| Size | Price | |
|---|---|---|
| Other Sizes |
| ln Vitro |
It has been demonstrated that Nox1-dependent ROS generation is essential for cell signaling, cell proliferation, angiogenesis, exercise, and blood pressure regulation. In HT29 cells, ML171 significantly inhibits the formation of ROS (IC50=0.129 μM). Nox1-dependent ROS production in the HEK293 cell system is dependent on higher overexpression of Nox1, which can only be achieved by overexpressing Nox1 in response to ML171 treatment. Every ingredient is rebuilt. When ROS production is assessed using carboxy-H2-DCFDA staining with DPI as a positive control, ML171 efficiently inhibits it. In comparison to the parent drug, ML171 demonstrated greater effectiveness in inhibiting Nox1-dependent ROS generation in the HEK293-Nox1 recombinant cell system [1].
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|---|---|
| References | |
| Additional Infomation |
1-(10H-phenothiazin-2-yl)ethanone is a member of phenothiazines.
|
| Molecular Formula |
C14H11NOS
|
|---|---|
| Molecular Weight |
241.3082
|
| Exact Mass |
241.056
|
| CAS # |
6631-94-3
|
| PubChem CID |
81131
|
| Appearance |
Yellow to orange solid powder
|
| Density |
1.249 g/cm3
|
| Boiling Point |
455.7ºC at 760 mmHg
|
| Melting Point |
180-185 °C(lit.)
|
| Flash Point |
229.4ºC
|
| Index of Refraction |
1.653
|
| LogP |
4.235
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
17
|
| Complexity |
307
|
| Defined Atom Stereocenter Count |
0
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ≥ 64 mg/mL (~265.22 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.36 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.36 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1440 mL | 20.7202 mL | 41.4405 mL | |
| 5 mM | 0.8288 mL | 4.1440 mL | 8.2881 mL | |
| 10 mM | 0.4144 mL | 2.0720 mL | 4.1440 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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