| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
- Acetylcholinesterase (AChE)：N-p-trans-Coumaroyltyramine acts as an inhibitor of AChE with an IC₅₀ value of 38.5 μM [2]
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|---|---|
| ln Vitro |
1. AChE inhibitory activity:
- N-p-trans-Coumaroyltyramine showed dose-dependent inhibitory activity against electric eel AChE in vitro [2] - At a concentration of 10 μM, N-p-trans-Coumaroyltyramine inhibited AChE activity by 18.2%; at 50 μM, the inhibition rate increased to 56.7%; the IC₅₀ value was determined as 38.5 μM via dose-response curve fitting [2] - The inhibitory activity was compared to the positive control (physostigmine, IC₅₀ = 0.02 μM), showing weaker but significant AChE inhibition [2] |
| Enzyme Assay |
1. Acetylcholinesterase (AChE) activity inhibition assay:
- Reaction system composition: Total volume 3 ml, containing 50 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 0.02 M MgCl₂, 0.5 mM 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB), 0.8 mM acetylthiocholine iodide (ATCI, AChE substrate), and different concentrations of N-p-trans-Coumaroyltyramine (5-100 μM) [2] - Incubation and reaction initiation: The mixture (excluding AChE) was pre-incubated at 37°C for 5 minutes; 0.1 U of electric eel AChE was added to initiate the reaction [2] - Activity detection: The enzyme-catalyzed reaction produces thiocholine, which reacts with DTNB to form a yellow product; the absorbance was measured at 412 nm every 30 seconds for 5 minutes using a spectrophotometer [2] - Inhibition rate calculation: AChE activity was calculated based on the initial reaction rate (slope of absorbance vs. time); the inhibition rate (%) = [(Activity of control - Activity of treated group) / Activity of control] × 100 [2] - IC₅₀ determination: The IC₅₀ value was derived from plotting inhibition rate against N-p-trans-Coumaroyltyramine concentration and fitting with a logistic dose-response model [2] |
| References | |
| Additional Infomation |
Trans-N-p-coumaryltyramine is a hydroxycinnamic acid and a metabolite. It has been reported in Celtis bungeana, Tinospora sinensis, and other organisms with relevant data. See also: aerial parts of Cannabis sativa subsp. indica; leaf parts of Ipomoea aquatica.
1. Source and isolation of N-p-trans-coumaryltyramine: - - N-p-trans-coumaryltyramine was isolated from the branches of Celtis chinensis (Ulmaceae)[2] - - Overview of the isolation process: dried Celtis chinensis branches were extracted with 80% methanol aqueous solution; the extract was extracted with ethyl acetate, and the ethyl acetate fraction was separated by silica gel column chromatography (chloroform-methanol gradient elution) and reversed-phase high performance liquid chromatography (C18 column, mobile phase methanol-water) to obtain pure Np-trans-coumaryltyramine[2] 2. Chemical structure characterization: - The structure of Np-trans-coumaryltyramine was confirmed by spectroscopic methods ¹H-NMR, ¹³C-NMR and mass spectrometry (EI-MS)[2] - Structural features: It is composed of a trans-coumaryl group (trans-p-hydroxycinnamoyl group) linked to the amino group of tyramine (4-hydroxy-β-phenylethylamine) [2] 3. Biological significance: -Acetylcholinesterase inhibitors are used to treat Alzheimer's disease (AD) by increasing acetylcholine levels. Brain content; The acetylcholinesterase inhibitory activity of Np-trans-coumaryltyramine suggests its potential as a lead compound for Alzheimer's disease-related research [2] |
| Molecular Formula |
C17H17NO3
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|---|---|
| Molecular Weight |
283.3218
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| Exact Mass |
283.12
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| CAS # |
36417-86-4
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| PubChem CID |
5372945
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| Appearance |
White to light yellow solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
586.5±50.0 °C at 760 mmHg
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| Melting Point |
260 - 261 °C
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| Flash Point |
308.5±30.1 °C
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| Vapour Pressure |
0.0±1.7 mmHg at 25°C
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| Index of Refraction |
1.655
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| LogP |
2.24
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
21
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| Complexity |
340
|
| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC(=CC=C1CCNC(=O)/C=C/C2=CC=C(C=C2)O)O
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| InChi Key |
RXGUTQNKCXHALN-BJMVGYQFSA-N
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| InChi Code |
InChI=1S/C17H17NO3/c19-15-6-1-13(2-7-15)5-10-17(21)18-12-11-14-3-8-16(20)9-4-14/h1-10,19-20H,11-12H2,(H,18,21)/b10-5+
|
| Chemical Name |
(E)-3-(4-hydroxyphenyl)-N-[2-(4-hydroxyphenyl)ethyl]prop-2-enamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~352.96 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.82 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.82 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.82 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5296 mL | 17.6479 mL | 35.2958 mL | |
| 5 mM | 0.7059 mL | 3.5296 mL | 7.0592 mL | |
| 10 mM | 0.3530 mL | 1.7648 mL | 3.5296 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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