| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
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| ln Vitro |
Oleanolic acid (OA) increases the quantity of miR-122 while inhibiting lung cancer cell proliferation in a dose- and time-dependent manner. For OA processing, two matching miR-122 targets are needed: CCNG1 and MEF2D [1]. In normal tissue-derived cells, OA activates autophagy without causing harm. KRAS conversion into normal cell proliferation is inhibited by OA-induced autophagy, which also hinders the cells' logical and qualitatively independent growth [2].
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|---|---|
| ln Vivo |
Additionally, studies using mouse models demonstrate that OA induces autophagy, which prevents the formation of xenografts produced from KRAS-transformed breast epithelial MCF10A [2]. In every colon that was tested, OA caused the activation of MAPK fluorescence, including p-38 MAPK, JNK, and ERK, in a way that was dose- and time-dependent. By increasing their phosphorylation, OA-induced p38 MAPK activation limits Bcl-2 activity and encourages the translocation of Bax and Bim into the mitochondria. Reactive oxygen species (ROS)-dependent ASK1 activation is induced by OA, and this cardiovascular event occurs in the pancreatic p38 MAPK cells [3]. This further demonstrates that A549 tumors with p38 MAPK inactivation are immune to the growth-inhibitory impact of OA [3]. Pro-inflammatory and pro-fibrotic cytokines were markedly decreased in EAM animals treated with OA, although Treg cell counts and IL-10 and IL-35 production were markedly elevated [4].
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| References |
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| Additional Infomation |
Oleanolic acid is a pentacyclic triterpenoid that is olean-12-en-28-oic acid substituted by a beta-hydroxy group at position 3. It has a role as a plant metabolite. It is a pentacyclic triterpenoid and a hydroxy monocarboxylic acid. It is a conjugate acid of an oleanolate. It derives from a hydride of an oleanane.
Oleanolic acid has been reported in Salvia miltiorrhiza, Sambucus chinensis, and other organisms with data available. A pentacyclic triterpene that occurs widely in many PLANTS as the free acid or the aglycone for many SAPONINS. It is biosynthesized from lupane. It can rearrange to the isomer, ursolic acid, or be oxidized to taraxasterol and amyrin. See also: Holy basil leaf (part of); Paeonia lactiflora root (part of); Jujube fruit (part of) ... View More ... |
| Molecular Formula |
C30H48O3
|
|---|---|
| Molecular Weight |
456.7003
|
| Exact Mass |
456.36
|
| CAS # |
508-02-1
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| PubChem CID |
10494
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| Appearance |
White to off-white solid powder
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
553.5±50.0 °C at 760 mmHg
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| Melting Point |
>300 °C(lit.)
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| Flash Point |
302.6±26.6 °C
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| Vapour Pressure |
0.0±3.4 mmHg at 25°C
|
| Index of Refraction |
1.557
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| LogP |
9.06
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| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
|
| Heavy Atom Count |
33
|
| Complexity |
885
|
| Defined Atom Stereocenter Count |
8
|
| SMILES |
C[C@]12CC[C@@H](C([C@@H]1CC[C@@]3([C@@H]2CC=C4[C@]3(CC[C@@]5([C@H]4CC(CC5)(C)C)C(=O)O)C)C)(C)C)O
|
| InChi Key |
MIJYXULNPSFWEK-GTOFXWBISA-N
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| InChi Code |
InChI=1S/C30H48O3/c1-25(2)14-16-30(24(32)33)17-15-28(6)19(20(30)18-25)8-9-22-27(5)12-11-23(31)26(3,4)21(27)10-13-29(22,28)7/h8,20-23,31H,9-18H2,1-7H3,(H,32,33)/t20-,21-,22+,23-,27-,28+,29+,30-/m0/s1
|
| Chemical Name |
(4aS,6aR,6aS,6bR,8aR,10S,12aR,14bS)-10-hydroxy-2,2,6a,6b,9,9,12a-heptamethyl-1,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydropicene-4a-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMF : 45.45 mg/mL (~99.52 mM)
DMSO : ~5 mg/mL (~10.95 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 0.5 mg/mL (1.09 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 0.5 mg/mL (1.09 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 0.5 mg/mL (1.09 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1896 mL | 10.9481 mL | 21.8962 mL | |
| 5 mM | 0.4379 mL | 2.1896 mL | 4.3792 mL | |
| 10 mM | 0.2190 mL | 1.0948 mL | 2.1896 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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