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    MI-3
    MI-3

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V0398
    CAS #: 1271738-59-0Purity ≥98%

    Description: MI-3 (Menin-MLL Inhibitor 3) is a novel and potent inhibitor of menin-MLL interaction with potential antitumor activity. It inhibits menin-MLL interaction with an IC50 of 648 nM. It exhibits excellent antiproliferative activity against various cancer cells.

    References: Nat Chem Biol. 2012 Jan 29;8(3):277-84.

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    Molecular Weight (MW)375.55
    FormulaC18H25N5S2
    CAS No.1271738-59-0
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 19 mg/mL (50.6 mM)          
    Water:<1 mg/mL 
    Ethanol: 19 mg/mL (50.6 mM)     
    Other info

    Chemical Name: 4-[4-(5,5-dimethyl-4H-1,3-thiazol-2-yl)piperazin-1-yl]-6-propan-2-ylthieno[2,3-d]pyrimidine

    InChi Key: FUGQNAUKABUDQI-UHFFFAOYSA-N

    InChi Code: InChI=1S/C18H25N5S2/c1-12(2)14-9-13-15(20-11-21-16(13)24-14)22-5-7-23(8-6-22)17-19-10-18(3,4)25-17/h9,11-12H,5-8,10H2,1-4H3

    SMILES Code: CC(C1=CC2=C(N3CCN(C4=NCC(C)(C)S4)CC3)N=CN=C2S1)C

    SynonymsMI 3; MI-3; MI3


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    In Vitro

    In vitro activity: In HEK293 cells, MI-3 accesses the protein target and effectively inhibits the menin-MLL-AF9 interaction. MI-3 effectively blocks MLL fusion protein-mediated leukemic transformation by downregulating the expression of target genes required for MLL fusion protein oncogenic activity. In human MLL leukemia cell lines harboring different MLL translocations, MI-3 effectively blocks cell proliferation, and induces apoptosis.


    Kinase Assay: FITC-MBM1 at 15 nM and menin at 150 nM in the FP buffer are mixed and incubated for 1h in the dark at room temperature. For point screening, the 0.2 μL of each compound (20 μM final concentration, 1% DMSO) is added to 20 μL of the aliquot of the protein-peptide mixture and incubated on 384-well plates in the dark at room temperature for 1h. In confirmation screening, the serial dilution plates with compounds in DMSO are prepared and used to titrate the menin-FITC-MBM1 complex. Change in fluorescence polarization is monitored at 525 nm after excitations at 495 nm using the PHERAstar microplate reader (BMG) and applied to determine IC50 values with the Origin 7.0 program. 


    Cell Assay: The MLL-AF9 and E2A-HLF transduced murine BMC are plated in 12-well plates at the concentration of 5×103 cells/mL with 1 mL methylcellulose medium M3234 containing 20% IMDM medium, 1% penicillin/streptomycin, IL-3 and 0.25% DMSO or compounds. 6 days later colonies are stained with 100 μL iodonitrotetrazolium chloride at final concentration of 1 mg/mL, incubated at 37°C for 30 min and counted. To replate for the 2nd round, colonies are counted at day 6 without staining and cells were washed out by 1×PBS buffer and resuspended in IMDM medium containing 15% FBS, 1% penicillin/streptomycin and IL-3. 5×103 cells are plated in 12-well plates with 1ml methylcellulose medium M3234 containing 20% IMDM medium, 1% penicillin/streptomycin, IL-3 and 0.25% DMSO or compounds. 6 days later colonies are stained and counted.

    In VivoMLL-AF9 transformed BMC that remained viable after 7 days of treatment with MI-2 and MI-3 showed substantial changes in morphology, indicative of monocytic differentiation, as evidenced by increased cell size, lower nuclear to cytoplasmic ratio and highly vacuolated cytoplasm. Consistent with the change in cell morphology, the expression of CD11b was substantially increased on MLL-AF9 transformed BMC after 7 days of treatment with MI-2 and MI-3. 
    Animal modelNA
    Formulation & DosageNA
    References

    Nat Chem Biol. 2012 Jan 29;8(3):277-84.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

    MI-3

    Effect of MI-2 and MI-3 in human MLL leukemia cells. Nat Chem Biol. 2012 Jan 29; 8(3): 277–284.



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