| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
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Purity: ≥80%
| Targets |
Fluorescent dye/probe
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|---|---|
| ln Vitro |
Making the working solution of Lucifer Yellow CH (dilithium salt).
1. Preparation of stock solution To make a 1 mg/mL stock solution of Lucifer Yellow CH (dilithium salt), dissolve 1 mg of Lucifer Yellow CH (dilithium salt) in 1 mL of DDH2O. Note: After aliquots, the Lucifer Yellow CH (dilithium salt) storage solution should be kept in the dark at -20°C or -80°C. 2. Utilizing the pre-heated serum-free media or PBS, dilute the stock solution into 0.5–1% Lucifer Yellow CH (dilithium salt) working solution. Note: Before using, please make sure that the concentration of the Lucifer Yellow CH (dilithium salt) working solution is appropriate for the current circumstances. Staining of cells 1. Preparation of cells Suspension cells: Centrifuge cells to collect them, then add PBS and wash twice for five minutes each time. Adherent cells: Add islet digested cells after discarding the culture medium. Centrifuge, remove supernatant, add PBS, and wash twice for five minutes each time. 2. Add 1 mL of the working solution for 5–30 minutes (Lucifer Yellow CH, dilithium salt). 3. Discard the supernatant after centrifuging at 400 g for three to four minutes at 4°C. 4. To wash the cells twice, five times apiece, add PBS. 5. Re-suspend the cells in 1 milliliter of PBS or serum-free water, and observe using a flow cytometer or fluorescence microscope. Note: 1. After aliquoting, the Lucifer Yellow CH (dilithium salt) storage solution should be kept out of the light at -20°C or -80°C. Frequent freezing and thawing should be avoided. It can be kept for one month at -20°C and six months at -80°C. 2. You may adjust the Lucifer Yellow CH (dilithium salt) working solution concentration according to your specific needs. 3. This product should not be used for clinical diagnosis or treatment, nor should it be included into food or medication. It is intended only for research use. 4. Please wear disposable gloves and a lab coat for your health and safety. |
| ln Vivo |
Contrasting observations exist which indicate that in plants the fluorescent dye lucifer yellow CH (LYCH) either can be used as a tracer for endocytosis or as a substrate for an anion transporter located at the vacuolar membrane. In addition, LYCH as a disulphonated substance may represent an analogue of sulphonated or sulfated natural compounds like some flavonoids. We performed uptake experiments with LYCH into isolated rye vacuoles and observed saturable (Km = 0.3-0.6 mM) vacuolar transport and accumulation of the dye against the concentration gradient only when MgATP was present. GTP and, to a low extent, UTP could substitute for ATP, while the non-hydrolysable ATP analogue AMP-PNP did not drive LYCH uptake. Vanadate and probenecid, the latter substance is known to inhibit organic anion transport at the liver canalicular membrane, both strongly decreased the vacuolar uptake of LYCH, while bafilomycin A1, a specific inhibitor of the vacuolar H+-ATPase, had no effect. Together with the fact that abolishment of the delta pH via CCCP had only a weak influence on LYCH accumulation, our results indicate that this compound is taken up into rye vacuoles by a directly energized process. Uptake of LYCH was strongly inhibited by other sulfated compounds including sulfobromophthalein and the flavones apigenin 7,4'-disulfate and luteolin 7,4'-disulfate arguing for the presence of a vacuolar transporter for structurally different sulphonated or sulfated compounds. Glucuronates like the rye-specific flavone luteolin 7-O-diglucuronide also strongly decreased uptake of the dye, whereas only a weak effect was observed in the presence of glutathione and a glutathione conjugate, suggesting that LYCH uptake is not mediated via the vacuolar glutathione conjugate pump [2].
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| References |
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| Additional Infomation |
Lucifer yellow carbohydrazide dye is an organic dilithium salt having 6-amino-2-[(hydrazinocarbonyl)amino]-1,3-dioxo-2,3-dihydro-1H-benzo[de]isoquinoline-5,8-disulfonate as the counterion. It has a role as a fluorochrome. It contains a lucifer yellow carbohydrazide dye(2-).
|
| Molecular Formula |
C13H9LI2N5O9S2
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|---|---|
| Molecular Weight |
457.25100
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| Exact Mass |
457.016
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| Elemental Analysis |
C, 34.15; H, 1.98; Li, 3.04; N, 15.32; O, 31.49; S, 14.02
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| CAS # |
67769-47-5
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| Related CAS # |
Lucifer Yellow CH dipotassium salt;71206-95-6
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| PubChem CID |
93367
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| Appearance |
Yellow to orange solid
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| LogP |
1.376
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
31
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| Complexity |
914
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=C(C=C2C3=C1C(=C(C=C3C(=O)N(C2=O)NC(=NN)[O-])S(=O)(=O)O)N)S(=O)(=O)[O-].[Li+].[Li+]
|
| InChi Key |
RPKCZJYDUKVMGF-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C13H11N5O9S2.2Li/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2
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| Chemical Name |
1H-Benz(de)isoquinoline-5,8-disulfonic acid, 6-amino-2-((hydrazinocarbonyl)amino)-2,3-dihydro-1,3-dioxo-, dilithium salt
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| HS Tariff Code |
2934.99.03.00
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~25 mg/mL (~54.67 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 10 mg/mL (21.87 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1870 mL | 10.9349 mL | 21.8699 mL | |
| 5 mM | 0.4374 mL | 2.1870 mL | 4.3740 mL | |
| 10 mM | 0.2187 mL | 1.0935 mL | 2.1870 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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