| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
N-Butylthiophosphoric triamide targets urease, an enzyme that catalyzes the hydrolysis of urea into ammonia and carbon dioxide. By inhibiting urease, NBPT reduces the conversion of urea to NH3 gas, thereby enhancing nitrogen use efficiency in fertilizers.
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|---|---|
| ln Vitro |
In vitro, N-Butylthiophosphoric triamide is a potent urease inhibitor. It inhibits the activity of the urease enzyme, preventing the hydrolysis of urea. However, specific IC50 values and detailed in vitro activity data are not extensively reported.
|
| ln Vivo |
In vivo, N-Butylthiophosphoric triamide is used in agricultural settings to enhance nitrogen use efficiency in fertilizers. By inhibiting urease, it reduces the loss of nitrogen through ammonia volatilization.
|
| Enzyme Assay |
In vitro enzyme assays for N-Butylthiophosphoric triamide involve urease activity assays. The compound is incubated with urease and urea, and the production of ammonia is measured. The inhibition of urease activity is assessed to determine the compound's potency.
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| Cell Assay |
There are no specific in vitro cellular assays for N-Butylthiophosphoric triamide, as it is primarily used as an agricultural chemical rather than a drug.
|
| Animal Protocol |
There are no specific in vivo animal studies for N-Butylthiophosphoric triamide, as it is primarily used in agricultural settings.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of N-Butylthiophosphoric triamide are not applicable, as it is an agricultural chemical rather than a drug. It has a molecular weight of 167.21.
|
| Toxicity/Toxicokinetics |
The toxicity profile of N-Butylthiophosphoric triamide is not extensively reported. As an agricultural chemical, it is not intended for human therapeutic use. No specific toxicity data are detailed.
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| Additional Infomation |
N-Butylthiophosphoric triamide (CAS 94317-64-3, NBPT) is a potent urease inhibitor used in agriculture to enhance nitrogen use efficiency in fertilizers. It has a molecular formula of C4H14N3PS and a molecular weight of 167.21. The compound is available for research purposes only.
|
| Molecular Formula |
C4H14N3PS
|
|---|---|
| Molecular Weight |
167.2128
|
| Exact Mass |
167.064
|
| CAS # |
94317-64-3
|
| PubChem CID |
93502
|
| Appearance |
White to off-white solid powder
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
277.4±23.0 °C at 760 mmHg
|
| Melting Point |
58-60°C
|
| Flash Point |
121.6±22.6 °C
|
| Vapour Pressure |
0.0±0.6 mmHg at 25°C
|
| Index of Refraction |
1.549
|
| LogP |
-0.77
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
9
|
| Complexity |
110
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
S=P(NCCCC)(N)N
|
| InChi Key |
HEPPIYNOUFWEPP-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C4H14N3PS/c1-2-3-4-7-8(5,6)9/h2-4H2,1H3,(H5,5,6,7,9)
|
| Chemical Name |
N-diaminophosphinothioylbutan-1-amine
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~598.05 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (14.95 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.95 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (14.95 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.9805 mL | 29.9025 mL | 59.8050 mL | |
| 5 mM | 1.1961 mL | 5.9805 mL | 11.9610 mL | |
| 10 mM | 0.5981 mL | 2.9903 mL | 5.9805 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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