| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
DNA topoisomerase I.
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|---|---|
| ln Vitro |
Mauritianin is a DNA topoisomerase I inhibitor. It stabilizes the covalent complex between DNA and recombinant human topoisomerase I when used at a concentration of 100 µM. The compound has shown cytoprotective activity.
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| ln Vivo |
Mauritianin inhibits tumor growth and induces cancer cell death by inhibiting the expression of genes involved in carcinogenesis. It antagonized tumor promotion in two-stage carcinogenesis models, likely through activation of immune responses against tumors.
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| Enzyme Assay |
Topoisomerase I inhibition is measured using DNA relaxation assays. Recombinant human topoisomerase I is incubated with supercoiled plasmid DNA and varying concentrations of Mauritianin. The conversion of supercoiled DNA to relaxed DNA is analyzed by agarose gel electrophoresis. Stabilization of the covalent complex is assessed by SDS-K+ precipitation.
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| Cell Assay |
Cancer cell lines are treated with Mauritianin at various concentrations. Cell viability is assessed by MTT or CCK-8 assays. Topoisomerase I inhibition is confirmed by assessing DNA damage markers. Apoptosis is evaluated by Annexin V/PI staining. Gene expression analysis identifies pathways affected by treatment.
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| Animal Protocol |
Mauritianin is evaluated in mouse models of carcinogenesis. Animals are treated with Mauritianin in two-stage carcinogenesis models. Tumor incidence and multiplicity are assessed. Immune responses against tumors are evaluated to determine the mechanism of action.
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| ADME/Pharmacokinetics |
Mauritianin has molecular weight 740.66-740.7 and formula C33H40O19. It is a yellow powder. The compound is soluble in Chloroform, Dichloromethane, Ethyl Acetate, DMSO, and Acetone. Purity is typically ≥98%. Storage is recommended desiccated at -20°C.
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| Toxicity/Toxicokinetics |
Preclinical toxicity data for Mauritianin are limited. As a natural flavonoid glycoside, it is expected to have a favorable safety profile. The compound has shown cytoprotective activity. Standard toxicological assessments would be required for clinical development.
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| References | |
| Additional Infomation |
Mauritianin is a research tool for studying topoisomerase I biology, cancer, and immunomodulation. It is a kaempferol glycoside found in various plants. Its mechanism involves inhibition of topoisomerase I, stabilizing the DNA-enzyme complex and leading to DNA damage and cell death. No clinical trials or approved therapeutic indications exist.
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| Molecular Formula |
C33H40O19
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|---|---|
| Molecular Weight |
740.6593
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| Exact Mass |
740.216
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| CAS # |
109008-28-8
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| Appearance |
Off-white to yellow solid powder
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| Density |
1.74g/cm3
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| Boiling Point |
1051ºC at 760mmHg
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| Flash Point |
331.4ºC
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| Vapour Pressure |
0mmHg at 25°C
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| Index of Refraction |
1.727
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~135.01 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.38 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.38 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (3.38 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3501 mL | 6.7507 mL | 13.5015 mL | |
| 5 mM | 0.2700 mL | 1.3501 mL | 2.7003 mL | |
| 10 mM | 0.1350 mL | 0.6751 mL | 1.3501 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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