| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| Targets |
Oxazine 1 perchlorate does not have a specific biological target. It functions as a fluorescent dye. It is used to label and visualize specific molecules or structures within cells and tissues by binding to target molecules.
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|---|---|
| ln Vitro |
In diluted aqueous solutions, oxazine 1 perchlorate (OX1) absorbs and emits light at roughly 653 and 666 nm, respectively. Numerous polar solvents can dissolve it. Oxazine 1 perchlorate typically exhibits a high absorption band (λmax) next to a shoulder of shorter wavelengths in its spectra. At concentrations less than about 1 × 10-4 M, oxazine 1 perchlorate appears to exist in aqueous solutions nearly in its monomeric state. In polar anisotropic media, Oxazine 1 perchlorate exhibits only one fluorescence band, as would be predicted and comparable to polar common liquid solvents [1].
In cell-free systems, Oxazine 1 perchlorate is used as a fluorescent probe. Its spectral properties, such as absorption and emission maxima, are characterized in various solvents. Its stability and quantum yield are measured. In vitro, Oxazine 1 perchlorate is used to stain cells and tissues. It binds to target molecules or structures and emits fluorescence when excited by a specific wavelength of light. It is used in fluorescence microscopy and flow cytometry. |
| ln Vivo |
In vivo applications are limited. As a dye, it is primarily used for in vitro labeling. It may be used in ex vivo imaging or in some in vivo imaging applications, but its utility is mainly as a research tool.
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| Enzyme Assay |
The dye's photophysical properties are characterized in solution. Its absorbance and fluorescence spectra are recorded. Its molar extinction coefficient and fluorescence quantum yield are determined. Its binding to target molecules can be studied by fluorescence spectroscopy.
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| Cell Assay |
Cells are incubated with Oxazine 1 perchlorate. The dye enters the cells and binds to its targets. The stained cells are then visualized using a fluorescence microscope or analyzed by flow cytometry. The excitation and emission wavelengths are used for detection.
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| Animal Protocol |
In vivo imaging studies may involve administering the dye to animals and visualizing its distribution using imaging systems. However, its use in vivo is not as common as its in vitro applications. Its pharmacokinetics are not well-characterized.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not relevant for Oxazine 1 perchlorate as it is not a therapeutic agent. It is a research tool used for staining and imaging. Its stability and fluorescence properties are the primary considerations for its use.
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| Toxicity/Toxicokinetics |
Oxazine 1 perchlorate is generally handled with standard laboratory safety precautions for chemicals and dyes. It is not intended for therapeutic use, and its toxicity profile is not a primary concern for its applications as a research reagent.
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| References | |
| Additional Infomation |
Oxazine-1 perchlorate is an organic perchlorate that can be used as a fluorescent dye. It contains an oxazine-1 group.
Oxazine 1 perchlorate is a fluorescent dye used in various research applications, including cell staining and fluorescence microscopy. It is a valuable tool for visualizing cellular structures. It is not a pharmaceutical drug. |
| Molecular Formula |
C20H26N3O+
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|---|---|
| Molecular Weight |
324.43994
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| Exact Mass |
423.156
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| CAS # |
24796-94-9
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| Related CAS # |
47367-75-9 (Parent)
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| PubChem CID |
90611
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| Appearance |
Light green to green solid powder
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| LogP |
3.805
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
29
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| Complexity |
629
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
PKZWDLHLOBYXKV-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C20H26N3O.ClHO4/c1-5-22(6-2)15-9-11-17-19(13-15)24-20-14-16(23(7-3)8-4)10-12-18(20)21-17;2-1(3,4)5/h9-14H,5-8H2,1-4H3;(H,2,3,4,5)/q+1;/p-1
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| Chemical Name |
[7-(diethylamino)phenoxazin-3-ylidene]-diethylazanium;perchlorate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~117.96 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.90 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.90 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0822 mL | 15.4112 mL | 30.8223 mL | |
| 5 mM | 0.6164 mL | 3.0822 mL | 6.1645 mL | |
| 10 mM | 0.3082 mL | 1.5411 mL | 3.0822 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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