| Size | Price | Stock | Qty |
|---|---|---|---|
| 25g |
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| Other Sizes |
| Targets |
N-Methylsarcosine targets multiple pathways as an endogenous metabolite and amino acid derivative. It acts as an iGluR (ionotropic glutamate receptor) modulator and is involved in amino acid metabolism. The compound exhibits antiviral activity against SARS-CoV-2 by inhibiting virus-induced cytotoxicity. As a methyl donor and metabolic intermediate, it participates in various biochemical pathways including homocysteine metabolism, where it is a byproduct. It also functions as an immunostimulant and athletic performance enhancer.
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| ln Vitro |
In vitro, N-Methylsarcosine exhibits antiviral activity against SARS-CoV-2, as evaluated by its capacity to inhibit SARS-CoV-2-induced cytotoxicity in VERO-6 cells. The compound is an amino acid building block for protein synthesis and is found in small amounts in the body. It has been studied for its immunostimulatory properties and its role as an endogenous metabolite. The compound's biological activity has been characterized in various cell-based assays.
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| ln Vivo |
In vivo, N-Methylsarcosine (dimethylglycine) is used as an athletic performance enhancer and immunostimulant. It has been investigated for therapeutic applications in autism, epilepsy, and mitochondrial disease. The compound is a byproduct of homocysteine metabolism and is found in cells of all plants and animals. It can be obtained in small amounts from grains and meat in the diet. Its reputed ability to increase endurance and reduce fatigue has made it popular as a dietary supplement.
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| Enzyme Assay |
For non-cell enzyme/receptor binding assays, N-Methylsarcosine can be studied for its interactions with iGluR and other receptors using membrane preparations. Radioligand binding assays or fluorescence-based assays can be employed to assess receptor binding affinity. The compound's metabolic role can be studied using enzyme activity assays in cell-free systems. Its antioxidant and immunomodulatory properties can be assessed using cell-free biochemical assays.
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| Cell Assay |
For in vitro cell-based assays, N-Methylsarcosine activity is assessed in various cell lines including VERO-6 cells for antiviral activity. Cells are treated with the compound, and cytotoxicity is measured to assess antiviral efficacy. Immunostimulatory effects are evaluated in immune cell cultures by measuring cytokine production and immune cell activation. The compound's effects on cellular metabolism and protein synthesis can also be studied in cell culture models.
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| Animal Protocol |
For in vivo animal experiments, N-Methylsarcosine is typically administered orally or intraperitoneally to animal models. Studies have investigated its effects on exercise performance, immune function, and metabolic parameters. The compound's therapeutic potential in autism, epilepsy, and mitochondrial disease has been evaluated in animal models. Pharmacokinetic studies assess the absorption, distribution, metabolism, and elimination of the compound in vivo.
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| ADME/Pharmacokinetics |
N-Methylsarcosine has a molecular weight of 103.12 g/mol and molecular formula C₄H₉NO₂. It is also known as dimethylglycine (DMG) or N,N-dimethylglycine. The compound is soluble in water at 90 mg/mL and appears as a white solid. It should be stored as powder at -20°C for up to 3 years or at 4°C for up to 2 years. In solvent, it can be stored at -80°C for 6 months or at -20°C for 1 month.
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| Toxicity/Toxicokinetics |
N-Methylsarcosine (dimethylglycine) is considered safe at typical dietary supplement doses. It is an endogenous metabolite found naturally in the body and in foods. No significant toxicity has been reported at physiological concentrations. The compound is used as a dietary supplement for athletic performance enhancement and immune support. High doses may cause mild gastrointestinal effects. It is for research use and dietary supplement applications, not as a pharmaceutical drug.
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| Additional Infomation |
N,N-Dimethylglycine is an N-methylglycine, specifically glycine with two N-methyl substituents. It is a human metabolite, a Daphnia magna metabolite, and a mouse metabolite. It is an N-methyl amino acid belonging to the N-methylglycine family. It is a zwitterionic tautomer of N,N-dimethylglycine. N,N-Dimethylglycine is a metabolite found or produced in Escherichia coli (strains K12 and MG1655). N,N-Dimethylglycine has been reported in Paris fargesii, humans (Homo sapiens), and other organisms with relevant data. See also: N,N-Dimethylglycine hydrochloride (active fraction)... See more...
N-Methylsarcosine (N,N-dimethylglycine, DMG) is an endogenous amino acid derivative found in small amounts in all plant and animal cells. It is a byproduct of homocysteine metabolism and can be obtained from dietary sources such as grains and meat. The compound is used as an athletic performance enhancer and immunostimulant, and has been studied for treating autism, epilepsy, and mitochondrial disease. It also exhibits antiviral activity against SARS-CoV-2. The compound is not an approved pharmaceutical drug but is available as a dietary supplement. |
| Molecular Formula |
C4H9NO2
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|---|---|
| Molecular Weight |
103.1198
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| Exact Mass |
103.063
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| CAS # |
1118-68-9
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| Related CAS # |
17647-86-8 (mono-potassium salt);18319-88-5 (hydrochloride salt);2491-06-7 (mono-hydrochloride)
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| PubChem CID |
673
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| Appearance |
White to off-white solid powder
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
175.2±23.0 °C at 760 mmHg
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| Melting Point |
178-182 °C(lit.)
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| Flash Point |
59.8±22.6 °C
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| Vapour Pressure |
0.6±0.7 mmHg at 25°C
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| Index of Refraction |
1.449
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| LogP |
-0.3
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
7
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| Complexity |
70.1
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| Defined Atom Stereocenter Count |
0
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~969.74 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (969.74 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.6974 mL | 48.4872 mL | 96.9744 mL | |
| 5 mM | 1.9395 mL | 9.6974 mL | 19.3949 mL | |
| 10 mM | 0.9697 mL | 4.8487 mL | 9.6974 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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