| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Palmitodiolein does not target a specific receptor or enzyme but functions as a triglyceride and energy storage molecule. As a triacylglycerol found in vegetable oils, it is involved in lipid metabolism and energy homeostasis. The compound serves as a substrate for lipases and is hydrolyzed to release free fatty acids and glycerol. Its presence in vegetable oils makes it relevant for studies on dietary fats, lipid digestion, absorption, and metabolism.
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| ln Vitro |
In vitro, Palmitodiolein is used as a lipid standard and substrate for studying lipid metabolism. As a triacylglycerol present in vegetable oils, it can be used in lipase activity assays to measure the hydrolysis of triglycerides. The compound's fatty acid composition (one palmitic acid and two oleic acids) makes it useful for studying the specificity of lipases and the metabolism of different fatty acids. It is also used as a reference standard in lipid analysis by chromatography.
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| ln Vivo |
In vivo, Palmitodiolein is a naturally occurring triglyceride found in vegetable oils. As a dietary fat, it is digested and absorbed in the gastrointestinal tract, transported in lipoproteins, and metabolized in various tissues. Its presence in the diet contributes to energy intake and lipid metabolism. The compound can be used in nutritional studies to investigate the effects of different fatty acid compositions on health and disease. Further in vivo studies are needed to fully characterize its metabolic effects.
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| Enzyme Assay |
For in vitro enzyme assays, Palmitodiolein is used as a substrate in lipase activity assays. The compound is incubated with lipase enzymes at various concentrations in appropriate buffer systems. Lipase activity is quantified by measuring the release of free fatty acids using colorimetric, titrimetric, or fluorometric methods. The specificity of different lipases for Palmitodiolein can be assessed by comparing hydrolysis rates. Standard assay conditions include physiological pH and temperature with appropriate emulsifiers to facilitate substrate accessibility.
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| Cell Assay |
For in vitro cellular experiments, Palmitodiolein can be added to cell culture media to study its effects on lipid metabolism and cellular function. Cells such as adipocytes, hepatocytes, or intestinal cells are cultured in appropriate media and treated with various concentrations of the triglyceride. Cellular uptake, lipid accumulation, and metabolism are assessed using biochemical assays. The compound's effects on cell viability, lipid droplet formation, and gene expression related to lipid metabolism are evaluated.
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| Animal Protocol |
For in vivo animal experiments, Palmitodiolein can be administered to rodents via oral gavage as part of dietary interventions. The compound's effects on lipid metabolism, energy homeostasis, and metabolic health can be evaluated in models of obesity, diabetes, or dyslipidemia. Typical doses may range from 1 to 100 mg/kg depending on the study objectives. Parameters such as body weight, food intake, lipid profiles, and glucose metabolism are measured. Tissue samples are collected for analysis of lipid content and metabolic markers.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Palmitodiolein are not extensively characterized, as it is a dietary triglyceride rather than a therapeutic agent. As a triglyceride, it is digested in the gastrointestinal tract by lipases to release free fatty acids and glycerol, which are then absorbed and metabolized. The compound's pharmacokinetics would depend on its digestion, absorption, and incorporation into lipoproteins. Its half-life in circulation would depend on its clearance by tissues. Further studies would be needed to fully characterize its metabolic fate.
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| Toxicity/Toxicokinetics |
Toxicological data for Palmitodiolein are limited, as it is a naturally occurring triglyceride found in vegetable oils. It is generally recognized as safe for consumption as part of the diet. At high doses, triglycerides can contribute to obesity and metabolic disorders. Standard toxicological assessments would include evaluation of its effects on lipid metabolism and organ function. As with all research chemicals, appropriate safety precautions should be taken when handling Palmitodiolein.
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| Additional Infomation |
1,2-Dioleoyl-3-palmitoylglycerol is a triglyceride in which the acyl groups at positions 1 and 2 are oleoyl groups, and the acyl group at position 3 is a palmitoyl group. It is functionally related to hexadecanoic acid and oleic acid. 1,2-Dioleoyl-3-palmitoylglycerol has been reported in Celastrus paniculatus, and relevant data are available. See also: Coix lacryma-jobi seeds (partial).
Palmitodiolein is a research compound used as a lipid standard and for studying lipid metabolism. No clinical trials or regulatory approvals have been reported for this compound as a therapeutic agent. It is available from various chemical suppliers for research purposes only. The compound is a triacylglycerol present in vegetable oils, composed of one palmitic acid and two oleic acid molecules. |
| Molecular Formula |
C55H102O6
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|---|---|
| Molecular Weight |
859.395
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| Exact Mass |
858.767
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| CAS # |
2190-30-9
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| PubChem CID |
25240174
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| Appearance |
Typically exists as solid at room temperature
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| Density |
0.9±0.1 g/cm3
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| Boiling Point |
802.2±45.0 °C at 760 mmHg
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| Flash Point |
298.4±28.8 °C
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| Vapour Pressure |
0.0±2.8 mmHg at 25°C
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| Index of Refraction |
1.473
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| LogP |
23.17
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
52
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| Heavy Atom Count |
61
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| Complexity |
989
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCCCCCCCCCCCCC(OCC(OC(CCCCCCC/C=C\CCCCCCCC)=O)COC(CCCCCCC/C=C\CCCCCCCC)=O)=O
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| InChi Key |
JFISYPWOVQNHLS-LBXGSASVSA-N
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| InChi Code |
InChI=1S/C55H102O6/c1-4-7-10-13-16-19-22-25-27-30-33-36-39-42-45-48-54(57)60-51-52(50-59-53(56)47-44-41-38-35-32-29-24-21-18-15-12-9-6-3)61-55(58)49-46-43-40-37-34-31-28-26-23-20-17-14-11-8-5-2/h25-28,52H,4-24,29-51H2,1-3H3/b27-25-,28-26-
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| Chemical Name |
[3-hexadecanoyloxy-2-[(Z)-octadec-9-enoyl]oxypropyl] (Z)-octadec-9-enoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~116.36 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (1.45 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 1.25 mg/mL (1.45 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.25 mg/mL (1.45 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.1636 mL | 5.8180 mL | 11.6360 mL | |
| 5 mM | 0.2327 mL | 1.1636 mL | 2.3272 mL | |
| 10 mM | 0.1164 mL | 0.5818 mL | 1.1636 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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