| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
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Purity: ≥98%
Manidipine 2HCl (also called CV-4093; CV4093), the dihydrochloride salt form of Manidipine, is a calcium channel blocker/CCB that is used clinically as an antihypertensive agent. It inhibits the voltage-dependent calcium inward currents in smooth muscle cells, which results in systemic vasodilation. Comparing manidipine to other Ca2+ channel antagonists, it is said to exhibit minimal cardiodepression and selectively block T-type Ca2+ channels while having a high selectivity for the vasculature.
| Targets |
CDK7/CycH/MAT1 (IC50 = 0.021 μM); CDK2/Cyc E (IC50 = 0.88 μM); CDK5/p35NCK (IC50 = 3 μM); CDK9/cycT (IC50 = 4.2 μM); CDK1/cycB (IC50 = 8.1 μM); CDK4/Cyc D1 (IC50 = 33 μM); CDK6/cycD1 (IC50 = 47 μM)
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| ln Vitro |
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| ln Vivo |
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| Cell Assay |
The amount of [3H]thymidine incorporated into the DNA of human MCs and the evaluation of cell proliferation are used to quantify the mitogenic effect. To summarize, a 25-mL cell culture bottle is seeded with 1 × 105 quiescent cells, which are maintained in low serum medium containing 0.1% FCS. The next day, the cells are either incubated with low serum medium (abone) or stimulated with PDGF-BB (10 ng/mL) after being preincubated with manidipine (10 nM) for three hours. Every day, the medium is changed, and on days 1, 3, and 5, the cells are counted.
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| Animal Protocol |
Normotensive male Wistar-Kyoto rats and male stroke-prone SHR
1 mg/kg, 3 mg/kg and 10 mg/kg Administered via p.o. |
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| References |
| Molecular Formula |
C35H38N4O6.2HCL
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| Molecular Weight |
683.62
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| Exact Mass |
682.2324904
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| Elemental Analysis |
C, 61.49; H, 5.90; Cl, 10.37; N, 8.20; O, 14.04
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| CAS # |
89226-75-5
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| Related CAS # |
Manidipine;89226-50-6
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| Appearance |
Solid powder
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| SMILES |
CC1=C(C(C(=C(N1)C)C(=O)OCCN2CCN(CC2)C(C3=CC=CC=C3)C4=CC=CC=C4)C5=CC(=CC=C5)[N+](=O)[O-])C(=O)OC.Cl.Cl
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| InChi Key |
JINNGBXKBDUGQT-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C35H38N4O6.2ClH/c1-24-30(34(40)44-3)32(28-15-10-16-29(23-28)39(42)43)31(25(2)36-24)35(41)45-22-21-37-17-19-38(20-18-37)33(26-11-6-4-7-12-26)27-13-8-5-9-14-27;;/h4-16,23,32-33,36H,17-22H2,1-3H3;2*1H
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| Chemical Name |
5-O-[2-(4-benzhydrylpiperazin-1-yl)ethyl] 3-O-methyl 2,6-dimethyl-4-(3-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate;dihydrochloride
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| Synonyms |
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.66 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.66 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. View More
Solubility in Formulation 3: 2% DMSO +30%PEG 300 +ddH2O: 2mg/mL |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4628 mL | 7.3140 mL | 14.6280 mL | |
| 5 mM | 0.2926 mL | 1.4628 mL | 2.9256 mL | |
| 10 mM | 0.1463 mL | 0.7314 mL | 1.4628 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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