| Size | Price | Stock | Qty |
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| 250mg |
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| ln Vitro |
Scaffolds are rescued by pargyline HCl (0.5-2 mM; 24-120 hr; LNCaP-LN3 cells) in a mold- and time-assisted manner [2]. In a quantitatively dependent way, pargyline HCl (0.5-2 mM; 24-48 h; LNCaP) and pargyline HCl (0.5 mM; 24 h; LNCaP-LN3 cells) therapy decreases S phase and enhances G1 phase in cells [2]. LNCaP-LN3 cells treated with 0.5 mM pargyline hydrochloride for 24 hours results in an increase in sterile cells [2]. The administration of 2 mM for 48 hours to LNCaP-LN3 cells results in an upregulation of intracellular caspase-3 and cytochrome c. decrease, although it has no effect on BCL-2 expression alterations [2].
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| ln Vivo |
In unanesthetized spontaneous gradients (SHR), pargyline hydrochloride (10 mg/kg; intravenous) treatment results in a moderate (roughly 20 mmc) but sustained (48 h) contraction reduction, but not in normotensive The middle will not [3]. Arterial pressure is lowered by injecting 200 μg of low-dose pargyline hydrochloride (icv) directly into the brain. The buildup of inhibitory alpha-receptin receptors in the brain appears to be the cause of pargyline hydrochloride's antihypertensive effect in SHR [3].
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| Cell Assay |
Cell Proliferation Assay[2]
Cell Types: LNCaP- LN3 Cell Tested Concentrations: 0.5 mM, 1 mM, 1.5 mM or 2 mM Incubation Duration: 24 hrs (hours), 48 hrs (hours), 72 hrs (hours), 96 hrs (hours) or 120 hrs (hours) Experimental Results: Time and dose dependent Sexual intercourse inhibits the proliferation of prostate cancer cells. Cell cycle analysis [2] Cell Types: LNCaP-LN3 Cell Tested Concentrations: 0.5 mM, 2 mM Incubation Duration: 24 hrs (hours), 48 hrs (hours) Experimental Results: The proportion of cells in S phase diminished and the proportion of G1 phase increased. Apoptosis analysis[2] Cell Types: LNCaP-LN3 Cell Tested Concentrations: 0.5 mM Incubation Duration: 24 hrs (hours) Experimental Results: Increased apoptotic cells. Western Blot Analysis[2] Cell Types: LNCaP-LN3 Cell Tested Concentrations: 2 mM Incubation Duration: 48 hrs (hours) Experimental Results: Induction of increased cytochrome c and diminished caspase-3. |
| References |
[1]. C J Fowler, et al. The nature of the inhibition of rat liver monoamine oxidase types A and B by the acetylenic inhibitors clorgyline, l-deprenyl and pargyline. Biochem Pharmacol. 1982 Nov 15;31(22):3555-61.
[2]. Hyung Tae Lee, et al. Effects of the monoamine oxidase inhibitors pargyline and tranylcypromine on cellular proliferation in human prostate cancer cells. Oncol Rep. 2013 Oct;30(4):1587-92. [3]. Fuentes JA, et al. Central mediation of the antihypertensive effect of pargyline in spontaneously hypertensive rats. Eur J Pharmacol. 1979 Jul 15;57(1):21-7. |
| Molecular Formula |
C11H14CLN
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| Molecular Weight |
195.69
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| CAS # |
306-07-0
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| Related CAS # |
Pargyline;555-57-7
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| SMILES |
CN(CC#C)CC1=CC=CC=C1.Cl
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| InChi Key |
BCXCABRDBBWWGY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H13N.ClH/c1-3-9-12(2)10-11-7-5-4-6-8-11/h1,4-8H,9-10H2,2H31H
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| Chemical Name |
N-benzyl-N-methylprop-2-yn-1-aminehydrochloride
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| Synonyms |
Pargyline Hydrochloride N-benzyl-N-methylprop-2-yn-1-amine hydrochloride Eutonyl-ten N-Methyl-N-propargylbenzylamine hydrochloride Pargyline HCl Pargyline
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~638.77 mM)
H2O : ~100 mg/mL (~511.01 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (10.63 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (10.63 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (10.63 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 25 mg/mL (127.75 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with heating and sonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.1101 mL | 25.5506 mL | 51.1012 mL | |
| 5 mM | 1.0220 mL | 5.1101 mL | 10.2202 mL | |
| 10 mM | 0.5110 mL | 2.5551 mL | 5.1101 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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