| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Biotin-binding proteins (avidin, streptavidin, neutravidin) via the biotin moiety; the nitroso group provides reactivity toward thiols, amines, and other nucleophilic residues for covalent protein modification.
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|---|---|
| ln Vitro |
In vitro activity is based on its ability to form covalent adducts with nucleophilic residues on proteins. The biotin moiety retains high-affinity binding to streptavidin (Kd ~10-¹⁵ M), allowing detection and capture of nitroso-modified proteins. The nitroso group enables selective labeling of cysteine and lysine residues under physiological conditions.
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| ln Vivo |
No direct in vivo activity; functions as a biochemical probe for labeling and detecting proteins in complex biological mixtures. It is used in proteomics, redox biology, and protein modification studies to investigate nitrosylation and oxidative stress pathways.
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| Enzyme Assay |
Streptavidin binding assay: Nitrosobiotin (0.01-100 nM) is incubated with HRP-conjugated streptavidin (1:5000) in PBS-T buffer for 1 hour at room temperature. The complex is captured on a biotin-coated plate or detected by ELISA. For nitroso reactivity: Nitrosobiotin (10-100 uM) is incubated with model proteins (e.g., BSA, 10 uM) in PBS pH 7.4 for 2 hours at 37degC; adduct formation is analyzed by SDS-PAGE and Western blot with anti-biotin antibody.
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| Cell Assay |
Protein labeling assay: Cells are lysed in RIPA buffer and incubated with nitrosobiotin (10-100 uM) for 1-2 hours at 37degC. Labeled proteins are captured by streptavidin-agarose beads, eluted by boiling in SDS sample buffer, and analyzed by SDS-PAGE followed by Western blot with antibodies of interest. Alternatively, proteins are separated by 2D electrophoresis and detected with streptavidin-HRP.
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| Animal Protocol |
No in vivo animal models are applicable as nitrosobiotin is a research probe. It may be used in ex vivo tissue labeling: tissues are homogenized and incubated with nitrosobiotin, and labeled proteins are identified by mass spectrometry. Biodistribution studies in mice could be performed using radiolabeled nitrosobiotin, though not reported.
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| ADME/Pharmacokinetics |
Not applicable as a therapeutic; nitrosobiotin is used as a research reagent. No pharmacokinetic data are available. As a small molecule (MW 273.31), it is expected to have moderate membrane permeability and be metabolized or cleared rapidly.
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| Toxicity/Toxicokinetics |
No toxicity data are available for nitrosobiotin. As a biotin derivative, it is expected to have low toxicity. Standard laboratory safety precautions should be followed when handling this compound. No genotoxicity or carcinogenicity studies have been reported.
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| References | |
| Additional Infomation |
Nitrosobiotin is a research compound, not a drug. It is used as a chemical probe for studying protein nitrosylation, redox biology, and covalent modification of proteins. The nitroso group enables the labeling and identification of S-nitrosylated proteins, expanding the utility of the biotin-streptavidin system into dynamic and covalent biochemical applications.
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| Molecular Formula |
C10H15N3O4S
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|---|---|
| Molecular Weight |
273.3088
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| Exact Mass |
273.078
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| CAS # |
56859-26-8
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| PubChem CID |
191424
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| Appearance |
Light yellow to yellow solid powder
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| LogP |
0.768
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
18
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| Complexity |
365
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1C2C(C(S1)CCCCC(=O)O)NC(=O)N2N=O
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| InChi Key |
IFUXQNNMTPBRPW-ZKWXMUAHSA-N
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| InChi Code |
InChI=1S/C10H15N3O4S/c14-8(15)4-2-1-3-7-9-6(5-18-7)13(12-17)10(16)11-9/h6-7,9H,1-5H2,(H,11,16)(H,14,15)/t6-,7-,9-/m0/s1
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| Chemical Name |
5-[(3aR,6S,6aS)-3-nitroso-2-oxo-3a,4,6,6a-tetrahydro-1H-thieno[3,4-d]imidazol-6-yl]pentanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~180 mg/mL (~658.59 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 4.5 mg/mL (16.46 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 45.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 4.5 mg/mL (16.46 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 45.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6588 mL | 18.2942 mL | 36.5885 mL | |
| 5 mM | 0.7318 mL | 3.6588 mL | 7.3177 mL | |
| 10 mM | 0.3659 mL | 1.8294 mL | 3.6588 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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