| Size | Price | Stock | Qty |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
pCAME does not have a single defined biological target. It possesses multiple functions, including inhibiting melanin synthesis, acting as a fungal metabolite, an anti-inflammatory agent, an antifungal agent, and a plant metabolite. pCAME is a cinnamate ester that may interact with various cellular targets and pathways. Its inhibition of melanin synthesis suggests effects on tyrosinase or melanogenesis-related pathways. Its anti-inflammatory activity suggests modulation of inflammatory signaling pathways. pCAME is an inducer of ectopic tail formation of zebrafish, indicating effects on developmental pathways. The compound's diverse biological activities make it a valuable tool for studying various biological processes.
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| ln Vitro |
pCAME inhibits melanin synthesis in vitro, suggesting effects on tyrosinase or melanogenesis-related pathways. The compound possesses anti-inflammatory and antifungal activities, as demonstrated in cell-based assays. pCAME is a plant metabolite and a fungal metabolite, indicating its role in plant and fungal biology. The compound's anti-inflammatory activity has been characterized by measuring its effects on cytokine production and inflammatory markers. Its antifungal activity has been assessed by standard antifungal susceptibility testing methods. pCAME is an inducer of ectopic tail formation of zebrafish, indicating effects on developmental pathways.
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| ln Vivo |
pCAME has been studied in vivo in zebrafish models, where it induces ectopic tail formation. The compound's effects on development have been characterized in this model system. pCAME's anti-inflammatory and antifungal activities suggest potential therapeutic applications. The compound is a plant metabolite and may have effects on plant physiology. Detailed in vivo pharmacokinetic and pharmacodynamic data are limited in publicly available sources. pCAME is for research use only and is not for human therapeutic use.
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| Enzyme Assay |
pCAME does not have established receptor binding or enzyme inhibition assay protocols. Its activity is assessed by measuring its effects on melanin synthesis, inflammation, and fungal growth. Melanin synthesis inhibition is assessed by measuring melanin production in melanocytes or melanoma cells. Anti-inflammatory activity is assessed by measuring cytokine production and inflammatory markers in immune cells. Antifungal activity is assessed by standard broth microdilution or agar diffusion methods. Physical properties: molecular weight 178.18 g/mol; molecular formula C10H10O3; IUPAC name: methyl (E)-3-(4-hydroxyphenyl)acrylate; appearance: solid; solubility: soluble in DMSO and organic solvents.
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| Cell Assay |
Cellular activity of pCAME is evaluated in various cell types, including melanocytes, melanoma cells, immune cells, and fungal cells. Cells are cultured in appropriate media at 37°C with 5% CO₂ and treated with pCAME at concentrations ranging from 1 to 100 μM for 24-72 hours. Melanin synthesis is assessed by measuring melanin content and tyrosinase activity. Anti-inflammatory activity is assessed by measuring cytokine production (e.g., TNF-α, IL-6) by ELISA. Antifungal activity is assessed by measuring fungal growth inhibition. Cell viability is assessed using MTT or LDH assays. Each experiment includes vehicle controls and appropriate positive controls to validate the assay systems.
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| Animal Protocol |
In vivo efficacy of pCAME is evaluated in zebrafish models and other animal models. The compound is administered in the water or by injection at doses determined by preclinical studies. In zebrafish, ectopic tail formation is assessed by microscopy. In models of inflammation or fungal infection, the compound's effects are assessed by measuring inflammatory markers or fungal burden. Sample sizes typically range from 6-10 animals per group.
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| ADME/Pharmacokinetics |
Molecular Weight: 178.18. Formula: C10H10O3. CAS No.: 19367-38-5. IUPAC Name: methyl (E)-3-(4-hydroxyphenyl)acrylate. Synonyms: pCAME; Methyl trans-p-coumarate; Methyl 4-hydroxycinnamate; Methyl p-coumarate; (E)-4-Hydroxycinnamic acid methyl ester; 4-Coumaric acid methyl ester. Appearance: Solid. Purity: Typically >98%. Solubility: Soluble in DMSO. Storage: Typically at room temperature. pCAME is a naturally occurring cinnamate ester with multiple biological functions.
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| Toxicity/Toxicokinetics |
No comprehensive toxicology data are publicly available for pCAME. The compound is intended for research use only and has not undergone full preclinical toxicology evaluation required for clinical development. As a naturally occurring compound, it is generally considered safe at moderate doses. Standard toxicity studies would include acute toxicity assessment in rodents, repeated dose toxicity studies (14-day and 28-day), and genotoxicity screening (Ames test, micronucleus assay). The compound is for research use only and not for human therapeutic use.
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| References | |
| Additional Infomation |
Methyl 4-coumarate is a cinnamic ester, a methyl ester derivative of 4-coumaric acid. It possesses multiple functions, including inhibiting melanin synthesis, acting as a fungal metabolite, an anti-inflammatory agent, an antifungal agent, and a plant metabolite. It is a cinnamic ester, belonging to the phenolic class of compounds, and is also a methyl ester. Its functions are related to 4-coumaric acid. Methyl 4-hydroxycinnamic acid has been reported in Alpinia blepharocalyx, Gmelina asiatica, and several other organisms with relevant data.
pCAME is also known as Methyl trans-p-coumarate, Methyl 4-hydroxycinnamate, Methyl p-coumarate, (E)-4-Hydroxycinnamic acid methyl ester, and 4-Coumaric acid methyl ester. Its IUPAC name is methyl (E)-3-(4-hydroxyphenyl)acrylate. pCAME is a naturally occurring cinnamate ester found in several plants, including onion and Morinda citrifolia L. pCAME possesses multiple functions, including inhibiting melanin synthesis, acting as a fungal metabolite, an anti-inflammatory agent, an antifungal agent, and a plant metabolite. pCAME is an inducer of ectopic tail formation of zebrafish. No clinical trials have been reported for this compound. pCAME is for research use only. |
| Molecular Formula |
C10H10O3
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| Molecular Weight |
178.18
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| Exact Mass |
178.062
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| CAS # |
19367-38-5
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| Related CAS # |
Methyl p-coumarate;3943-97-3
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| PubChem CID |
5319562
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
306.6±17.0 °C at 760 mmHg
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| Melting Point |
138-140ºC
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| Flash Point |
132.8±13.7 °C
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| Vapour Pressure |
0.0±0.7 mmHg at 25°C
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| Index of Refraction |
1.593
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| LogP |
1.64
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
13
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| Complexity |
190
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| Defined Atom Stereocenter Count |
0
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| SMILES |
COC(=O)/C=C/C1=CC=C(C=C1)O
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| InChi Key |
NITWSHWHQAQBAW-QPJJXVBHSA-N
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| InChi Code |
InChI=1S/C10H10O3/c1-13-10(12)7-4-8-2-5-9(11)6-3-8/h2-7,11H,1H3/b7-4+
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| Chemical Name |
methyl (E)-3-(4-hydroxyphenyl)prop-2-enoate
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| Synonyms |
p CAME; p-CAME; pCAME
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~561.23 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (14.03 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.03 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (14.03 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.6123 mL | 28.0615 mL | 56.1230 mL | |
| 5 mM | 1.1225 mL | 5.6123 mL | 11.2246 mL | |
| 10 mM | 0.5612 mL | 2.8062 mL | 5.6123 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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