| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
NP213 (500-1000 μg/mL; 18 hours) increases the quantity of T. rubrum NCPF0118 cells that are stained with PI in the samples. According to these findings, NP213 has fungicidal properties and acts through membrane permeabilization[1]. NP213 exhibits varying minimum inhibitory concentrations (MICs) in relation to T. rubrum NCPF0118, with the MICs being contingent on the keratin source. For NP213 in 1640 medium containing human nail keratin, human skin keratin, and lamb's wool keratin, respectively, the MIC values are 16–32 mg/L, 125 mg/L, and 250 mg/L[1].Great action is shown by NP213 TFA (2–3 hours; 0–8 μg/ml) against yeast that is clinically relevant, such as Candida species, Cryptococcus species, and Trichosporon species. The median MIC100 values for all 122 yeast isolates were found to be 1-2 µg/ml [3].
|
|---|---|
| ln Vivo |
In mice, NP213 TFA (25 mg/kg) is well tolerated. NP213 has a half-life of roughly 4.5 hours and is well-tolerated and effective in murine models of acute disseminated candidiasis[3].
|
| Cell Assay |
Cell Cytotoxicity Assay[3]
Cell Types: Clinically relevant yeast isolates Tested Concentrations: 0 μg/ml; 0.125 μg/ml; 0.25 μg/ml; 0.5 μg/ml; 1 μg/ml; 2 μg/ml; 4 μg/ml; 8 μg/ml Incubation Duration: 2 h, 2.5 h, 4 h Experimental Results: diminished isolates growth as a dose-dependent manner. |
| References |
|
| Molecular Formula |
C??H??F?N??O?
|
|---|---|
| Molecular Weight |
1207.32
|
| Related CAS # |
NP213;942577-31-3
|
| Appearance |
White to off-white solid powder
|
| Synonyms |
NP213 TFA; NP213 TFA
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.07 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.07 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (2.07 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 100 mg/mL (82.83 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.8283 mL | 4.1414 mL | 8.2828 mL | |
| 5 mM | 0.1657 mL | 0.8283 mL | 1.6566 mL | |
| 10 mM | 0.0828 mL | 0.4141 mL | 0.8283 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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