| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
In splenocytes from wt or LMP7-deficient mice, treatment with ML604440 (300 nM; overnight) had an impact on the surface expression of H-2Kb [2]. ML604440 treatment (300 nM; 24 hours) did not substantially stain human PBMC or mice splenocytes. ML604440 (300 nM; 3 days) had no influence on the overall quantity of CD4+ T cells that produce IL-17A [2].
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|---|---|
| ln Vivo |
LMP2 is stained in vivo by ML604440 treatment (ip; 10 mg/kg; once daily; 7 days) without causing appreciable alterations to the projection behind it [3].
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| Animal Protocol |
Animal/Disease Models: Male C57BL/6J mice (6-8 weeks old) were injected with anti-platelet monoclonal antibodies [3]
Doses: 10 mg/kg Route of Administration: intraperitoneal (ip) injection; 10 mg/kg; one time/day; 7-day Experimental Results: LMP2 is inhibited in vivo. There was no significant improvement in platelet counts after immunizing mice with a monoclonal rat anti-mouse CD41 platelet antibody. |
| References |
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| Molecular Formula |
C17H24BF3N2O4
|
|---|---|
| Molecular Weight |
388.189675331116
|
| Exact Mass |
388.178
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| CAS # |
1140517-08-3
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| PubChem CID |
57655545
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| Appearance |
White to yellow solid powder
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
27
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| Complexity |
527
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| Defined Atom Stereocenter Count |
1
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| SMILES |
FC(C1C=CC=CC=1C(NC(C)(C)C(N[C@H](B(O)O)CC(C)C)=O)=O)(F)F
|
| InChi Key |
IRZZVDHGNHGNAI-ZDUSSCGKSA-N
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| InChi Code |
InChI=1S/C17H24BF3N2O4/c1-10(2)9-13(18(26)27)22-15(25)16(3,4)23-14(24)11-7-5-6-8-12(11)17(19,20)21/h5-8,10,13,26-27H,9H2,1-4H3,(H,22,25)(H,23,24)/t13-/m0/s1
|
| Chemical Name |
[(1R)-3-methyl-1-[[2-methyl-2-[[2-(trifluoromethyl)benzoyl]amino]propanoyl]amino]butyl]boronic acid
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~257.61 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.44 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.44 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.44 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5761 mL | 12.8803 mL | 25.7606 mL | |
| 5 mM | 0.5152 mL | 2.5761 mL | 5.1521 mL | |
| 10 mM | 0.2576 mL | 1.2880 mL | 2.5761 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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