| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
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| ln Vitro |
By binding the E3 ligase cereblon (CRBN)-binding ligandpomalidomide to Mcl-1 Ross 1-6 with μM range affinities, PROTAC Mcl1 degrader-1 (Compound C3) induces ubiquitination and phosphosomal degradation of Mcl-1. In HeLa cells, PROTAC Mcl1 degrader-1 (0-10 μM; 0-24 hours) induces indirect depletion of Mcl-1 or Bcl-2 protein in a time-concentrated and dependent manner [1].
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| Cell Assay |
Western Blot Analysis[1]
Cell Types: HeLa cells and H23 cells Tested Concentrations: 0.3, 1.0, 3.0 and 10 μM Incubation Duration: 0 1 (0-2 μM; 24 hrs (hours)) Shows cytotoxicity to H23 cells[1]. -24 hour Experimental Results: Induces time- and concentration-dependent selective depletion of Mcl-1 or Bcl-2 protein in HeLa cells. Induces Mcl-1 depletion and PARP cleavage in H23 cells (0-10 μM; 12 hrs (hours)). |
| References |
| Molecular Formula |
C45H45BRN6O8S
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|---|---|
| Molecular Weight |
909.843209028244
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| Exact Mass |
908.22
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| CAS # |
2163793-38-0
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| PubChem CID |
139035048
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| Appearance |
Light yellow to yellow solid powder
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| LogP |
6
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
19
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| Heavy Atom Count |
61
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| Complexity |
1660
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| Defined Atom Stereocenter Count |
0
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| SMILES |
BrC1C=CC(=CC=1)SC1C=CC2C(N(C(C3=CC=CC=1C=23)=O)CCNC(CCCCC(NCCCCCCNC1=CC=CC2C(N(C(C=21)=O)C1C(NC(CC1)=O)=O)=O)=O)=O)=O
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| InChi Key |
BORXNUWYWZOREQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C45H45BrN6O8S/c46-27-15-17-28(18-16-27)61-35-21-19-32-39-29(35)9-7-10-30(39)42(57)51(43(32)58)26-25-49-37(54)14-4-3-13-36(53)48-24-6-2-1-5-23-47-33-12-8-11-31-40(33)45(60)52(44(31)59)34-20-22-38(55)50-41(34)56/h7-12,15-19,21,34,47H,1-6,13-14,20,22-26H2,(H,48,53)(H,49,54)(H,50,55,56)
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| Chemical Name |
N'-[2-[6-(4-bromophenyl)sulfanyl-1,3-dioxobenzo[de]isoquinolin-2-yl]ethyl]-N-[6-[[2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindol-4-yl]amino]hexyl]hexanediamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~54.95 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.75 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.75 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0991 mL | 5.4955 mL | 10.9909 mL | |
| 5 mM | 0.2198 mL | 1.0991 mL | 2.1982 mL | |
| 10 mM | 0.1099 mL | 0.5495 mL | 1.0991 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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