| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
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| ln Vitro |
Advice (The procedure we suggest is as follows. This protocol should be adjusted to suit your unique requirements; it merely offers guidelines. 1. Setting up the experimental and control wells: Parallel negative, positive, and experimental wells should make up the experiment, in that order. Test wells: Pour in 200 μL of cell culture media with the appropriate amount of GSH effectors (e.g., 200 μM H2O2). 2. Let the plate sit in 5% CO2 at 37°C for the entire night. Depending on your standard procedure, incubation times can differ. 3. At a final concentration of 20–100 μM, add monochlorodimethane (mBCl) from the 1 mM working solution to the cells. Dimethyl sulfoxide (DMSO) was used to prepare stock solutions of mBCl (50 mM), which were then kept at -20°C. Prior to use, the stock solution was diluted in 0.1 M PBS buffer (pH 7.0). In the experiment, the final DMSO concentration is less than 0.2% (v/v). 4. For around two hours, incubate the cell suspension with mBCl at 25°C in the dark. 5. Centrifuge the dye at 700 X g for five minutes to remove it. Fill each well with 200 μL of Assay Buffer, then proceed with your chosen detection technique. 6. GSH detection inside cells: The fluorescence plate reader can read fluorescence straight from the culture plate and is appropriate for adherent cells. To record fluorescence (blue) at EX/EM = 380/465 ±20 nm, respectively, aliquot 200 μL of culture suspension into white opaque plates if employing suspension cells.
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|---|---|
| ADME/Pharmacokinetics |
Metabolism / Metabolites
Monochlorobimane has known human metabolites that include Bimane-glutathione. |
| References | |
| Additional Infomation |
Monochlorobimane is an organochlorine compound and a pyrazolopyrazole. It has a role as a fluorochrome.
|
| Molecular Formula |
C10H11N2O2CL
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|---|---|
| Molecular Weight |
226.65954
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| Exact Mass |
226.051
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| CAS # |
76421-73-3
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| PubChem CID |
114886
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.4g/cm3
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| Boiling Point |
314.9ºC at 760 mmHg
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| Melting Point |
135-136ºC(lit.)
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| Flash Point |
144.2ºC
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| Index of Refraction |
1.61
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| LogP |
0.861
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
15
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| Complexity |
437
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| Defined Atom Stereocenter Count |
0
|
| InChi Key |
SUIPVTCEECPFIB-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H11ClN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
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| Chemical Name |
7-(chloromethyl)-1,2,6-trimethylpyrazolo[1,2-a]pyrazole-3,5-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~220.59 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (11.03 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (11.03 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4119 mL | 22.0595 mL | 44.1189 mL | |
| 5 mM | 0.8824 mL | 4.4119 mL | 8.8238 mL | |
| 10 mM | 0.4412 mL | 2.2059 mL | 4.4119 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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