| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
|
||
| 250mg |
|
||
| 500mg |
|
||
| 1g |
|
||
| 5g |
|
||
| Other Sizes |
| Targets |
Maltotriose targets the maltose regulon of Escherichia coli, serving as the inducer of this gene expression system. It binds to the maltose-binding protein and is transported into bacterial cells, where it activates the expression of genes involved in maltose metabolism. As a carbohydrate, it is also a substrate for various enzymes including alpha-amylase and maltase. It serves as an energy source for bacteria and other organisms.
|
|---|---|
| ln Vitro |
In vitro, maltotriose serves as an inducer of the maltose regulon in Escherichia coli. It is the smallest maltodextrin capable of inducing this regulon, making it a valuable tool for studying gene expression and carbohydrate metabolism in bacteria. The compound is used as a substrate in enzymatic assays for alpha-amylase and other carbohydrate-active enzymes. It is also used as a standard in carbohydrate analysis.
|
| ln Vivo |
In vivo, maltotriose is a product of starch digestion by alpha-amylase in the human digestive tract. It is further broken down by maltase into glucose for absorption. The compound is an intermediate in carbohydrate metabolism and serves as an energy source. Its presence in the diet reflects the consumption of starch-containing foods. The compound is also found in various food products as a result of starch processing.
|
| Enzyme Assay |
For non-cell enzyme/receptor binding assays, maltotriose is used as a substrate to assess α-amylase and maltase activity. Enzyme activity is measured by monitoring the release of glucose or by chromatographic methods. The compound's interaction with the maltose-binding protein can be studied using binding assays or biophysical techniques such as surface plasmon resonance. Its ability to induce the maltose regulon can be assessed in cell-free transcription assays.
|
| Cell Assay |
For in vitro cell-based assays, maltotriose is used in bacterial culture studies to induce the maltose regulon in E. coli. Bacteria are grown in media containing maltotriose as the sole carbon source, and gene expression is measured by reporter gene assays or RT-PCR. The compound's effects on bacterial growth and metabolism are assessed. It is also used in cell culture studies of carbohydrate metabolism in mammalian cells.
|
| Animal Protocol |
For in vivo animal experiments, maltotriose can be administered orally to study carbohydrate digestion and metabolism. The compound's digestion by intestinal enzymes and absorption can be assessed. Its effects on blood glucose levels can be studied in glucose tolerance tests. The compound can also be used as a dietary component in nutritional studies. Its metabolism and effects on gut microbiota can be investigated in animal models.
|
| ADME/Pharmacokinetics |
Maltotriose has a molecular weight of 504.44 g/mol and molecular formula C₁₈H₃₂O₁₆. It is a trisaccharide composed of three glucose units linked by α-1,4-glycosidic bonds. The compound appears as a white crystalline powder and is highly soluble in water. It should be stored under dry conditions for stability. Purity is typically ≥90-95% for research applications. The compound is stable under recommended storage conditions.
|
| Toxicity/Toxicokinetics |
Maltotriose is a carbohydrate that is generally recognized as safe (GRAS) as a food ingredient. It is a natural product of starch digestion and is consumed in the diet. No significant toxicity has been reported. The compound is for research and food ingredient use. It is not a pharmaceutical drug. Standard safety precautions for handling carbohydrates should be followed.
|
| References | |
| Additional Infomation |
α-Malttriose is a maltotriose trisaccharide with a pyranose ring at the reducing end and an α-configuration at the terminal carbon atom. It is a human metabolite. Amylose is being studied in the clinical trial NCT01027325 (resistant starch insulin sensitivity test). Maltotriose is a metabolite present in or produced by Escherichia coli (K12 strain, MG1655 strain). α-Malttriose has been reported in Drosophila melanogaster, humans, and other organisms with relevant data. See also: Maltotriose (note moved to).
Maltotriose is a trisaccharide composed of three glucose molecules linked by α-1,4-glycosidic bonds. It is produced by the action of alpha-amylase on amylose in starch and is the smallest maltodextrin that induces the maltose regulon in E. coli. The compound is used as a research tool to study carbohydrate metabolism, gene expression, and enzyme activity. It is also used as a food ingredient and carbohydrate standard. No clinical trials have been conducted for this compound as a therapeutic agent. |
| Molecular Formula |
C18H32O16
|
|---|---|
| Molecular Weight |
504.4371
|
| Exact Mass |
504.169
|
| CAS # |
1109-28-0
|
| PubChem CID |
192826
|
| Appearance |
White to off-white solid powder
|
| Density |
1.8±0.1 g/cm3
|
| Boiling Point |
958.9±65.0 °C at 760 mmHg
|
| Melting Point |
132-135 °C
|
| Flash Point |
329.7±27.8 °C
|
| Vapour Pressure |
0.0±0.6 mmHg at 25°C
|
| Index of Refraction |
1.652
|
| LogP |
-4.11
|
| Hydrogen Bond Donor Count |
11
|
| Hydrogen Bond Acceptor Count |
16
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
34
|
| Complexity |
641
|
| Defined Atom Stereocenter Count |
15
|
| SMILES |
C([C@@H]1[C@H]([C@@H]([C@H]([C@H](O1)O[C@@H]2[C@H](O[C@@H]([C@@H]([C@H]2O)O)O[C@@H]3[C@H](O[C@@H]([C@@H]([C@H]3O)O)O)CO)CO)O)O)O)O
|
| InChi Key |
FYGDTMLNYKFZSV-PXXRMHSHSA-N
|
| InChi Code |
InChI=1S/C18H32O16/c19-1-4-7(22)8(23)12(27)17(31-4)34-15-6(3-21)32-18(13(28)10(15)25)33-14-5(2-20)30-16(29)11(26)9(14)24/h4-29H,1-3H2/t4-,5-,6-,7-,8+,9-,10-,11-,12-,13-,14-,15-,16+,17-,18-/m1/s1
|
| Chemical Name |
(2R,3R,4S,5S,6R)-2-[(2R,3S,4R,5R,6R)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2R,3S,4R,5R,6S)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~250 mg/mL (~495.60 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (198.24 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9824 mL | 9.9120 mL | 19.8240 mL | |
| 5 mM | 0.3965 mL | 1.9824 mL | 3.9648 mL | |
| 10 mM | 0.1982 mL | 0.9912 mL | 1.9824 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
