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Other Sizes |
ln Vivo |
By influencing inflammation, NGF, and VEGF Mouse conjunctivitis, naphazine nitrate (0.2 mg/kg, 10 µl per eye; IP once) decreases conjunctival vascular hyperpermeability in mice caused by histamines or antigens[1].
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Animal Protocol |
Animal/Disease Models: Female wild-type balb/c (Bagg ALBino) mouse (4-5 weeks, 18±2g, n=8/group, using histamine or antigen (ovalbumin) to establish allergic conjunctivitis mouse model) [1]
Doses: 0.2mg/mL, 10 µl per eye Route of Administration: intraperitoneal (ip) injection, once Experimental Results: Dramatically inhibited conjunctival dye leakage in mice with histamine- or antigen-induced conjunctival vascular hyperpermeability. Reduces inflammatory responses and levels of IL-1β, IL-6, IFN-γ, and IL-4. Reduces the levels of IgE, GMCSF, NGF and VEGF in mice with antigen-induced conjunctival vascular hyperpermeability. |
References |
[1]. Quan L, et, al. Treatment with olopatadine and naphazoline hydrochloride reduces allergic conjunctivitis in mice through alterations in inflammation, NGF and VEGF. Mol Med Rep. 2016 Apr;13(4):3319-25.
[2]. Yamaguchi I, et, al. Central and peripheral adrenergic mechanisms regulating gastric secretion in the rat. J Pharmacol Exp Ther. 1977 Oct;203(1):125-31. |
Molecular Formula |
C14H15N3O3
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Molecular Weight |
273.29
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Exact Mass |
273.1113
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CAS # |
5144-52-5
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Related CAS # |
Naphazoline hydrochloride;550-99-2;Naphazoline;835-31-4
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SMILES |
O[N+]([O-])=O.C1(CC2=C3C=CC=CC3=CC=C2)=NCCN1
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Synonyms |
Naphazoline nitrate Clera Benil
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~365.91 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.15 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.15 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (9.15 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.6591 mL | 18.2956 mL | 36.5912 mL | |
5 mM | 0.7318 mL | 3.6591 mL | 7.3182 mL | |
10 mM | 0.3659 mL | 1.8296 mL | 3.6591 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.