| Size | Price | Stock | Qty |
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| 1g |
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| Other Sizes |
| Targets |
Luminol does not have a specific biological target; it is a chemical reagent used for its chemiluminescent properties. Upon oxidation, it emits light at 425 nm (λmax). This reaction is catalyzed by metal ions, such as iron, which is found in hemoglobin, making it useful for blood detection. It is also used in immunoassays where it is coupled to an enzyme, such as horseradish peroxidase (HRP).
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| ln Vitro |
Chemiluminescence (CL) intensity is significantly influenced by the concentration of luminosol. In addition to luminol concentration, other variables that affect luminol chemiluminescence (LCL) intensity include enzyme and oxidant concentrations, pH levels, and other variables. The highest CL intensity ever measured occurred at 0.3 mM luminol concentration. In the region of 0.01 to 0.3 mM, CL intensity rises linearly with increasing luminol concentration. On the other hand, CL intensity decreases with additional increases in luminol concentration [1].
Luminol is not a therapeutic agent and does not have in vitro activity in the traditional pharmacological sense. Its utility lies in its chemiluminescent properties. It is used as a chemiluminescent reagent in various assays, where its oxidation produces a measurable light signal that can be detected and quantified. |
| ln Vivo |
Luminol is not used as a therapeutic agent and does not have in vivo activity in the pharmacological sense. It is a research and diagnostic reagent used for various applications, including the detection of blood stains, metal cations, and as a probe in biological assays.
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| Enzyme Assay |
Non-cellular assays for luminol are based on its chemiluminescent reaction. The compound is oxidized in the presence of an oxidant, such as hydrogen peroxide, and a catalyst, such as horseradish peroxidase (HRP) or a metal ion. The light emission is measured using a luminometer. This reaction can be used to detect the presence of the catalyst or to quantify the activity of enzymes like HRP in a cell-free system.
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| Cell Assay |
In vitro cellular assays with luminol are not standard, as it is a chemiluminescent reagent. However, it can be used as a probe to detect reactive oxygen species (ROS) produced by cells, such as neutrophils. When cells are stimulated, they produce ROS, which can oxidize luminol, resulting in a chemiluminescent signal that can be measured. This assay is used to assess the respiratory burst in phagocytic cells.
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| Animal Protocol |
In vivo animal experiments are not applicable for luminol, as it is a research reagent, not a therapeutic agent. It is not administered to animals for pharmacological studies. Its use is limited to ex vivo applications, such as detecting blood stains in forensic investigations or in various diagnostic assays.
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| ADME/Pharmacokinetics |
Luminol has a molecular weight of 177.16 and a molecular formula of C₈H₇N₃O₂. It is a solid powder with a purity of ≥97%. The compound is typically stored at room temperature in a dry, dark place. It is stable under recommended storage conditions. It has pKa values of 6.74 and 15.1. Its CAS number is 521-31-3.
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| Toxicity/Toxicokinetics |
Luminol is a research compound for laboratory use only and is not intended for human therapeutic or diagnostic use. It may cause skin, eye, and respiratory irritation. Standard laboratory safety precautions should be followed when handling the compound. It is combustible and incompatible with strong oxidizing agents.
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| References | |
| Additional Infomation |
O-aminophthalyl hydrazide is a yellow crystal or light beige powder. (NTP, 1992)
5-Amino-2,3-dihydro-1,4-phthalic acid hydrazide dione. It is a luminescent substance upon oxidation. Used in chemical determinations. Luminol (3-Aminophthalhydrazide; CAS 521-31-3) is a chemical compound known for its chemiluminescent properties. Upon oxidation, it emits a blue glow at 425 nm. It is widely used in forensics for blood detection, in chemiluminescent immunoassays, and as a biological probe. It is available from various commercial suppliers for research applications. |
| Molecular Formula |
C8H7N3O2
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|---|---|
| Molecular Weight |
177.163
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| Exact Mass |
177.053
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| CAS # |
521-31-3
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| Related CAS # |
Luminol sodium salt;20666-12-0
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| PubChem CID |
10638
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
621.9ºC at 760 mmHg
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| Melting Point |
>300 °C(lit.)
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| Flash Point |
329.9ºC
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| Index of Refraction |
1.649
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| LogP |
-0.71
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
13
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| Complexity |
254
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
HWYHZTIRURJOHG-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13)
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| Chemical Name |
5-amino-2,3-dihydrophthalazine-1,4-dione
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| Synonyms |
NSC-5064; NSC 5064; Luminol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 30 mg/mL (~169.34 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (11.74 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.08 mg/mL (11.74 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (11.74 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.6446 mL | 28.2231 mL | 56.4462 mL | |
| 5 mM | 1.1289 mL | 5.6446 mL | 11.2892 mL | |
| 10 mM | 0.5645 mL | 2.8223 mL | 5.6446 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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