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| 5mg |
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| 10mg |
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| Targets |
Amyloid β (Aβ) plaques, neurofibrillary tangles, cerebrovascular amyloid.
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| ln Vitro |
Methoxy-X04 displays high in vitro binding affinity for amyloid β aggregates with a Ki of 26.8 nM. It binds selectively to fibrillar β-sheet deposits. The compound stains Aβ plaques, tangles, and cerebrovascular amyloid in postmortem brain sections with good specificity. It is commonly utilized for high-resolution fluorescent imaging of individual amyloid plaques in live animals by two-photon microscopy (excitation 750-800 nm).
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| ln Vivo |
Methoxy-X04 is brain penetrant and can cross the blood-brain barrier. It is used to identify amyloid β deposits in the brain. The compound stains Aβ plaques, tangles, and cerebrovascular amyloid in vivo. It is commonly utilized for high-resolution fluorescent imaging of individual amyloid plaques in live animals by two-photon microscopy.
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| Enzyme Assay |
In vitro binding assays measure the affinity of Methoxy-X04 for synthetic Aβ aggregates. Aβ1-42 or Aβ1-40 peptides are aggregated and immobilized on filter membranes or in microtiter plates. Methoxy-X04 at varying concentrations (0.1-1000 nM) is incubated with the aggregated Aβ. Bound fluorescence is measured, and Ki values are calculated from binding curves. Selectivity is assessed against non-fibrillar proteins and other amyloid proteins.
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| Cell Assay |
In vitro cellular assays are performed using brain tissue sections from Alzheimer's disease patients or transgenic mouse models. Sections are incubated with Methoxy-X04 at concentrations ranging from 0.1-10 µM for 10-30 minutes. After washing, fluorescence is visualized by microscopy. Co-staining with thioflavin S or immunohistochemical markers (e.g., anti-Aβ antibodies) confirms specificity. Quantitative analysis of plaque burden is performed using image analysis software.
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| Animal Protocol |
In vivo imaging is performed in transgenic mouse models of Alzheimer's disease (e.g., APP/PS1 mice). Methoxy-X04 is administered intravenously or intraperitoneally. Two-photon microscopy is used to visualize individual amyloid plaques in live animals through cranial windows (excitation 750-800 nm). Plaque burden and dynamics are monitored over time. Brain penetration is confirmed by measuring fluorescence in brain tissue.
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| ADME/Pharmacokinetics |
Methoxy-X04 has a molecular weight of approximately 350 g/mol. CAS number: 863918-78-9. Purity ≥98% by HPLC. Store as a powder at -20°C, protected from light. Soluble in DMSO. The compound is lipophilic and brain penetrant. For in vivo use, formulate in suitable vehicle (e.g., saline with DMSO or cyclodextrin). Excitation/emission: ~380 nm / ~500 nm.
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| Toxicity/Toxicokinetics |
No detailed toxicity data is publicly available for Methoxy-X04. As a research imaging probe, it is not intended for human use and has not undergone formal toxicological evaluation. Standard safety assessments would include cytotoxicity profiling and assessment of non-specific binding. The compound is for research use only. Handle with appropriate safety precautions.
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| References |
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| Additional Infomation |
Methoxy-X04 is a research-grade fluorescent amyloid β probe for imaging amyloid plaques in Alzheimer's disease research. It is a derivative of Congo red and Chrysamine-G. The compound is brain penetrant and can be used for in vivo two-photon microscopy. It stains Aβ plaques, tangles, and cerebrovascular amyloid. Methoxy-X04 is supplied as a powder and should be stored protected from light at -20°C.
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| Molecular Formula |
C23H20O3
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|---|---|
| Molecular Weight |
344.403106689453
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| Exact Mass |
344.141
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| CAS # |
863918-78-9
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| PubChem CID |
16049314
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| Appearance |
Light yellow to light brown solid powder
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| LogP |
5.447
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
26
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| Complexity |
456
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| Defined Atom Stereocenter Count |
0
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| SMILES |
COC1=C(C=CC(=C1)/C=C/C2=CC=C(C=C2)O)/C=C/C3=CC=C(C=C3)O
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| InChi Key |
FGYNZFHVGOFCMD-KHVHPYDTSA-N
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| InChi Code |
InChI=1S/C23H20O3/c1-26-23-16-19(3-2-17-6-12-21(24)13-7-17)5-11-20(23)10-4-18-8-14-22(25)15-9-18/h2-16,24-25H,1H3/b3-2+,10-4+
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| Chemical Name |
4-[(E)-2-[4-[(E)-2-(4-hydroxyphenyl)ethenyl]-3-methoxyphenyl]ethenyl]phenol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~72.59 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (3.63 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 1.25 mg/mL (3.63 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9036 mL | 14.5180 mL | 29.0360 mL | |
| 5 mM | 0.5807 mL | 2.9036 mL | 5.8072 mL | |
| 10 mM | 0.2904 mL | 1.4518 mL | 2.9036 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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