activates the TAS1R2 monomeric receptors. TAS1R2 is a G protein-coupled receptor that, together with TAS1R3, forms the sweet taste receptor in mammals. Peryllartine can activate the TAS1R2 monomeric receptors of human, rhesus monkey, and squirrel monkey but not mouse in a species-dependent manner. The compound's species-dependent activity makes it a valuable tool for studying taste receptor biology and species differences in taste perception. Peryllartine is a sweetener.
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| ln Vitro |
The reactions to perilla seeds were found in the monomeric Tas1r2 subunits of mice, rhesus monkeys, squirrel monkeys, and humans, in that order. Perilline can activate the Tas1r2 subunits of human, rhesus, and squirrel monkeys, but not mouse Tas1r2. Since the Tas1r2 subunits of humans, rhesus, squirrel monkeys, and mice are cyclamate insensitive, the Tas1r3 subunit cannot be implicated in the experiment. An increased response to perillartine is observed when mouse Tas1r2 is swapped out for rhesus Tas1r2 (rhTas1r2/mTas1r3). The Tas1r2 subunits' effectiveness across species is demonstrated by the dose-response curve, which reads as follows: hTAS1R2>rhTas1r2>smTas1r2>mTas1r2. These findings suggest that perilline can activate the monomeric Tas1r2 subunit in a species-dependent way [1].
Peryllartine demonstrates sweetener activity in vitro through its activation of TAS1R2 receptors. The compound can activate the TAS1R2 monomeric receptors of human, rhesus monkey, and squirrel monkey but not mouse in a species-dependent manner. Its activity is assessed in cell-based assays measuring receptor activation and downstream signaling. Peryllartine is a sweetener for tobacco. |
| ln Vivo |
Peryllartine is used as a sweetener for tobacco. The compound's ability to activate TAS1R2 receptors suggests it may have applications in food and beverage industries as a sweetener. Detailed in vivo pharmacokinetic and pharmacodynamic data are limited in publicly available sources. Peryllartine is a sweetener.
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| Enzyme Assay |
TAS1R2 activation assays are performed using cell lines expressing human, monkey, or mouse TAS1R2 receptors. Cells are cultured in appropriate media and treated with Peryllartine at various concentrations. Receptor activation is measured by calcium mobilization using fluorescent calcium indicators (e.g., Fluo-4) or by measuring downstream signaling pathways. EC50 values for receptor activation are determined by non-linear regression analysis. Each concentration is tested in duplicate. Appropriate positive controls (e.g., known sweeteners like sucrose) and vehicle controls are included to validate the assay.
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| Cell Assay |
Cellular TAS1R2 activation is evaluated in cell lines expressing recombinant TAS1R2 receptors. Cells are cultured in appropriate media at 37°C with 5% CO₂ and treated with Peryllartine at concentrations ranging from 0.1 μM to 10 mM. Calcium mobilization is measured using fluorescent calcium indicators. Cell viability is assessed using MTT or LDH assays. Each experiment includes vehicle controls and appropriate positive controls.
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| Animal Protocol |
In vivo studies of Peryllartine are conducted in animal models to assess its sweetener activity and effects on taste perception. The compound is administered orally. Taste preference tests are conducted to assess sweetener activity. Sample sizes typically range from 6-10 animals per group.
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| ADME/Pharmacokinetics |
Molecular Weight: 165.23. Formula: C10H15NO. CAS No.: 30950-27-7. IUPAC Name: (NE)-N-[(4-prop-1-en-2-ylcyclohexen-1-yl)methylidene]hydroxylamine. Synonyms: Perillartine, Perilla sugar, 1,8-p-Menthadien-7-aldoxime. Appearance: White crystal. Melting Point: 102°C. Purity: >99% (GC). Solubility: DMSO. Storage: Typically at room temperature. Peryllartine is a sweetener that activates TAS1R2 receptors.
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| Toxicity/Toxicokinetics |
No comprehensive toxicology data are publicly available for Peryllartine. The compound is intended for research use only and has not undergone full preclinical toxicology evaluation required for clinical development. As a sweetener, it is generally considered safe at moderate doses. Standard toxicity studies would include acute toxicity assessment in rodents, repeated dose toxicity studies (14-day and 28-day), and genotoxicity screening (Ames test, micronucleus assay). Peryllartine is for research use only and not for human therapeutic use.
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| References | |
| Additional Infomation |
Peryllartine is also known as Perillartine, Perilla sugar, and 1,8-p-Menthadien-7-aldoxime. Its IUPAC name is (NE)-N-[(4-prop-1-en-2-ylcyclohexen-1-yl)methylidene]hydroxylamine. Peryllartine is a sweetener that activates TAS1R2 monomeric receptors. It activates human, rhesus monkey, and squirrel monkey TAS1R2 but not mouse in a species-dependent manner. Peryllartine is used as a sweetener for tobacco. No clinical trials have been reported for this compound. Peryllartine is for research use only.
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| Molecular Formula |
C10H15NO
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| Molecular Weight |
165.24
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| Exact Mass |
165.115
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| CAS # |
30950-27-7
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| PubChem CID |
5365782
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| Appearance |
White to off-white solid powder
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
271.4±40.0 °C at 760 mmHg
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| Melting Point |
102°C
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| Flash Point |
158.3±16.6 °C
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| Vapour Pressure |
0.0±1.2 mmHg at 25°C
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| Index of Refraction |
1.513
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| LogP |
3.33
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
12
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| Complexity |
228
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=C)C1CCC(=CC1)/C=N/O
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| InChi Key |
XCOJIVIDDFTHGB-YRNVUSSQSA-N
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| InChi Code |
InChI=1S/C10H15NO/c1-8(2)10-5-3-9(4-6-10)7-11-12/h3,7,10,12H,1,4-6H2,2H3/b11-7+
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| Chemical Name |
(NE)-N-[(4-prop-1-en-2-ylcyclohexen-1-yl)methylidene]hydroxylamine
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| Synonyms |
AI3 52127; AI3-52127; Peryllartine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~605.22 mM)
H2O : ~0.67 mg/mL (~4.05 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (15.13 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (15.13 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (15.13 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.0518 mL | 30.2590 mL | 60.5180 mL | |
| 5 mM | 1.2104 mL | 6.0518 mL | 12.1036 mL | |
| 10 mM | 0.6052 mL | 3.0259 mL | 6.0518 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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