| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
|
||
| 100mg |
|
||
| 250mg | |||
| Other Sizes |
| Targets |
Amylase [1]
|
|---|---|
| ln Vitro |
- Maltotetraose serves as a specific substrate for amylase in vitro. It is hydrolyzed by amylase (from serum or urine) into smaller maltose units, which are further converted in the enzyme-coupled reaction system to generate a measurable signal [1]
- The enzyme-coupled assay using Maltotetraose showed a linear relationship between amylase activity and the measured signal over a wide range (0-1000 U/L for serum amylase, 0-2000 U/L for urine amylase) [1] - The assay with Maltotetraose correlated well with the reference method (iodometric assay) for amylase activity determination, with a correlation coefficient (r) of 0.98 for serum and 0.97 for urine [1] - Maltotetraose is not hydrolyzed by non-amylase glycosidases present in serum or urine, ensuring the specificity of the amylase activity assay [1] |
| Enzyme Assay |
- Amylase activity assay (enzyme-coupled method): The reaction mixture contained Maltotetraose (final concentration 5 mM), auxiliary enzymes (maltase, glucose oxidase, peroxidase), and colorimetric substrate. Serum or urine samples (10 μL) were added to the reaction mixture to initiate the reaction [1]
- The reaction was incubated at 37°C for 15 minutes. Amylase in the sample hydrolyzed Maltotetraose to maltose, which was further hydrolyzed by maltase to glucose. Glucose was oxidized by glucose oxidase to produce hydrogen peroxide, which reacted with the colorimetric substrate in the presence of peroxidase to form a colored product [1] - The absorbance of the colored product was measured at 405 nm using a spectrophotometer. Amylase activity was calculated based on a calibration curve constructed with standard amylase solutions [1] - For specificity verification, the assay was performed with samples spiked with non-amylase glycosidases, and no significant interference was observed [1] |
| References | |
| Additional Infomation |
α-D-Glcp-(1->4)-α-D-Glcp-(1->4)-α-D-Glcp-(1->4)-D-Glcp is a maltotetrasaccharide composed of three α-D-glucanose residues and one D-glucanose residue linked by a (1->4) glycosidic bond. Maltotetrasaccharide is a metabolite found or produced in Escherichia coli (K12 strain, MG1655 strain). Straight-chain tetrasaccharides have been reported in Streptomyces, and relevant data are available. Maltotetrasaccharide is a tetrasaccharide composed of four glucose units linked by an α-1,4 glycosidic bond [1]. As a substrate for amylase assays, its key characteristic is its high specificity for amylases and its suitability for enzyme-coupled reactions, which enables rapid and accurate determination of amylase activity in clinical samples. (Serum and urine) [1] - Maltotetrasaccharide-based detection methods are easy to operate, have short incubation time, and are not affected by other carbohydrates or enzymes, making them suitable for routine clinical laboratory applications [1]
|
| Molecular Formula |
C24H42O21
|
|---|---|
| Molecular Weight |
666.5777
|
| Exact Mass |
666.221
|
| CAS # |
34612-38-9
|
| PubChem CID |
439639
|
| Appearance |
White to off-white solid powder
|
| Density |
1.8±0.1 g/cm3
|
| Boiling Point |
1030.9±65.0 °C at 760 mmHg
|
| Flash Point |
577.3±34.3 °C
|
| Vapour Pressure |
0.0±0.6 mmHg at 25°C
|
| Index of Refraction |
1.687
|
| LogP |
-5.83
|
| Hydrogen Bond Donor Count |
14
|
| Hydrogen Bond Acceptor Count |
21
|
| Rotatable Bond Count |
10
|
| Heavy Atom Count |
45
|
| Complexity |
918
|
| Defined Atom Stereocenter Count |
19
|
| SMILES |
C([C@@H]1[C@H]([C@@H]([C@H]([C@H](O1)O[C@@H]2[C@H](O[C@@H]([C@@H]([C@H]2O)O)O[C@@H]3[C@H](O[C@@H]([C@@H]([C@H]3O)O)O[C@@H]4[C@H](OC([C@@H]([C@H]4O)O)O)CO)CO)CO)O)O)O)O
|
| InChi Key |
LUEWUZLMQUOBSB-AYQJAVFRSA-N
|
| InChi Code |
InChI=1S/C24H42O21/c25-1-5-9(29)10(30)15(35)22(40-5)44-19-7(3-27)42-24(17(37)12(19)32)45-20-8(4-28)41-23(16(36)13(20)33)43-18-6(2-26)39-21(38)14(34)11(18)31/h5-38H,1-4H2/t5-,6-,7-,8-,9-,10+,11-,12-,13-,14-,15-,16-,17-,18-,19-,20-,21?,22-,23-,24-/m1/s1
|
| Chemical Name |
(2R,3R,4S,5S,6R)-2-[(2R,3S,4R,5R,6R)-6-[(2R,3S,4R,5R,6R)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2R,3S,4R,5R)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~125 mg/mL (~187.52 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (150.02 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5002 mL | 7.5010 mL | 15.0020 mL | |
| 5 mM | 0.3000 mL | 1.5002 mL | 3.0004 mL | |
| 10 mM | 0.1500 mL | 0.7501 mL | 1.5002 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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