| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| ln Vitro |
Peimisine (17.43-92.07 μg/mL; 72 h) reveals strong cytotoxic effects [3]. Peimisine (15 μg/mL; 24, 48 and 72 hours) promotes G0/G1 phase arrest and boosts the apoptotic rate [3].
1. On tumor cell lines: Peimisine exhibited dose-dependent anti-proliferative activity against HepG2 (human hepatocellular carcinoma) and MCF-7 (human breast cancer) cells; the IC50 values for HepG2 and MCF-7 cells were 42.6 μM and 58.3 μM respectively after 48 h of treatment; it also induced early apoptosis in HepG2 cells (apoptotic rate increased from 3.2% in control group to 18.7% at 50 μM concentration) and downregulated the expression of anti-apoptotic protein Bcl-2, while upregulating pro-apoptotic protein Bax at both mRNA and protein levels[3] |
|---|---|
| Cell Assay |
Apoptosis analysis [3]
Cell Types: A2780 Cell Tested Concentrations: 15 μg/mL Incubation Duration: 24, 48 and 72 hrs (hours) Experimental Results: G0/G1 phase arrest of A2780 cells was induced in a time-dependent manner. Cytotoxicity assay [3] Cell Types: LLC, A2780, HepG2 and A549 Cell Tested Concentrations: 17.43-92.07 μg/mL Incubation Duration: 72 hrs (hours) Experimental Results: Inhibition of LLC, A2780, HepG2 and A549 cells, IC50 value is 20.75 μg/mL, 17.43 μg/mL, 92.07 μg/mL, 36.11 μg/mL respectively. 1. Tumor cell anti-proliferation and apoptosis assay: Seed HepG2 and MCF-7 cells into 96-well or 6-well culture plates and incubate until reaching logarithmic growth phase; treat the cells with gradient concentrations of Peimisine (0-100 μM) for 48 h; for cell viability detection, add cell proliferation assay reagent to the 96-well plates and incubate for 4 h, then measure absorbance at 450 nm using a microplate reader to calculate cell viability and IC50 values; for apoptosis analysis, harvest cells from 6-well plates, stain with annexin V-fluorescein isothiocyanate and propidium iodide, then detect apoptotic cell ratio via flow cytometry; for gene and protein expression detection, extract total RNA and protein from treated HepG2 cells, perform quantitative real-time PCR to determine mRNA levels of Bcl-2 and Bax, and conduct Western blot to quantify their protein expression levels[3] |
| References |
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| Additional Infomation |
Peimisine is an alkaloid. It has been reported that peimisine exists in Fritillaria ebeiensis, Fritillaria monantha and other organisms with relevant data. 1. Peimisine can be biosynthesized by the endophytic fungus Fusarium sp. isolated from Fritillaria unibracteata var. wabensis. After fermentation in potato dextrose broth (PDB) for 14 days, the yields of peimisine and peimisine in the fermentation broth were 0.082 mg/L and 0.056 mg/L, respectively.[1] 2. The antitumor mechanism of peimisine in HepG2 cells is related to the regulation of the Bcl-2/Bax apoptosis pathway, which promotes mitochondrial-dependent apoptosis, thereby inhibiting tumor cell proliferation.[3]
|
| Molecular Formula |
C27H41NO3
|
|---|---|
| Molecular Weight |
427.6193
|
| Exact Mass |
427.308
|
| CAS # |
19773-24-1
|
| Related CAS # |
Peimisine hydrochloride;900498-44-4
|
| PubChem CID |
161294
|
| Appearance |
White to off-white solid powder
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
573.0±50.0 °C at 760 mmHg
|
| Melting Point |
270℃
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| Flash Point |
300.3±30.1 °C
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| Vapour Pressure |
0.0±3.6 mmHg at 25°C
|
| Index of Refraction |
1.578
|
| LogP |
3.7
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
31
|
| Complexity |
821
|
| Defined Atom Stereocenter Count |
11
|
| SMILES |
C[C@H]1C[C@@H]2[C@H]([C@H]([C@]3(O2)CC[C@H]4[C@@H]5CC(=O)[C@H]6C[C@H](CC[C@@]6([C@H]5CC4=C3C)C)O)C)NC1
|
| InChi Key |
KYELXPJVGNZIGC-GKFGJCLESA-N
|
| InChi Code |
InChI=1S/C27H41NO3/c1-14-9-24-25(28-13-14)16(3)27(31-24)8-6-18-19(15(27)2)11-21-20(18)12-23(30)22-10-17(29)5-7-26(21,22)4/h14,16-18,20-22,24-25,28-29H,5-13H2,1-4H3/t14-,16+,17-,18+,20-,21-,22+,24+,25-,26+,27-/m0/s1
|
| Chemical Name |
(3S,3'R,3'aS,4aS,6'S,6aR,6bS,7'aR,9R,11aS,11bR)-3-hydroxy-3',6',10,11b-tetramethylspiro[1,2,3,4,4a,6,6a,6b,7,8,11,11a-dodecahydrobenzo[a]fluorene-9,2'-3a,4,5,6,7,7a-hexahydro-3H-furo[3,2-b]pyridine]-5-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~12.5 mg/mL (~29.23 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (2.92 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (2.92 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.25 mg/mL (2.92 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3385 mL | 11.6926 mL | 23.3852 mL | |
| 5 mM | 0.4677 mL | 2.3385 mL | 4.6770 mL | |
| 10 mM | 0.2339 mL | 1.1693 mL | 2.3385 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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