| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
| Other Sizes |
| ln Vitro |
As the fraction of organic phase increases, the retention time of NBD-F remains rather steady, but the retention time of the derivatized products changes. The pH value of the mobile phase impacts the separation of NBD-F and derivative products [1]. NBD-F is a fluorescent derivatization reagent originally designed for amino acid analysis and has recently been extended to the analysis of additional amino acid derivatives such as N-methyl-D-aspartate and glutathione. The usage of NBD-F seems to have numerous advantages, especially the simplicity of the derivatization procedure and the high stability of its derivatives [2].
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| Enzyme Assay |
- Derivatization of amino acid neurotransmitters: Rats were sacrificed, and hippocampi were isolated, homogenized, and centrifuged to obtain the supernatant. An appropriate amount of supernatant was mixed with borate buffer to adjust pH to 9.0, followed by the addition of excess NBD-F solution dissolved in an organic solvent. The mixture was incubated in a 60°C water bath for 40 minutes to allow derivatization between NBD-F and the amino groups of amino acid neurotransmitters. Acetic acid was added to terminate the reaction, and the mixture was centrifuged. The resulting supernatant was injected into an HPLC system for separation and detection by UV detector [1]
- Derivatization of free sphingoid bases: Lipids were extracted from samples using organic solvents, and the free sphingoid base fraction was isolated. An appropriate amount of this fraction was dissolved in methanol, then NBD-F solution and triethylamine (as a catalyst) were added. The mixture was incubated at 50°C for 30 minutes to promote the reaction between NBD-F and the amino groups of free sphingoid bases. Hydrochloric acid was added to terminate the reaction, and the derivatized products were purified by solid-phase extraction or liquid-liquid extraction to remove excess reagents and impurities. The purified samples were injected into an LC-MS/MS system for qualitative identification and quantitative analysis [2] |
| References |
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| Additional Infomation |
NBD-F (4-fluoro-7-nitrobenzofuran) is a fluorescent derivatizing reagent that can specifically react with primary and secondary amines to generate stable fluorescent derivatives[1][2]
- In relevant literature, NBD-F is mainly used to derivatize amino acid neurotransmitters and free sphingosine bases, thereby improving the detection sensitivity and quantitative accuracy of these analytes by HPLC-UV or LC-MS/MS[1][2] - The derivatization reaction of NBD-F with amines shows high efficiency under mild conditions (medium temperature and specific pH value), and the resulting derivatives have excellent chromatographic separation performance and high detection sensitivity[1][2] |
| Molecular Formula |
C6H2FN3O3
|
|---|---|
| Molecular Weight |
183.0968
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| Exact Mass |
183.008
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| CAS # |
29270-56-2
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| PubChem CID |
122123
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.7±0.1 g/cm3
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| Boiling Point |
308.6±45.0 °C at 760 mmHg
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| Melting Point |
52-54 °C(lit.)
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| Flash Point |
140.4±28.7 °C
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| Vapour Pressure |
0.0±0.6 mmHg at 25°C
|
| Index of Refraction |
1.638
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| LogP |
1.27
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
0
|
| Heavy Atom Count |
13
|
| Complexity |
222
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
PGZIDERTDJHJFY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H2FN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H
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| Chemical Name |
4-fluoro-7-nitro-2,1,3-benzoxadiazole
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~546.15 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (13.65 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.4615 mL | 27.3075 mL | 54.6150 mL | |
| 5 mM | 1.0923 mL | 5.4615 mL | 10.9230 mL | |
| 10 mM | 0.5461 mL | 2.7307 mL | 5.4615 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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