| Size | Price | |
|---|---|---|
| Other Sizes |
Purity: Specification: Dealkaline
| Targets |
Lignin does not have a specific pharmacological target. Its biological role is structural, providing rigidity and water impermeability to plant cell walls. In research, it is studied for its antioxidant properties and its potential as a natural polymer for various biomedical and industrial applications.
|
|---|---|
| ln Vitro |
Looking at it from a structural perspective, lignin is a multifunctional natural phenolic polymer that is created in the cell walls of all vascular plants by coupling three hydroxypropyl alcohols together using free radicals: coumarin, coniferyl, and glucosinolates. Natural antioxidants have the potential to do so[1].
In vitro, Lignin demonstrates strong antioxidant activity. It is used in cell culture studies to assess its cytotoxic or anti-proliferative effects on tumor cell lines. Its ability to scavenge free radicals makes it a compound of interest for research into oxidative stress and its related pathologies. |
| ln Vivo |
In vivo, Lignin is not used as a therapeutic agent. However, its antioxidant properties suggest potential health benefits, such as reducing oxidative stress. Studies have investigated the acute toxicity of lignin-based polymers, indicating that they are generally safe. Its biodegradability and biocompatibility make it a promising material for drug delivery.
|
| Enzyme Assay |
The in vitro antioxidant assay for Lignin involves measuring its ability to scavenge free radicals, such as DPPH or ABTS. The compound is incubated with the radical, and the decrease in absorbance is measured. For cytotoxicity, cancer cell lines are treated with Lignin, and cell viability is assessed using an MTT assay.
|
| Cell Assay |
In vitro cell culture studies for Lignin utilize various cancer cell lines to assess its potential anti-proliferative effects. Cells are treated with the compound, and cell viability is measured using the MTT or SRB assay. The production of reactive oxygen species (ROS) is also measured to evaluate its antioxidant effects.
|
| Animal Protocol |
In vivo animal experiments for Lignin are limited. However, acute toxicity studies have been performed in animal models to assess the safety of lignin-based materials. In these studies, the compound is administered orally or intraperitoneally, and parameters such as body weight, organ weight, and histopathology are assessed.
|
| ADME/Pharmacokinetics |
Lignin is a high molecular weight polymer with a complex and variable structure. It is a brown or black powder that is insoluble in water and most organic solvents. It is typically stored at room temperature in a dry place. Its stability is maintained under recommended storage conditions.
|
| Toxicity/Toxicokinetics |
Toxicological data for Lignin indicate that it is generally considered non-toxic and biocompatible. Acute toxicity studies have shown that it is safe at moderate doses. However, as a research compound, it is not intended for human therapeutic use, and standard safety precautions should be followed.
|
| References | |
| Additional Infomation |
Lignin is a research compound with no clinical approval. It is a valuable material for studying plant cell wall structure and for developing sustainable biomaterials. Its strong antioxidant activity makes it a compound of interest for research into oxidative stress and its related diseases. It is also used as a precursor for the production of various chemicals and materials.
|
| Molecular Formula |
C81H92O28
|
|---|---|
| Molecular Weight |
1513.58038
|
| Exact Mass |
508.993
|
| CAS # |
9005-53-2
|
| PubChem CID |
175586
|
| Appearance |
Brown to black solid powder
|
| LogP |
-0.8
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
10
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
33
|
| Complexity |
863
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
NBNRHKNCHFFYTL-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C10H11N5O2/c1-7-10(17)9(8(4-16)2-11-7)3-14-15-5-12-13-6-15/h2-3,5-6,16-17H,4H2,1H3
|
| Chemical Name |
5-(hydroxymethyl)-2-methyl-4-(1,2,4-triazol-4-yliminomethyl)pyridin-3-ol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~12.5 mg/mL
H2O : < 0.1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.71 mg/mL (Infinity mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 7.1 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.6607 mL | 3.3034 mL | 6.6069 mL | |
| 5 mM | 0.1321 mL | 0.6607 mL | 1.3214 mL | |
| 10 mM | 0.0661 mL | 0.3303 mL | 0.6607 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
COA