| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Leucyl-phenylalanine targets peptide transporters such as PEPT1 and PEPT2, which are responsible for the intestinal absorption of di- and tripeptides. It is also a substrate for various peptidases, including aminopeptidases and dipeptidases, which hydrolyze the peptide bond to release the constituent amino acids. It may also interact with opioid receptors in some contexts.
|
|---|---|
| ln Vitro |
In vitro, leucyl-phenylalanine is used to study peptide transport across cell membranes using Caco-2 cells or other intestinal epithelial cell models. It can be used as a substrate to measure the activity of peptidases such as aminopeptidase N and dipeptidyl peptidase IV. It may be used to study the effects of peptides on cell signaling and metabolism.
|
| ln Vivo |
In vivo, leucyl-phenylalanine is absorbed from the diet and transported into cells via peptide transporters. It is hydrolyzed by peptidases to release leucine and phenylalanine, which are then used for protein synthesis and other metabolic processes. It plays a role in nutrition and protein metabolism. It may have effects on appetite, satiety, and metabolic regulation.
|
| Enzyme Assay |
For non-cellular enzyme assays, leucyl-phenylalanine is used as a substrate to measure peptidase activity. The enzyme is incubated with the dipeptide, and the release of free amino acids (leucine and phenylalanine) is measured using chromatographic, spectrophotometric, or fluorometric methods. The rate of hydrolysis is determined to assess enzyme activity.
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| Cell Assay |
For in vitro cell-based assays, cells expressing peptide transporters (such as Caco-2 cells) are cultured and treated with leucyl-phenylalanine. Peptide uptake is measured by quantifying intracellular or extracellular peptide and amino acid levels using HPLC or LC-MS. The effects on cell signaling, gene expression, and metabolism can also be assessed.
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| Animal Protocol |
For in vivo studies, leucyl-phenylalanine can be administered orally or intravenously to animal models. Plasma and tissue levels of the peptide and its constituent amino acids are measured. The effects on protein metabolism, nitrogen balance, and nutritional status can be assessed. It can also be used in tracer studies to track peptide absorption and metabolism.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties include solubility in water and DMSO. It is a dipeptide and is rapidly absorbed and metabolized. It is hydrolyzed by peptidases in the intestine, blood, and tissues. The constituent amino acids are used for protein synthesis and other metabolic pathways. Storage is typically at -20°C. It is a solid and should be handled with standard laboratory precautions.
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| Toxicity/Toxicokinetics |
Toxicological data for leucyl-phenylalanine is limited. As a naturally occurring dipeptide, it is generally considered safe and is a normal component of dietary protein. No significant toxicity has been reported. It is not for human use in research settings without appropriate approval.
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| Additional Infomation |
Leucine-phenylalanine (Leu-Phe) is a dipeptide composed of L-leucine and L-phenylalanine residues. It is a metabolite that is functionally related to L-leucine and L-phenylalanine.
Leucyl-phenylalanine is a dipeptide composed of leucine and phenylalanine. It is used as a model substrate for studying peptide transport and metabolism. It is a substrate for peptide transporters and peptidases. It plays a role in nutrition and protein metabolism. It may have applications in studying appetite regulation and metabolic disorders. |
| Molecular Formula |
C15H22N2O3
|
|---|---|
| Molecular Weight |
278.34678
|
| Exact Mass |
278.163
|
| CAS # |
3063-05-6
|
| PubChem CID |
6992310
|
| Appearance |
White to off-white solid powder
|
| Density |
1.144g/cm3
|
| Boiling Point |
501.2ºC at 760mmHg
|
| Flash Point |
256.9ºC
|
| Index of Refraction |
1.544
|
| LogP |
2.263
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
20
|
| Complexity |
325
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
CC(C)C[C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)N
|
| InChi Key |
KFKWRHQBZQICHA-STQMWFEESA-N
|
| InChi Code |
InChI=1S/C15H22N2O3/c1-10(2)8-12(16)14(18)17-13(15(19)20)9-11-6-4-3-5-7-11/h3-7,10,12-13H,8-9,16H2,1-2H3,(H,17,18)(H,19,20)/t12-,13-/m0/s1
|
| Chemical Name |
(2S)-2-[[(2S)-2-amino-4-methylpentanoyl]amino]-3-phenylpropanoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~4 mg/mL (~14.37 mM)
Ethanol : ~1.11 mg/mL (~3.99 mM) DMSO :< 1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2 mg/mL (7.19 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5926 mL | 17.9630 mL | 35.9260 mL | |
| 5 mM | 0.7185 mL | 3.5926 mL | 7.1852 mL | |
| 10 mM | 0.3593 mL | 1.7963 mL | 3.5926 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Link: https://clinicaltrials.gov/ct2/show/NCT05233709
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