| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
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| ln Vitro |
Meclocycline hydrochloride (10, 20 μM; for 48 hours) inhibits the α-smooth muscle that TGF-β1 induces in primary alveolar epithelial cells. It reacts to TGF-β1-induced non-Smad buffering, such as c-Jun N-telogen, p38, and Akt activation, but it does not inhibit the reactivation of Smad or β-catenin. -Jun N had no influence on lung blast fibroblasts' TGF-β1 response, p38 or Akt activation, or termination of cleavage [1].
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| ln Vivo |
In animal, meclocycline hydrochloride (100 mg/kg/day; intraperitoneally; starting 10 days after intratracheal bleomycin) raises newborn rates at day 17. Typical EMT markers generated by bleomycin, Snail1, Twist1, collagen I, as well as fibrinogen and mRNA, were fragmented by melocycline hydrochloride (day 17) [1].
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| References |
[1]. Ying Xi, et al. Inhibition of epithelial-to-mesenchymal transition and pulmonary fibrosis by methacycline. Am J Respir Cell Mol Biol. 2014 Jan;50(1):51-60.
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| Additional Infomation |
Methacycline Hydrochloride can cause developmental toxicity according to state or federal government labeling requirements.
Methacycline hydrochloride is an organic molecular entity. A broad-spectrum semisynthetic antibiotic related to TETRACYCLINE but excreted more slowly and maintaining effective blood levels for a more extended period. See also: Methacycline Hydrochloride (annotation moved to). |
| Molecular Formula |
C22H22N2O8
|
|---|---|
| Molecular Weight |
442.4187
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| Exact Mass |
478.114
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| CAS # |
3963-95-9
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| Related CAS # |
914-00-1; 41672-87-1 (trolamine); 3963-95-9 (HCl);
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| PubChem CID |
54685047
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| Appearance |
Typically exists as solid at room temperature
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| Boiling Point |
813.8ºC at 760 mmHg
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| LogP |
1.064
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
33
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| Complexity |
998
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| Defined Atom Stereocenter Count |
5
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| SMILES |
O([H])[C@]12C(=C(C(N([H])[H])=O)C([C@]([H])([C@]1([H])[C@]([H])([C@]1([H])C(=C([H])[H])C3C([H])=C([H])C([H])=C(C=3C(=C1C2=O)O[H])O[H])O[H])N(C([H])([H])[H])C([H])([H])[H])=O)O[H]
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| InChi Key |
VXPSARQTYDZXAO-CCHMMTNSSA-N
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| InChi Code |
InChI=1S/C22H22N2O8.ClH/c1-7-8-5-4-6-9(25)11(8)16(26)12-10(7)17(27)14-15(24(2)3)18(28)13(21(23)31)20(30)22(14,32)19(12)29;/h4-6,10,14-15,17,25-27,30,32H,1H2,2-3H3,(H2,23,31);1H/t10-,14-,15+,17+,22+;/m1./s1
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| Chemical Name |
(4S,4aR,5S,5aR,12aR)-4-(dimethylamino)-1,5,10,11,12a-pentahydroxy-6-methylidene-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~104.41 mM)
H2O : ~6.67 mg/mL (~13.93 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.22 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2603 mL | 11.3015 mL | 22.6030 mL | |
| 5 mM | 0.4521 mL | 2.2603 mL | 4.5206 mL | |
| 10 mM | 0.2260 mL | 1.1301 mL | 2.2603 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT02099240 | Terminated | Drug: oral antibiotics Procedure: intravenous antibiotics |
Osteomyelitis | Julio Ramirez | 2014-03-06 | Early Phase 1 |
Products are for research use only; We do not sell to patients
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