| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
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Nicotinamide Riboside Chloride (N-Ribosylnicotinamide chloride; Nicotinamide-beta-riboside chloride), a pyridine-nucleoside form of vitamin B3, is an orally bioactive precursor to NAD+ and a source of
vitamin B3. It can increase NAD[+] levels and activate SIRT1 and SIRT3.
| ln Vitro |
Nicotinamide riboside Chloride (0.5 nM; 24 hours) decreases Ndufa9 and SOD2 acetylation [1]. In C2C12, Hepa1.6, and HEK293 cells, nicotinamide riboside chloride raises intracellular and mitochondrial NAD+ levels in a concentration-dependent manner within the concentration range of 1-1000 μM [1]. Innate immunity against the coronavirus (CoV), which causes COVID-19, is supported by chlorinated nicotinamide riboside, which also increases NAD and restores antiviral poly(ADP-ribose) polymerase (PARP) function [3].
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|---|---|
| ln Vivo |
Nicotinamide riboside chloride oral supplementation (400 mg/kg/day) for 16 weeks enhances intracellular and plasma NAD+ levels in a tissue-specific manner [1].
|
| Cell Assay |
Western Blot Analysis[1]
Cell Types: HEK293T Cell Tested Concentrations: 0.5 nM Incubation Duration: 24 hrs (hours) Experimental Results: diminished acetylation status of Ndufa9 and SOD2. |
| Animal Protocol |
Animal/Disease Models: 10weeks old C57Bl/6J mice [1]
Doses: 400 mg/kg Route of Administration: PO; daily; continued for 16 weeks Experimental Results: Plasma and intracellular NAD+ content increased in a tissue-specific manner. |
| References |
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| Additional Infomation |
Nicotinamide riboside chloride is a N-glycosyl compound.
|
| Molecular Formula |
C11H15CLN2O5
|
|---|---|
| Molecular Weight |
290.7
|
| Exact Mass |
290.067
|
| CAS # |
23111-00-4
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| Related CAS # |
Nicotinamide riboside;1341-23-7;Nicotinamide riboside tartrate;2415657-86-0;Nicotinamide riboside malate;2415659-01-5
|
| PubChem CID |
90480033
|
| Appearance |
White to yellow solid powder
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
19
|
| Complexity |
314
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
C1=CC(=C[N+](=C1)[C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)O)C(=O)N.[Cl-]
|
| InChi Key |
YABIFCKURFRPPO-IVOJBTPCSA-N
|
| InChi Code |
InChI=1S/C11H14N2O5.ClH/c12-10(17)6-2-1-3-13(4-6)11-9(16)8(15)7(5-14)18-11;/h1-4,7-9,11,14-16H,5H2,(H-,12,17);1H/t7-,8-,9-,11-;/m1./s1
|
| Chemical Name |
1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]pyridin-1-ium-3-carboxamide;chloride
|
| Synonyms |
N-Ribosylnicotinamide chlorideNicotinamide-beta-riboside chloride Nicotinamide ribonucleoside chloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~125 mg/mL (~430.00 mM)
DMSO : ~50 mg/mL (~172.00 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 25 mg/mL (86.00 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4400 mL | 17.1999 mL | 34.3997 mL | |
| 5 mM | 0.6880 mL | 3.4400 mL | 6.8799 mL | |
| 10 mM | 0.3440 mL | 1.7200 mL | 3.4400 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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