| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
Mitoridine does not have a well-defined specific therapeutic target but is a naturally occurring pyrrolizidine alkaloid. As a pyrrolizidine alkaloid, it may interact with multiple cellular targets and exhibit various biological activities. Pyrrolizidine alkaloids are known for their hepatotoxicity and genotoxicity, which are mediated through metabolic activation to reactive pyrrole intermediates. The compound's mechanism of action involves metabolic activation and formation of DNA adducts.
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| ln Vitro |
Mitoridine is a pyrrolizidine alkaloid compound with potential biological activities. As a natural product, it is part of a class of compounds known for diverse biological activities including hepatotoxicity, genotoxicity, and anticancer effects. The compound's specific biological activities and potency values are not extensively documented in the public domain. It is used in natural product research.
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| ln Vivo |
In vivo activity data for Mitoridine are not extensively documented. As a pyrrolizidine alkaloid, it may have hepatotoxic and genotoxic effects in vivo. Pyrrolizidine alkaloids are metabolized in the liver to reactive pyrrole intermediates that can form DNA adducts and cause liver damage. The compound is not used as a therapeutic agent but is studied for its toxicological effects. Specific in vivo efficacy studies are not applicable.
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| Enzyme Assay |
The in vitro assay for Mitoridine typically involves testing its cytotoxic or genotoxic effects on cell lines. Cells are treated with varying concentrations of the compound (typically 0.1-100 µM) for 24-72 hours. Cell viability is assessed using MTT or other standard assays. Genotoxicity is assessed using comet assays or micronucleus assays. The compound is dissolved in DMSO and diluted in cell culture medium.
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| Cell Assay |
In vitro cellular assays for Mitoridine typically involve treating various cell lines (such as liver cells) with the compound at concentrations ranging from 0.1 to 100 µM for 24-72 hours. Cell viability is assessed using MTT, CCK-8, or CellTiter-Glo assays. Genotoxicity is assessed by comet assays or micronucleus assays. The compound is dissolved in DMSO as a stock solution and diluted in cell culture medium.
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| Animal Protocol |
In vivo animal studies for Mitoridine are typically conducted to assess its toxicological effects. The compound is administered to rodents via oral gavage or intraperitoneal injection. Liver function is assessed by measuring liver enzymes and histopathological examination. Genotoxicity is assessed by micronucleus assays or comet assays in blood or liver cells. The compound is not intended for therapeutic use.
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| ADME/Pharmacokinetics |
Mitoridine has a molecular weight of 302.37 g/mol and formula C18H22N2O2. It is a pyrrolizidine alkaloid. The compound is soluble in organic solvents such as DMSO and ethanol. Recommended storage is typically -20°C for powder. Detailed PK parameters such as half-life, Cmax, AUC, and bioavailability would require experimental determination.
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| Toxicity/Toxicokinetics |
Mitoridine is intended for research use only and is not approved for human therapeutic applications. As a pyrrolizidine alkaloid, it may have hepatotoxic and genotoxic effects. Appropriate safety precautions should be taken when handling. Standard toxicity assessments would include acute toxicity, genotoxicity, and repeated-dose toxicity studies.
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| References | |
| Additional Infomation |
Mitoridine (CAS 3911-19-1) is a natural pyrrolizidine alkaloid with a molecular weight of 302.37 g/mol and formula C18H22N2O2. Pyrrolizidine alkaloids are known for their hepatotoxicity and genotoxicity. Mitoridine is used in natural product research and toxicological studies. The compound is not approved for clinical use.
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| Molecular Formula |
C20H22N2O2
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|---|---|
| Molecular Weight |
322.40088
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| Exact Mass |
322.168
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| CAS # |
3911-19-1
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| PubChem CID |
101297641
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
522.1±50.0 °C at 760 mmHg
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| Melting Point |
322℃
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| Flash Point |
269.6±30.1 °C
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| Vapour Pressure |
0.0±1.4 mmHg at 25°C
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| Index of Refraction |
1.722
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| LogP |
2.05
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
24
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| Complexity |
660
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| Defined Atom Stereocenter Count |
4
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| SMILES |
C/C=C/1\CN2[C@H]3CC45[C@H](C2C[C@@H]1[C@@H]3C4=O)N(C6=C5C=CC=C6O)C
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| InChi Key |
VVLPFXAEVKKWTK-JTJLKIRNSA-N
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| InChi Code |
InChI=1S/C20H22N2O2/c1-3-10-9-22-13-7-11(10)16-14(22)8-20(19(16)24)12-5-4-6-15(23)17(12)21(2)18(13)20/h3-6,11,13-14,16,18,23H,7-9H2,1-2H3/b10-3+/t11-,13?,14-,16-,18-,20?/m0/s1
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| Chemical Name |
(9R,12R,13Z,16S,17S)-13-ethylidene-6-hydroxy-8-methyl-8,15-diazahexacyclo[14.2.1.01,9.02,7.010,15.012,17]nonadeca-2(7),3,5-trien-18-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~20 mg/mL (~62.03 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2 mg/mL (6.20 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1017 mL | 15.5087 mL | 31.0174 mL | |
| 5 mM | 0.6203 mL | 3.1017 mL | 6.2035 mL | |
| 10 mM | 0.3102 mL | 1.5509 mL | 3.1017 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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