| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
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| ln Vitro |
Inducing apoptosis and inhibiting Akt–NF-κB signaling, Aophitolic Acid (0-30 µM; 72 hours; HSC-3, SCC2095, and SCC4 cells) exhibits dose-dependent antiproliferative action in oral cancer cells [1]. In SCC4 cells, Aophitolic Acid (0-25 µM; 3-72 hours) activates autophagy and upregulates the expression of two autophagy regulatory proteins and the autophagosome marker LC3B-II [1]. Aophitolic Acid (0-25 µM; 72 hours; SCC4 cells) decreases the expression of the oncogenic E3 ligase MDM2, while increasing p53 phosphorylation and expression [1]. With IC50 values of 17.6 and 22.7 µM, respectively, alphitolic acid (Aophitolic Acid) (0-25 µM; 72 hours; RAW 264.7 macrophages) possesses anti-inflammatory activity and inhibits the production of NO and TNF-α [2].
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|---|---|
| ln Vivo |
In vivo anti-inflammatory action of aophitolic acid (47-756 µg/ear; ih; adult male CF-1 mice) has been shown [3].
|
| Cell Assay |
Cell Viability Assay[1]
Cell Types: HSC-3, SCC2095 and SCC4 Cell Tested Concentrations: 0, 10, 15, 20, 25 and 30 µM Incubation Duration: 72 hrs (hours) Experimental Results: Inhibited proliferation of SCC4 and SCC2095 cells with IC50 values respectively are 12 and 15 µM. Apoptosis analysis[1] Cell Types: SCC4 Cell Tested Concentrations: 0, 10, 20 and 30 µM Incubation Duration: 72 hrs (hours) Experimental Results: The percentage of apoptotic cells increased from 11.8% to 25.1% in a dose-dependent manner. Western Blot Analysis[1] Cell Types: SCC4 Cell Tested Concentrations: 0, 10, 15 and 20 µM Incubation Duration: 72 hrs (hours) Experimental Results: diminished expression of phosphorylated Akt and its downstream substrates including p70S6K, S6 and IκBα. Downregulates the expression of NF-κB and its downstream target gene product Bcl-2. Western Blot Analysis[1] Cell Types: SCC4 Cell Tested Concentrations: 0, 10, 15, 20 and 25 µM Incubation Duration: 3, 6, 12, 24, 48 and 72 hrs (hours) Experimental Results: Autophagosome marker LC3B-II in dose Add time-related methods. The expression of autophagy-related protei |
| Animal Protocol |
Animal/Disease Models: Adult male CF-1 mice [3] Doses: 47, 94, 330, 378 and 756 µg/ear
Route of Administration: subcutaneous injection Experimental Results: Dose-dependent anti-inflammatory activity with ED50 of 0.11 and 0.20 µM . |
| References |
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| Additional Infomation |
2alpha,3beta-dihydroxy-20(29)-lupen-28-oic acid is a pentacyclic triterpenoid that is betulinic acid carrying an additional alpha-hydroxy group at position 2. It has been isolated from Breynia fruticosa. It has a role as a plant metabolite. It is a pentacyclic triterpenoid and a dihydroxy monocarboxylic acid. It is functionally related to a betulinic acid. It derives from a hydride of a lupane.
Alphitolic acid has been reported in Rosa woodsii, Streptomyces pristinaespiralis, and other organisms with data available. |
| Molecular Formula |
C30H48O4
|
|---|---|
| Molecular Weight |
472.69972
|
| Exact Mass |
472.355
|
| CAS # |
19533-92-7
|
| PubChem CID |
12305768
|
| Appearance |
White to off-white solid powder
|
| Density |
1.1±0.1 g/cm3
|
| Boiling Point |
566.4±30.0 °C at 760 mmHg
|
| Flash Point |
310.4±21.1 °C
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| Vapour Pressure |
0.0±3.5 mmHg at 25°C
|
| Index of Refraction |
1.546
|
| LogP |
7.76
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| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
34
|
| Complexity |
894
|
| Defined Atom Stereocenter Count |
11
|
| SMILES |
CC(=C)[C@@H]1CC[C@]2([C@H]1[C@H]3CC[C@H]4[C@]([C@@]3(CC2)C)(CC[C@@H]5[C@@]4(C[C@H]([C@@H](C5(C)C)O)O)C)C)C(=O)O
|
| InChi Key |
PFCVZKFJHRCLCC-PGOIBATFSA-N
|
| InChi Code |
InChI=1S/C30H48O4/c1-17(2)18-10-13-30(25(33)34)15-14-28(6)19(23(18)30)8-9-22-27(5)16-20(31)24(32)26(3,4)21(27)11-12-29(22,28)7/h18-24,31-32H,1,8-16H2,2-7H3,(H,33,34)/t18-,19+,20+,21-,22+,23+,24-,27-,28+,29+,30-/m0/s1
|
| Chemical Name |
(1R,3aS,5aR,5bR,7aR,9R,10R,11aR,11bR,13aR,13bR)-9,10-dihydroxy-5a,5b,8,8,11a-pentamethyl-1-prop-1-en-2-yl-1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydrocyclopenta[a]chrysene-3a-carboxylic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~105.78 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 5 mg/mL (10.58 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1155 mL | 10.5775 mL | 21.1551 mL | |
| 5 mM | 0.4231 mL | 2.1155 mL | 4.2310 mL | |
| 10 mM | 0.2116 mL | 1.0578 mL | 2.1155 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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