| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| ln Vitro |
ALDH1A1, hALDH1A3, and hALDH2 are all inhibited by NCT-506, with IC50 values of 0.007±0.001, 16.4±3.99, and 21.5 μM, respectively[1]. With an EC50 of 45.6 μM, NCT-506 (100, 10, 1, 0.1 μM, 6 days) dramatically decreased cell viability in OV-90 cells. NCT-506 inhibited MIA PaCa-2, OV-90, and HT-29 cells with IC50 values of 0.077±0.040, 0.161±0.038, and 0.048±0.022 μM, respectively, in experiments using aldeflu cells [1]. The IC50 values for NCT-506 in combination with paclitaxel were 1202, 924, 870, 411, 102, and 31.8 nM, respectively, at SKOV-506 doses of 0 (DMSO), 1, 3, 10, 20, and 30 μM. correspondingly, 3 TR cells[1].
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| Cell Assay |
Cell viability assay [1]
Cell Types: OV-90 and SKOV-3-TR Cell Tested Concentrations: 100, 10, 1, 0.1 μM Incubation Duration: 6 days for OV-90 cells; 4 days for SKOV-3-TR cells Experimental Results: OV Cell viability was Dramatically diminished in -90 cells, with an EC50 of 45.6 μM. Combination treatment (paclitaxel concentration of 100 nM) diminished cell viability in SKOV-3-TR cells with an EC50 of 11.2 μM. |
| References |
| Molecular Formula |
C25H23FN4O3S
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|---|---|
| Molecular Weight |
478.538527727127
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| Exact Mass |
478.147
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| CAS # |
2231098-99-8
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| PubChem CID |
137628670
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| Appearance |
White to off-white solid powder
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| LogP |
2.4
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
34
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| Complexity |
924
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S(C)(N1CCN(C(C2C=NC3C=CC(=CC=3C=2C2C=CC(=CC=2)C2(C#N)CC2)F)=O)CC1)(=O)=O
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| InChi Key |
WIXZFNIBMHEUJH-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C25H23FN4O3S/c1-34(32,33)30-12-10-29(11-13-30)24(31)21-15-28-22-7-6-19(26)14-20(22)23(21)17-2-4-18(5-3-17)25(16-27)8-9-25/h2-7,14-15H,8-13H2,1H3
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| Chemical Name |
1-[4-[6-fluoro-3-(4-methylsulfonylpiperazine-1-carbonyl)quinolin-4-yl]phenyl]cyclopropane-1-carbonitrile
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~33.33 mg/mL (~69.65 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.22 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.22 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0897 mL | 10.4484 mL | 20.8969 mL | |
| 5 mM | 0.4179 mL | 2.0897 mL | 4.1794 mL | |
| 10 mM | 0.2090 mL | 1.0448 mL | 2.0897 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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