| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
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| 10mg | |||
| Other Sizes |
| Targets |
Licochalcone E targets multiple signaling pathways including NF-κB, AP-1, AKT, MAPK, p38 MAPK, and LXRβ; it inhibits the transcriptional activities of NF-κB and AP-1 by inhibiting AKT and MAPK activation, and acts as a potential LXRβ agonist.
|
|---|---|
| ln Vitro |
In vitro studies demonstrate that Licochalcone E inhibits NF-κB and AP-1 transcriptional activities through inhibition of AKT and MAPK signaling pathways, and functions as a p38 MAPK inhibitor and a potential LXRβ agonist, with anti-inflammatory and hepatoprotective effects.
|
| ln Vivo |
In vivo, Licochalcone E has been studied for the treatment of hepatotoxicity, exhibiting protective effects primarily through a PPARγ/NF-κB-mediated pathway, though detailed in vivo efficacy data are more limited compared to in vitro findings.
|
| Enzyme Assay |
The in vitro transcription factor activity assay involves treating cells with Licochalcone E and measuring NF-κB and AP-1 DNA binding activity via EMSA or reporter gene assays (e.g., luciferase reporter constructs), with inhibition calculated relative to untreated or stimulated controls.
|
| Cell Assay |
In vitro cell culture studies utilize various cell lines (e.g., macrophages, hepatocytes) treated with Licochalcone E, assessing inflammatory cytokine production by ELISA, NF-κB and MAPK pathway activation by Western blotting, cell viability by MTT, and hepatoprotective effects against toxin-induced damage.
|
| Animal Protocol |
In vivo animal experiments involve administering Licochalcone E to rodent models of hepatotoxicity (e.g., CCl4-induced liver injury) or inflammation, with assessment of liver enzyme levels (ALT, AST) in serum, histopathological analysis of liver tissues, and measurement of inflammatory markers by ELISA or qPCR.
|
| ADME/Pharmacokinetics |
Licochalcone E has a molecular weight of 338.40 g/mol and is typically stored as a solid powder at 4°C protected from light; it is soluble in DMSO, with solutions stable at -80°C for up to 6 months and at -20°C for up to 1 month when protected from light.
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| Toxicity/Toxicokinetics |
Toxicological data for Licochalcone E are limited; as a natural flavonoid from licorice, it is generally considered to have low toxicity, though comprehensive safety profiles in animals have not been extensively reported; standard precautions for handling research compounds should be observed.
|
| References | |
| Additional Infomation |
Reports have indicated that licorice contains Licochalcone E, and relevant data is available for reference.
Licochalcone E is a research compound used for studying the anti-inflammatory, hepatoprotective, and anticancer mechanisms of chalcones and licorice-derived flavonoids; it has not been approved for clinical use and serves as a valuable tool for investigating NF-κB, MAPK, and LXR signaling pathways. |
| Molecular Formula |
C21H22O4
|
|---|---|
| Molecular Weight |
338.396986484528
|
| Exact Mass |
338.151
|
| CAS # |
864232-34-8
|
| PubChem CID |
46209991
|
| Appearance |
Yellow to orange solid powder
|
| LogP |
5
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
25
|
| Complexity |
490
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
C(/C1C=C([C@@H](C)C(=C)C)C(O)=CC=1OC)=C\C(C1C=CC(O)=CC=1)=O
|
| InChi Key |
SWPKMTGYQGHLJS-RNVIBTMRSA-N
|
| InChi Code |
InChI=1S/C21H22O4/c1-13(2)14(3)18-11-16(21(25-4)12-20(18)24)7-10-19(23)15-5-8-17(22)9-6-15/h5-12,14,22,24H,1H2,2-4H3/b10-7+/t14-/m0/s1
|
| Chemical Name |
(E)-3-[4-hydroxy-2-methoxy-5-[(2S)-3-methylbut-3-en-2-yl]phenyl]-1-(4-hydroxyphenyl)prop-2-en-1-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~147.75 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.39 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9551 mL | 14.7754 mL | 29.5508 mL | |
| 5 mM | 0.5910 mL | 2.9551 mL | 5.9102 mL | |
| 10 mM | 0.2955 mL | 1.4775 mL | 2.9551 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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