| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Nonylacridine Orange targets cardiolipin, a phospholipid located in the inner mitochondrial membrane. As a fluorescent probe, it does not have a pharmacological receptor target but rather binds specifically to cardiolipin through electrostatic and hydrophobic interactions. Upon binding to cardiolipin, the dye's excitation and emission wavelengths shift from 496/525 nm to 450/640 nm, respectively.
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| ln Vitro |
In vitro, Nonylacridine Orange is used as a fluorescent probe to label cardiolipin in isolated mitochondria and cultured cells. It exhibits high specificity for cardiolipin and is commonly used to assess mitochondrial mass, function, and membrane potential. The compound's fluorescence can be detected using flow cytometry or fluorescence microscopy, providing a quantitative measure of cardiolipin content.
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| ln Vivo |
To measure cardiolipin in isolated mitochondria, Acridine orange 10-nonyl bromide, a fluorescent marker for cardiolipin (λex: 489 nm, λem: 525 nm), can be utilized [1]. Acridine orange 10-nonyl bromide and cardiolipin interact to shift the wavelengths of the dye's excitation and emission, from 496 and 525 nm to 450 and 640 nm, respectively. The red hue measured at 640 nm changes depending on the composition of the grating body when phospholipids in thin-walled vesicles (0 to 30 μM) and other acidic species added to acridine orange 10-nonyl bromide (45 μM) are added. emission of fluorescence [2].
Nonylacridine Orange is not a therapeutic drug and does not have pharmacological in vivo activity. It is a research tool used exclusively for in vitro and ex vivo applications to study mitochondrial biology. Its utility lies in its ability to specifically label mitochondria in fixed or live cells, enabling the study of mitochondrial dynamics and function. |
| Enzyme Assay |
Nonylacridine Orange is a fluorescent probe and does not have enzyme/receptor binding activity in the context of pharmacological assays. Its interaction with cardiolipin can be studied in cell-free systems using isolated mitochondria or liposomes containing cardiolipin. Fluorescence spectroscopy is used to monitor the shift in excitation and emission wavelengths upon binding to cardiolipin.
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| Cell Assay |
In vitro cellular assays for Nonylacridine Orange are performed using cultured cells or isolated mitochondria. Cells are incubated with the probe, and fluorescence is measured using flow cytometry or fluorescence microscopy. The probe's emission at 525 nm is typically monitored, and the shift to 640 nm upon cardiolipin binding can also be detected. These assays are used to assess mitochondrial mass and function.
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| Animal Protocol |
In vivo animal studies are not typically performed with Nonylacridine Orange, as it is a fluorescent probe for in vitro applications rather than a therapeutic compound. It is not designed for administration to animals for pharmacological evaluation. Its use is strictly limited to laboratory settings for studying mitochondrial biology in isolated cells and tissues.
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| ADME/Pharmacokinetics |
Nonylacridine Orange is not a therapeutic drug and does not have pharmacokinetic properties relevant to drug development. Its chemical properties include a molecular formula of C₂₆H₃₈BrN₃ and a molecular weight of 472.50 g/mol. The compound is soluble in DMSO and should be stored at 4°C protected from light. Stock solutions should be aliquoted and stored at -20°C.
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| Toxicity/Toxicokinetics |
Toxicology studies are not conducted for Nonylacridine Orange as a therapeutic agent, as it is a research reagent rather than a drug. Standard laboratory safety precautions should be followed when handling this compound, including the use of personal protective equipment. The compound is for research use only and not for human use.
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| References | |
| Additional Infomation |
Nonylacridine Orange (CAS: 75168-11-5), also known as Acridine Orange 10-nonyl bromide, is a fluorescent probe specific for cardiolipin in mitochondria. It has an absorption wavelength of 489 nm and an emission wavelength of 525 nm. The compound is commonly used in flow cytometry and fluorescence microscopy to study mitochondrial properties. It is for research use only and not for human therapeutic use.
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| Molecular Formula |
C26H38BRN3
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|---|---|
| Molecular Weight |
471.52
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| Exact Mass |
471.225
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| CAS # |
75168-11-5
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| PubChem CID |
2762576
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| Appearance |
Brown to red solid powder
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| Melting Point |
≥250ºC(lit.)
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| LogP |
3.167
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
30
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| Complexity |
431
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
DRBHTUDHPPBMCD-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C26H38N3.BrH/c1-6-7-8-9-10-11-12-17-29-25-19-23(27(2)3)15-13-21(25)18-22-14-16-24(28(4)5)20-26(22)29;/h13-16,18-20H,6-12,17H2,1-5H3;1H/q+1;/p-1
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| Chemical Name |
3-N,3-N,6-N,6-N-tetramethyl-10-nonylacridin-10-ium-3,6-diamine;bromide
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| Synonyms |
Acridine Orange 10-nonyl bromide; 10-Nonyl acridine Orange
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~33.33 mg/mL (~70.54 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.67 mg/mL (3.53 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.67 mg/mL (3.53 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1208 mL | 10.6040 mL | 21.2080 mL | |
| 5 mM | 0.4242 mL | 2.1208 mL | 4.2416 mL | |
| 10 mM | 0.2121 mL | 1.0604 mL | 2.1208 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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