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    NMDA (N-Methyl-D-aspartic acid)
    NMDA (N-Methyl-D-aspartic acid)

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V1086
    CAS #: 6384-92-5Purity ≥98%

    Description: NMDA (N-Methyl-D-aspartic acid; LC488A; LC488 A; LC488-A; LC 488A; LC-488A) is an aspartic acid analog with an N-methyl substituent and D-configuration and a glutamate-like excitatory chemical substance. It acts as a potent and specific agonist for NMDA receptor mimicking the action of glutamate, which is a neurotransmitter acting at NMDA receptors.  

    References: Curr Opin Neurobiol. 2001 Jun;11(3):327-35; Nature. 1993 Aug 5;364(6437):535-7.

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    Molecular Weight (MW)147.13
    FormulaC5H9NO4
    CAS No.6384-92-5
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 5 mg/mL (33.98 mM) 
    Water: 30 mg/mL (203.9 mM)
    Ethanol: <1 mg/mL
    Solubility (In vivo)

    Chemical Name: N-Methyl-D-aspartic acid

    InChi Key: RVHOBHMAPRVOLO-SCSAIBSYSA-N

    InChi Code: InChI=1S/C6H10O4/c1-2-4(6(9)10)3-5(7)8/h4H,2-3H2,1H3,(H,7,8)(H,9,10)/t4-/m1/s1

    SMILES: O=C(O)C[[email protected]](C(O)=O)CC

    SynonymsLC488A; LC488 A; LC488-A;  LC 488A; LC-488A


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    In Vitro

    In vitro activity: NMDA is an excitatory amino acid neurotransmitter, which only binds to the NMDA receptor without effecting other glutamate receptors (such as those for AMPA and kainate). NMDA specifically binds to the NR2 subunits of NMDA receptor, and then stimulates the open of non-specific cation channel which can allow the passage of Ca2+ and Na+ into the cell and K+ out of the cell. Activation of the NMDA receptor is able to produce the excitatory postsynaptic potential (EPSP), and trigger the increase of intracellular Ca2+ content which may further take participating in various signaling pathways. NMDA receptor plays a key role in a wide range of physiological (e.g. long-term potentiation and neuronal plasticity) and pathological processes (e.g. excitotoxicity and epilepsy).


    Kinase Assay: Adrenal membranous homogenate suspensions are incubated with 10 nM [3H]Glu in 500/zl 50 mM Tris-acetate buffer (pH 7.4) at 2°C or 30°C in the presence and absence of various compounds. Incubation is terminated by the addition of 3 mL ice-cold buffer and subsequent filtration through a Whatman GF/B glass filter under a constant vacuum of 15 mm Hg. After washing the filter 4 times with 3 mL icecold buffer, the radioactivity trapped on the filter is measured by a liquid scintillation spectrometer using 5 mL modified Triton-toluene scintillant at a counting efficiency of 40-42%. The radioactivity found in the presence of 1 mM non-radioactive Glu is subtracted from each experimental value to obtain the specific binding of [3H]GIu in accordance with the y-aminobutyric acid (GABA) receptor binding assay system. The kinetic parameters of [3H]GIu binding, Kd and Bma x, are calculated by Scatchard analysis of the specific binding using a personal computer with a programme for non-linear regression analysis developed in our own laboratory.

    In VivoNMDA (0.2 nM) shows significant effects on MF, IF, IL, and EL, respectively, decreasing the mount and intromission frequencies, and shortening the intromission and ejaculation latencies. NMDA and AP-5 significantly, respectively, facilitates and inhibits the ejaculatory behavior during the copulation testing 30 min. Bilateral microinjection of NMDA into PVN significantly increases the baseline LSNA, the peaking increment of LSNA occurred within 5 min from the time of NMDA microinjected into PVN
    Animal modelRats: Thirty male rats are paired with different receptive females for a total of three times (once every 3 days) a week prior to the experiment, only the males that ejaculated at least three times during this period are included. After selecting the male rats with normal ejaculatory ability. Saline (100 nL), NMDA (0.20 nmol in 100 nL saline), and AP-5 (10.0 nmol in 100 nL saline) are adminitration into the bilateral PVN of each male rat in random order. After 5 min, the behavioral testing is performed and recorded as described above. Copulatory behaviors occur once a week and the entire experiment lasted 4 weeks.
    Formulation & Dosage0.20 nmol in 100 nL saline
    References

    Curr Opin Neurobiol. 2001 Jun;11(3):327-35.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

    NMDA (N-Methyl-D-aspartic acid)




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