| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
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| ln Vitro |
Without causing considerable cytotoxicity, neuropathiazol (5–15 μM; 5-hour exposure) significantly reduces cell growth [1]. Neuropathiazol (10 μM; 1-4 days) increases the marker for neuronal cells, NeuroD1, and downregulates the marker for neural progenitor cells, Sox2 [1]. Additionally, neuropathiazol prevents the differentiation of astrocytes produced by BMP2 and LIF [1]. Multipotent adult hippocampus neural progenitor cells undergo neuronal development when exposed to neuropathiazol [1]. In a dose-dependent manner, neuropathiazol can competitively suppress the synthesis of LIF/BMP2/FBS astrocytes [1].
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| Cell Assay |
Cell proliferation assay[1]
Cell Types: HCN Cell Tested Concentrations: 5 μM, 10 μM, 15 μM Incubation Duration: 5 hrs (hours) Experimental Results: HCN neural progenitor cell proliferation was inhibited. RT-PCR[1] Cell Types: HCN Cell Tested Concentrations: 10 μM Incubation Duration: 1 day, 4 days Experimental Results: Sox2 down-regulated, NeuroD1 up-regulated. |
| References |
[1]. Warashina M, et al. A synthetic small molecule that induces neuronal differentiation of adult hippocampal neural progenitor cells. Angew Chem Int Ed Engl. 2006 Jan 16;45(4):591-3.
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| Molecular Formula |
C19H18N2O2S
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|---|---|
| Molecular Weight |
338.42342
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| Exact Mass |
338.108
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| CAS # |
880090-88-0
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| PubChem CID |
11667240
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
517.1±56.0 °C at 760 mmHg
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| Flash Point |
266.6±31.8 °C
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| Vapour Pressure |
0.0±1.3 mmHg at 25°C
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| Index of Refraction |
1.626
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| LogP |
5.74
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
24
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| Complexity |
406
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(C1C=CC(N(C)C2=CSC(C3C=CC=CC=3)=N2)=CC=1)OCC
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| InChi Key |
MGKYEWWFHSESJN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C19H18N2O2S/c1-3-23-19(22)15-9-11-16(12-10-15)21(2)17-13-24-18(20-17)14-7-5-4-6-8-14/h4-13H,3H2,1-2H3
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| Chemical Name |
ethyl 4-[methyl-(2-phenyl-1,3-thiazol-4-yl)amino]benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~33.33 mg/mL (~98.49 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.39 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.39 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9549 mL | 14.7745 mL | 29.5491 mL | |
| 5 mM | 0.5910 mL | 2.9549 mL | 5.9098 mL | |
| 10 mM | 0.2955 mL | 1.4775 mL | 2.9549 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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