| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
This compound is a chemical linker rather than a drug with a specific biological target. It serves as a conjugation tool for attaching payloads to antibodies or other biomolecules via thiol and amine chemistry.
|
|---|---|
| ln Vitro |
One ligand is for an E3 ubiquitin ligase, and the other is for the target protein; these two ligands are joined by a linker to form PROTACs. PROTACs target and selectively degrade target proteins by taking advantage of the intracellular ubiquitin-proteasome system.
Mal-PEG4-acid does not possess intrinsic biological activity. Its utility is demonstrated through conjugation reactions: the maleimide group forms stable thioether bonds with thiol-containing biomolecules at pH 6.5-7.5, and the carboxylic acid, upon activation with EDC or DCC, forms amide bonds with primary amines at pH 7.4-8.5. |
| ln Vivo |
This compound is not designed for direct in vivo administration. Its in vivo relevance is demonstrated through the efficacy of ADCs synthesized using this linker. The PEG4 spacer provides good aqueous solubility and reduces aggregation of conjugated proteins.
|
| Enzyme Assay |
Conjugation efficiency is assessed by HPLC or LC-MS. The maleimide-thiol reaction is performed at pH 6.5-7.5 in PBS buffer; the carboxylic acid is activated with EDC/NHS to form an NHS ester, which then reacts with primary amines. Reaction progress is monitored by MALDI-TOF or SDS-PAGE.
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| Cell Assay |
No cell-based assays are performed for the linker itself. Cell-based assays are conducted with the final ADC conjugate. These include cell viability assays on target antigen-expressing cancer cell lines, receptor binding assays, and internalization studies by flow cytometry or confocal microscopy.
|
| Animal Protocol |
In vivo efficacy studies are performed with the final ADC conjugate in mouse xenograft models. Tumor-bearing mice are treated with the conjugate via intravenous administration. Tumor growth inhibition, body weight, and survival are monitored. Pharmacodynamic studies assess target modulation in tumor tissues.
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| ADME/Pharmacokinetics |
Mal-PEG4-acid has molecular weight approximately 331.3 and formula C14H21NO7. It is soluble in DMSO, DMF, and aqueous buffers. The PEG4 spacer provides good hydrophilicity. The compound should be stored desiccated at -20°C. As a linker, it is not intended for standalone pharmacokinetic characterization.
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| Toxicity/Toxicokinetics |
As a chemical linker rather than a therapeutic agent, Mal-PEG4-acid has no intrinsic toxicity. Toxicity is assessed at the level of the final ADC conjugate. The PEG moiety is biocompatible. Standard toxicological assessments are performed with the complete conjugate in preclinical development.
|
| Additional Infomation |
Mal-PEG4-acid is a research-grade, non-cleavable PEG linker used for ADC development. It is employed in the synthesis of antibody-drug conjugates where stable attachment of the payload is required. No clinical trials or approved therapeutic applications exist for this linker as a standalone compound.
|
| Molecular Formula |
C15H23NO8
|
|---|---|
| Molecular Weight |
345.34502530098
|
| Exact Mass |
345.142
|
| CAS # |
518044-41-2
|
| PubChem CID |
21881063
|
| Appearance |
Colorless to light yellow Solid-Liquid Mixture
|
| LogP |
-0.6
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
8
|
| Rotatable Bond Count |
15
|
| Heavy Atom Count |
24
|
| Complexity |
419
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O(CCOCCOCCOCCC(=O)O)CCN1C(C=CC1=O)=O
|
| InChi Key |
BTFZSOVKVDCKQD-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C15H23NO8/c17-13-1-2-14(18)16(13)4-6-22-8-10-24-12-11-23-9-7-21-5-3-15(19)20/h1-2H,3-12H2,(H,19,20)
|
| Chemical Name |
3-[2-[2-[2-[2-(2,5-dioxopyrrol-1-yl)ethoxy]ethoxy]ethoxy]ethoxy]propanoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~289.56 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.24 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.24 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8956 mL | 14.4781 mL | 28.9561 mL | |
| 5 mM | 0.5791 mL | 2.8956 mL | 5.7912 mL | |
| 10 mM | 0.2896 mL | 1.4478 mL | 2.8956 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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