| Size | Price | Stock | Qty |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
N-Acetylornithine is an intermediate in the arginine biosynthetic pathway. It is formed from L-glutamate and subsequently converted to ornithine. The compound is essential for nitrogen metabolism and amino acid homeostasis in many organisms, particularly bacteria and plants. It has a role as a mouse metabolite, a Saccharomyces cerevisiae metabolite, an Escherichia coli metabolite, and a human metabolite.
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| ln Vitro |
In vitro, N-Acetylornithine is used as a substrate to study the enzymes involved in arginine biosynthesis. It is a minor component of deproteinized blood plasma of human blood. The compound is used in biochemical and metabolic studies focused on arginine biosynthesis, enzymatic regulation, and nitrogen assimilation pathways. Its role as a metabolic intermediate has been characterized in various in vitro systems.
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| ln Vivo |
In vivo, N-Acetylornithine is a human metabolite found in blood plasma. It is formed during arginine biosynthesis and is involved in nitrogen metabolism. The compound is a metabolite in E. coli, S. cerevisiae, and mice. Its levels may reflect arginine biosynthesis activity. Further in vivo studies are needed to fully characterize its physiological roles.
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| Enzyme Assay |
In vitro enzyme assays for N-Acetylornithine involve measuring the activity of enzymes in the arginine biosynthetic pathway. Enzymes such as ornithine acetyltransferase are assessed by monitoring the conversion of N-acetylornithine to ornithine. The compound serves as a substrate in these assays. For metabolic studies, N-acetylornithine levels are measured by HPLC or LC-MS in biological samples. Assays are performed in appropriate buffer systems with positive controls.
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| Cell Assay |
In vitro cell-based assays for N-Acetylornithine are conducted in various cell lines to study arginine biosynthesis and nitrogen metabolism. Cells are cultured in appropriate media and treated with the compound at varying concentrations. Metabolic flux is assessed by measuring arginine and ornithine levels. Cell viability is assessed by standard assays. Experiments are performed in triplicate with appropriate positive and negative controls.
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| Animal Protocol |
N-Acetylornithine in vivo studies are conducted in animal models for metabolic research. Animals are administered the compound via oral administration or injection. Amino acid and metabolite levels in blood and tissues are measured by HPLC or LC-MS. Nitrogen metabolism and arginine biosynthesis are assessed. Animals are monitored for clinical signs. Tissues and blood samples are collected for biochemical analysis at study endpoints. Studies are conducted in accordance with institutional animal care guidelines.
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| ADME/Pharmacokinetics |
N-Acetylornithine (MW 174.20 g/mol, C7H14N2O3) has a purity of ≥95%. It is an N(2)-acyl-L-ornithine where the acyl group is specified as acetyl. The compound is soluble in water and other appropriate solvents. It is stable under recommended storage conditions. N-Acetylornithine is a human metabolite and a minor component of blood plasma. Pharmacokinetic parameters such as half-life and bioavailability would be determined in species-specific studies.
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| Toxicity/Toxicokinetics |
N-Acetylornithine is generally well-tolerated as an endogenous metabolite. The compound is a human metabolite and a key intermediate in arginine biosynthesis. No significant adverse effects have been reported in the available literature at research-use concentrations. The compound is intended for research use only. Standard laboratory safety practices should be employed when handling this compound. Comprehensive toxicological evaluation would be required for therapeutic development.
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| Additional Infomation |
N(2)-Acetyl-L-ornithine is an N(2)-acyl-L-ornithine, where the acyl group is specifically designated as an acetyl group. It is a human metabolite, an Escherichia coli metabolite, a Saccharomyces cerevisiae metabolite, and a mouse metabolite. It is both acetyl-L-ornithine and N2-acyl-L-ornithine. It is a zwitterionic tautomer of N(2)-acetyl-L-ornithine. N-Acetylornithine is a metabolite found or produced in Escherichia coli (K12 strain, MG1655 strain). Acetylornithine has also been reported in Arabidopsis thaliana, humans, and other organisms with relevant data.
N-Acetylornithine (N-Acetyl-L-ornithine) is a key metabolic intermediate in the enzymatic biosynthesis of L-arginine from L-glutamate. It is formed during the acetylated branch of the arginine pathway and converted to ornithine through deacetylation. The compound is a human, E. coli, S. cerevisiae, and mouse metabolite. It is widely used in biochemical and metabolic studies of arginine biosynthesis, enzymatic regulation, and nitrogen assimilation. Its molecular formula is C7H14N2O3 with a molecular weight of 174.20 g/mol.All applications are limited to non-human research use. |
| Molecular Formula |
C7H14N2O3
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|---|---|
| Molecular Weight |
174.1977
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| Exact Mass |
174.1
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| CAS # |
6205-08-9
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| Related CAS # |
N-Acetylornithine-d2
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| PubChem CID |
439232
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| Appearance |
White to off-white solid powder
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| Density |
1.171g/cm3
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| Boiling Point |
436.2ºC at 760 mmHg
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| Flash Point |
217.6ºC
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| Index of Refraction |
1.492
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| LogP |
0.405
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
12
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| Complexity |
170
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC(=O)N[C@@H](CCCN)C(=O)O
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| InChi Key |
JRLGPAXAGHMNOL-LURJTMIESA-N
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| InChi Code |
InChI=1S/C7H14N2O3/c1-5(10)9-6(7(11)12)3-2-4-8/h6H,2-4,8H2,1H3,(H,9,10)(H,11,12)/t6-/m0/s1
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| Chemical Name |
(2S)-2-acetamido-5-aminopentanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~574.05 mM)
DMSO : ~1.96 mg/mL (~11.25 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (574.05 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.7405 mL | 28.7026 mL | 57.4053 mL | |
| 5 mM | 1.1481 mL | 5.7405 mL | 11.4811 mL | |
| 10 mM | 0.5741 mL | 2.8703 mL | 5.7405 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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