| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
The antimicrobial activity of Mafenide Acetate is conferred via inhibition of nucleotide synthesis. [1]
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| ln Vitro |
An in vitro disk diffusion assay was performed to evaluate the antimicrobial activity of reconstituted 5% Mafenide Acetate solution over time. Mueller-Hinton agar plates were seeded with standard suspensions (0.5 McFarland) of Staphylococcus aureus and Pseudomonas aeruginosa. Filter paper disks (1 cm) were saturated with 25 µL of the 5% Mafenide Acetate solution at various time points (0, 2, 7, 14, 30, and 60 days after reconstitution). The saturated disks were placed on the center of the seeded agar plates and incubated at 37°C for 24 hours. The zone of inhibition around each disk was measured. A zone of inhibition of 2 mm or greater was considered indicative of susceptibility. [1]
The 5% Mafenide Acetate solution demonstrated zones of inhibition greater than 2 mm against both S. aureus and P. aeruginosa at all tested time points: 0, 2, 7, 14, 30, and 60 days after reconstitution. There was no decrease in the zone of inhibition between day 0 and day 60. The results were consistent across three independent trials for each time point. [1] Dry, unsaturated filter paper disks used as a control showed no zone of inhibition against either organism. [1] |
| ADME/Pharmacokinetics |
The study indicated that the peak plasma concentration of Mafenide acetate metabolites occurred 2 to 4 hours after administration and decreased to pre-administration levels within 24 hours, indicating rapid tissue absorption. However, the study did not provide detailed pharmacokinetic parameters (e.g., half-life, bioavailability, distribution, excretion). [1]
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| References | |
| Additional Infomation |
Mafenide acetate is a carboxylic acid. Mafenide acetate is the acetate form of mufenib, a synthetic sulfonamide compound and an analogue of aminobenzoic acid (PABA) with topical anti-infective activity. Mafenide acetate competes with PABA for the bacterial enzyme dihydropteroate synthase, thus preventing PABA incorporation into dihydrofolate and interfering with normal folate metabolism. As a result, the de novo synthesis of pyrimidines, which require folate metabolites, is hindered, thereby affecting DNA synthesis. Mafenide acetate has antibacterial activity against a variety of Gram-negative and Gram-positive bacteria, including Pseudomonas aeruginosa and certain anaerobic strains. It is a sulfonamide drug that inhibits carbonic anhydrase and is used as a topical antibacterial agent, particularly suitable for burn treatment. See also: Mafenide (containing the active ingredient). Mafenide acetate is a topical antibacterial agent for burn wounds, particularly suitable for full-thickness burns and burns with eschar or cartilage. [1]
It is effective against both Gram-positive and Gram-negative bacteria, including Pseudomonas aeruginosa. [1] It is known for its ability to penetrate tissues and eschars. [1] It can be used for the prevention or adjunctive treatment of burn wound infections and has been shown to reduce bacterial load and mortality in burn patients. [1] The standard formulation is a 5% solution prepared from 50 g of sterile powder in 1000 mL of sterile water or saline. [1] The package insert recommends discarding any unused solution within 48 hours of opening the container. This study challenges this recommendation, demonstrating sustained in vitro antimicrobial activity for at least 60 days after reconstitution when stored at 4°C. [1] The storage conditions (4°C) used in this study differ from the product's recommended storage conditions (20–25°C). [1] Noteing the high cost of mafennig acetate solution, this study aimed to explore ways to reduce costs by extending its shelf life (over 48 hours). [1] |
| Molecular Formula |
C7H10N2O2S.C2H4O2
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|---|---|
| Molecular Weight |
246.28346
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| Exact Mass |
246.067
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| CAS # |
13009-99-9
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| Related CAS # |
Mafenide hydrochloride;138-37-4;Mafenide;138-39-6
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| PubChem CID |
25590
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| Appearance |
White to off-white solid powder
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| Boiling Point |
382ºC at 760 mmHg
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| Melting Point |
151-152ºC
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| Flash Point |
184.8ºC
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| Vapour Pressure |
4.86E-06mmHg at 25°C
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| LogP |
2.365
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
16
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| Complexity |
255
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
UILOTUUZKGTYFQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C7H10N2O2S.C2H4O2/c8-5-6-1-3-7(4-2-6)12(9,10)11;1-2(3)4/h1-4H,5,8H2,(H2,9,10,11);1H3,(H,3,4)
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| Chemical Name |
acetic acid;4-(aminomethyl)benzenesulfonamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~406.04 mM)
H2O : ≥ 100 mg/mL (~406.04 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.15 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.15 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (10.15 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 70 mg/mL (284.23 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.0604 mL | 20.3021 mL | 40.6042 mL | |
| 5 mM | 0.8121 mL | 4.0604 mL | 8.1208 mL | |
| 10 mM | 0.4060 mL | 2.0302 mL | 4.0604 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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