| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
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| ln Vitro |
The MTAP cytotoxin 6TG (6'-thioguanine) is much less lethal to MTAP+ cells when 5'-methylthioadenosine is present [3].
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|---|---|
| ln Vivo |
In a mouse xenograft model, the fluorescence of four times distinct MTAP human tumor cell lines was observed when 2FA (2'-fluoroadenine) and MTA (5'-methylthioadenosine) were combined (100 mg/kg MTA; 20 mg/kg 2FA) [3].
|
| Cell Assay |
Cell Viability Assay[3]
Cell Types: MTAP+ and MTAP− NIH3T3 cells, isogenic MTAP+ and MTAP− cell lines derived from human HT1080 fibrosarcoma cell line Tested Concentrations: 6TG and 2FA combined with 10 μM MTA Incubation Duration: 48 hrs (hours) Experimental Results: MTAP+ cells The IC50 concentration increased Dramatically in MTAP− cells but did not change Dramatically. |
| References |
[1]. Yaofeng Li, et al. 5'-Methylthioadenosine and Cancer: old molecules, new understanding. J Cancer. 2019;10(4):927-936.
[2]. Tang Y, et al. 5'-Methylthioadenosine attenuates ischemia reperfusion injury after liver transplantation in rats. Inflammation. 2014;37(5):1366-1373. [3]. Li Y, et al. 5'-Methylthioadenosine and Cancer: old molecules, new understanding. J Cancer. 2019;10(4):927-936. [4]. Tang B, et al. Specific Targeting of MTAP-Deleted Tumors with a Combination of 2'-Fluoroadenine and 5'-Methylthioadenosine. Cancer Res. 2018;78(15):4386-4395. |
| Additional Infomation |
5'-S-methyl-5'-thioadenosine is adenosine with the hydroxy group at C-5' substituted with a methylthio (methylsulfanyl) group. It has a role as a human metabolite, an algal metabolite, a Saccharomyces cerevisiae metabolite, an Escherichia coli metabolite and a mouse metabolite. It is functionally related to an adenosine.
5'-Methylthioadenosine is a metabolite found in or produced by Escherichia coli (strain K12, MG1655). 5'-Deoxy-5'-methylthioadenosine has been reported in Drosophila melanogaster, Psychotria punctata, and other organisms with data available. 5'-Methylthioadenosine is a metabolite found in or produced by Saccharomyces cerevisiae. |
| Molecular Formula |
C11H15N5O3S
|
|---|---|
| Molecular Weight |
297.3335
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| Exact Mass |
297.089
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| CAS # |
2457-80-9
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| Related CAS # |
5'-Methylthioadenosine-d3;174838-38-1;5'-Methylthioadenosine-13C6;2421187-73-5
|
| PubChem CID |
439176
|
| Appearance |
Off-white to light yellow solid powder
|
| Density |
1.9±0.1 g/cm3
|
| Boiling Point |
642.7±65.0 °C at 760 mmHg
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| Melting Point |
210-213ºC (dec.)
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| Flash Point |
342.5±34.3 °C
|
| Vapour Pressure |
0.0±2.0 mmHg at 25°C
|
| Index of Refraction |
1.843
|
| LogP |
0.67
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
8
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
20
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| Complexity |
352
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
S(C([H])([H])[H])C([H])([H])[C@]1([H])[C@]([H])([C@]([H])([C@]([H])(N2C([H])=NC3=C(N([H])[H])N=C([H])N=C23)O1)O[H])O[H]
|
| InChi Key |
WUUGFSXJNOTRMR-IOSLPCCCSA-N
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| InChi Code |
InChI=1S/C11H15N5O3S/c1-20-2-5-7(17)8(18)11(19-5)16-4-15-6-9(12)13-3-14-10(6)16/h3-5,7-8,11,17-18H,2H2,1H3,(H2,12,13,14)/t5-,7-,8-,11-/m1/s1
|
| Chemical Name |
(2R,3R,4S,5S)-2-(6-aminopurin-9-yl)-5-(methylsulfanylmethyl)oxolane-3,4-diol
|
| Synonyms |
5Deoxy5(methylthio)adenosine; MTA; Methylthioadenosine
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| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~168.16 mM)
DMF : 2.5 mg/mL (~8.41 mM) H2O : ~1 mg/mL (~3.36 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.41 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.41 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.41 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 1 mg/mL (3.36 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3633 mL | 16.8163 mL | 33.6327 mL | |
| 5 mM | 0.6727 mL | 3.3633 mL | 6.7265 mL | |
| 10 mM | 0.3363 mL | 1.6816 mL | 3.3633 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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