| Size | Price | |
|---|---|---|
| 500mg | ||
| Other Sizes |
| Targets |
Ninhydrin does not have a specific biological target, as it is primarily used as a chemical reagent for the detection of primary amines. It reacts with the α-amino group of amino acids and peptides to form a colored imino derivative, which can be quantified spectrophotometrically. The reaction involves the oxidative deamination of the amino acid, leading to the formation of an aldehyde and the reduced form of ninhydrin, which then reacts with another molecule of ninhydrin and ammonia to form the purple pigment. This reaction is highly specific for primary amines, making ninhydrin a valuable tool in analytical chemistry.
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|---|---|
| ln Vitro |
Protein amino acid analysis uses ninhydrin. Proline is the only amino acid that cannot be hydrolyzed and react with ninhydrin. The amino acids are separated by chromatography and then measured using colorimetry. Indione dione diamine is the blue or purple reaction product that results from the interaction of ninhydrin with primary and secondary amines.
In vitro, Ninhydrin is widely used as a chromogenic analytical probe for the quantification of amino acids and proteins. The ninhydrin reaction is the basis for the standard amino acid analysis method, where amino acids are separated by chromatography and then detected by post-column derivatization with ninhydrin. The intensity of the purple color is proportional to the concentration of the amino acid, and the absorbance is measured at 570 nm. Ninhydrin is also used in protein quantification assays and in the detection of peptides in biological samples. |
| ln Vivo |
In vivo, Ninhydrin is not used as a therapeutic agent; it is a chemical reagent used in analytical and forensic applications. However, the detection of amino acids and peptides in biological samples using ninhydrin has important implications for clinical diagnostics and biomedical research. For example, the measurement of amino acid levels in plasma and urine can be used to diagnose metabolic disorders. Ninhydrin is also used in forensic science to develop latent fingerprints on porous surfaces.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays are not applicable to Ninhydrin, as it is a chemical reagent rather than a pharmacologically active compound. However, its use in analytical chemistry involves the reaction with primary amines. The assay is performed by mixing the sample containing primary amines with ninhydrin in a buffer solution and heating the mixture to 100°C for a few minutes. The resulting purple color is measured spectrophotometrically at 570 nm. The intensity of the color is proportional to the concentration of the primary amine.
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| Cell Assay |
In vitro cellular experiments are not applicable to Ninhydrin, as it is a chemical reagent rather than a biologically active compound. However, it can be used to detect amino acids in cell culture media or cell lysates. The sample is deproteinized, and the supernatant is reacted with ninhydrin. The absorbance is measured at 570 nm, and the concentration of amino acids is calculated using a standard curve. This method is simple and widely used for the determination of total amino acid content in biological samples.
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| Animal Protocol |
In vivo animal studies are not applicable to Ninhydrin, as it is a chemical reagent rather than a therapeutic agent. However, it can be used to measure amino acid levels in plasma, urine, or tissue extracts from animals. The samples are deproteinized, and the supernatant is reacted with ninhydrin. The absorbance is measured at 570 nm. This method is used in metabolic studies to assess amino acid metabolism and nutritional status.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to Ninhydrin, as it is a chemical reagent rather than a therapeutic agent. The compound is not designed to be absorbed or distributed in the body. It is used as an analytical reagent in vitro. If handled improperly, it can be absorbed through the skin or inhaled, but its pharmacokinetics in the body have not been characterized.
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| Toxicity/Toxicokinetics |
The toxicity of Ninhydrin has been evaluated for safe handling in the laboratory. The compound is considered to have low acute toxicity, but it can be irritating to the skin, eyes, and respiratory tract. Prolonged exposure to light should be avoided, as it can cause decomposition. The compound yields highly fluorescent ternary compounds with aldehydes and primary amines. Standard safety precautions, including the use of gloves and eye protection, should be followed when handling this compound.
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| References |
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| Additional Infomation |
Ninhydrin is a white to pale yellow crystal or powder. It becomes anhydrous and turns red at 257-266 °F (121-130 °C). (NTP, 1992) Ninhydrin belongs to the indanone class of compounds and is a compound of indan-1,3-dione with two hydroxyl substituents at the 2-position. It is a colorimetric indicator and a human metabolite. It belongs to the indanone class, β-diketones, aromatic ketones, and ketone hydrates. 2,2-Dihydroxy-1H-indan-1,3-(2H)-dione. It is toxic to the skin and mucous membranes. It is used in the chemical analysis of peptide bonds, such as protein determination, and has radiosensitizing effects.
Ninhydrin is a well-known chemical reagent widely used for the detection of amino acids, peptides, and amines. It reacts with primary amines to produce a deep blue or purple color, known as Ruhemann's purple. Ninhydrin is a critical tool in protein analysis, chromatography, and forensic science, especially for developing latent fingerprints on porous surfaces such as paper. It is also used in spectrophotometric grade for various analytical applications. The compound's specificity for primary amines and its ease of use make it an indispensable reagent in analytical chemistry. |
| Molecular Formula |
C9H6O4
|
|---|---|
| Molecular Weight |
178.14
|
| Exact Mass |
178.026
|
| CAS # |
485-47-2
|
| PubChem CID |
10236
|
| Appearance |
Off-white to light yellow solid powder
|
| Density |
1.7±0.1 g/cm3
|
| Boiling Point |
449.3±45.0 °C at 760 mmHg
|
| Melting Point |
250 °C (dec.)(lit.)
|
| Flash Point |
239.7±25.2 °C
|
| Vapour Pressure |
0.0±1.2 mmHg at 25°C
|
| Index of Refraction |
1.737
|
| LogP |
1.07
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
13
|
| Complexity |
243
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
FEMOMIGRRWSMCU-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C9H6O4/c10-7-5-3-1-2-4-6(5)8(11)9(7,12)13/h1-4,12-13H
|
| Chemical Name |
2,2-dihydroxyindene-1,3-dione
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~280.68 mM)
H2O : ~25 mg/mL (~140.34 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (14.03 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.03 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 25 mg/mL (140.34 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.6136 mL | 28.0678 mL | 56.1356 mL | |
| 5 mM | 1.1227 mL | 5.6136 mL | 11.2271 mL | |
| 10 mM | 0.5614 mL | 2.8068 mL | 5.6136 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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