| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Licoflavone B targets enzymes essential for parasite survival. It inhibits Schistosoma mansoni ATPase and ADPase activities. It also exhibits significant antiviral activity against the influenza virus. Its mechanism of action likely involves the disruption of critical metabolic pathways in parasites and viruses, although the exact binding sites and interactions are still under investigation.
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| ln Vitro |
In vitro, Licoflavone B demonstrates potent inhibitory activity against S. mansoni ATPase with an IC50 of 23.78 μM and ADPase with an IC50 of 31.50 μM. It also shows significant antiviral activity against the influenza A/Puerto Rico/8/34 (H1N1) virus with an IC50 of 6.7 μM. These activities suggest a potential for development as an anti-schistosomiasis and anti-influenza agent.
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| ln Vivo |
In vivo, Licoflavone B has not been extensively studied in animal models. Its anti-schistosomiasis activity, demonstrated by the inhibition of ATPase and ADPase, suggests potential in vivo efficacy against S. mansoni infection. However, specific standalone in vivo data for this compound are limited. Its antiviral activity also suggests potential for in vivo efficacy against influenza virus infection.
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| Enzyme Assay |
The in vitro enzyme assay for Licoflavone B involves measuring its ability to inhibit ATPase and ADPase activities from S. mansoni. The enzyme is incubated with the substrate (ATP or ADP) in the presence of varying concentrations of the compound. The amount of phosphate released is measured using a colorimetric method, and the IC50 is calculated from the inhibition curve. Antiviral activity is assessed by plaque reduction assays using influenza virus-infected cells.
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| Cell Assay |
In vitro cell culture studies for Licoflavone B utilize influenza virus-infected cell lines, such as MDCK cells, to assess its antiviral activity. Cells are infected with the virus and treated with varying concentrations of the compound. Viral replication is measured by plaque reduction or by quantifying viral RNA using RT-qPCR. The compound's cytotoxicity is assessed in parallel to determine its selectivity index.
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| Animal Protocol |
In vivo animal experiments for Licoflavone B are not extensively documented. However, its antiviral activity could be evaluated in mouse models of influenza infection. The compound would be administered orally or intraperitoneally, and parameters such as survival rate, viral load in the lungs, and weight loss would be measured. Its anti-schistosomiasis activity could be tested in mouse models of S. mansoni infection.
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| ADME/Pharmacokinetics |
Licoflavone B is a flavonoid with a molecular weight of 406.47 g/mol. It is a solid compound that is soluble in organic solvents like DMSO. It is typically stored as a powder at -20°C for long-term stability. As a natural product, it is often isolated from plant sources and is used as an analytical standard. Its stability is maintained under recommended storage conditions.
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| Toxicity/Toxicokinetics |
Toxicological data for Licoflavone B are limited, as it is a research compound. As a natural flavonoid, it is generally considered to have low toxicity. However, as a research compound, it is not intended for human therapeutic use, and comprehensive toxicity studies are not available. Standard safety precautions should be followed.
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| References | |
| Additional Infomation |
Glycyrrhizin B is a flavonoid compound. It has been reported to exist in white lupin (Lupinus albus), glabra licorice (Glycyrrhiza glabra), and inflata licorice (Glycyrrhiza inflata), and relevant data are available. See also: Glycyrrhiza inflata root (partial).
Licoflavone B is a research compound with no clinical approval. It is a valuable tool for studying the pharmacology of flavonoids and their potential applications in infectious diseases. Its ATPase and ADPase inhibitory activities make it a compound of interest for research into schistosomiasis. Its antiviral activity against influenza virus also makes it a candidate for further study as an antiviral agent. |
| Molecular Formula |
C25H26O4
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|---|---|
| Molecular Weight |
390.4715
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| Exact Mass |
390.183
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| CAS # |
91433-17-9
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| PubChem CID |
11349817
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| Appearance |
White to yellow solid powder
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| Density |
1.191±0.06 g/cm3(Predicted)
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| Boiling Point |
593.9±50.0 °C(Predicted)
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| LogP |
6.3
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
29
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| Complexity |
681
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
GLDVIKFETPAZNV-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C25H26O4/c1-15(2)5-7-17-11-19(9-10-21(17)26)24-14-23(28)20-12-18(8-6-16(3)4)22(27)13-25(20)29-24/h5-6,9-14,26-27H,7-8H2,1-4H3
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| Chemical Name |
7-hydroxy-2-[4-hydroxy-3-(3-methylbut-2-enyl)phenyl]-6-(3-methylbut-2-enyl)chromen-4-one
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| Synonyms |
Licoflavone B; Prenyllicoflavone A; VMN9VGP9XJ;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~256.10 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.40 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5610 mL | 12.8051 mL | 25.6102 mL | |
| 5 mM | 0.5122 mL | 2.5610 mL | 5.1220 mL | |
| 10 mM | 0.2561 mL | 1.2805 mL | 2.5610 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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