| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
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| Other Sizes |
| Targets |
Neocuproine targets metal ions, particularly copper(I) ions (Cu⁺). It acts as a chelating ligand that forms stable complexes with metal ions. The methyl groups at the 2 and 9 positions provide steric hindrance that enhances selectivity for Cu⁺ over other metal ions. By chelating metal ions, Neocuproine is used in analytical chemistry for metal ion detection and quantification, and in redox studies for modulating metal-dependent reactions.
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|---|---|
| ln Vitro |
Neocupron is a biochemical reagent that can be utilized in life science research as an organic substance or biological material.
In vitro, Neocuproine is used as a chelating agent that forms stable complexes with metal ions, particularly copper. It has enhanced selectivity and steric hindrance for complexing copper(I) ions. The compound is used in coordination chemistry and analytical applications. It is only slightly soluble in cold water but fully soluble in other liquids such as ethanol. The compound is a well-characterized chelating agent. |
| ln Vivo |
Neocuproine does not have pharmacological activity and is not used as a therapeutic agent. It is a chemical tool for coordination chemistry, analytical chemistry, and redox studies. In vivo applications are limited. The compound is used in research applications as a chelating agent and redox modulator. It is not intended for human or animal therapeutic use.
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| Enzyme Assay |
In vitro metal chelation assays for Neocuproine typically involve spectrophotometric measurement of metal complexes. The compound is dissolved in ethanol or DMSO and diluted in appropriate buffer. Metal ions (e.g., Cu²⁺, Cu⁺, Fe²⁺) are added at varying concentrations. Complex formation is monitored by UV-Vis spectroscopy, fluorescence, or colorimetric methods. Stoichiometry and stability constants are determined. Competitive binding assays may be performed with other metal chelators. Controls include metal ions alone and chelator alone.
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| Cell Assay |
For cell-based studies, Neocuproine is used to modulate copper availability in cell culture. Cells are cultured in appropriate medium and treated with Neocuproine at various concentrations (1-100 μM) for 24-72 hours. Copper-dependent cellular processes (e.g., oxidative stress, angiogenesis, mitochondrial function) are assessed. Cell viability is assessed by MTT or CellTiter-Glo. Copper levels in cells are measured by atomic absorption spectroscopy or ICP-MS. All treatments include vehicle controls and are performed in triplicate.
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| Animal Protocol |
In vivo studies with Neocuproine are not typical, as the compound is primarily used as a research chemical for metal chelation studies. The compound is not administered to animals for therapeutic purposes. It may be used in research applications for metal ion modulation in model organisms. Standard procedures for metal chelation research apply.
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| ADME/Pharmacokinetics |
Neocuproine (MW 208.26, C₁₄H₁₂N₂) is a phenanthroline-based chelating agent. It is a crystalline compound that is white to beige in color. It is only slightly soluble in cold water but fully soluble in ethanol. The compound is stable under standard storage conditions. It is typically stored at room temperature. It is for research purposes.
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| Toxicity/Toxicokinetics |
Neocuproine is a research chemical and not a therapeutic agent. It should be handled with standard laboratory safety precautions. The compound may cause irritation upon contact. Toxicity data are limited. The compound is for research use only and not intended for human or animal therapeutic applications. Standard safety precautions for chemical handling apply.
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| Additional Infomation |
Neocuproine is a phenanthrene-coral compound, specifically a 1,10-phenanthrene-coral compound with two methyl substituents at positions 2 and 9. It functions as both a chelating agent and a copper chelating agent.
Neocuproine (2,9-dimethyl-1,10-phenanthroline) is a research-grade chelating ligand for coordination chemistry, analytical applications, and redox studies. Its primary applications include metal chelation, analytical chemistry, and redox research. The compound is not a drug and has no clinical applications. It is commercially available from various chemical suppliers for research purposes only. Its mechanism involves selective chelation of copper(I) ions. |
| Molecular Formula |
C14H12N2
|
|---|---|
| Molecular Weight |
208.264
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| Exact Mass |
208.1
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| CAS # |
484-11-7
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| Related CAS # |
41066-08-4 (hydrochloride);7296-20-0 (mono-hydrochloride)
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| PubChem CID |
65237
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
367.4±37.0 °C at 760 mmHg
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| Melting Point |
159-164 °C
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| Flash Point |
159.7±17.8 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.693
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| LogP |
2.7
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
0
|
| Heavy Atom Count |
16
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| Complexity |
227
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| Defined Atom Stereocenter Count |
0
|
| InChi Key |
IYRGXJIJGHOCFS-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C14H12N2/c1-9-3-5-11-7-8-12-6-4-10(2)16-14(12)13(11)15-9/h3-8H,1-2H3
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| Chemical Name |
2,9-dimethyl-1,10-phenanthroline
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| Synonyms |
Neocuproin; Neocuproine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~240.08 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (12.00 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (12.00 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (12.00 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.8017 mL | 24.0085 mL | 48.0169 mL | |
| 5 mM | 0.9603 mL | 4.8017 mL | 9.6034 mL | |
| 10 mM | 0.4802 mL | 2.4008 mL | 4.8017 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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