| Size | Price | Stock | Qty |
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| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
Leucyl-alanine targets peptide transporters PEPT1 and PEPT2, serving as a model substrate for studying peptide transport mechanisms. These transporters are proton-coupled oligopeptide transporters that mediate the absorption of di- and tripeptides in the intestine and kidney. By using leucyl-alanine as a substrate, researchers can study the kinetics and regulation of peptide transport. The compound also inhibits ubiquitin-mediated protein degradation, suggesting additional effects on the ubiquitin-proteasome pathway.
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| ln Vitro |
In vitro, leucyl-alanine is used as a model substrate for peptide transport studies in cells expressing PEPT1 or PEPT2. Cells are incubated with the dipeptide, and its uptake is measured to assess transporter activity. The compound also inhibits ubiquitin-mediated protein degradation. Detailed IC₅₀ values for transport or degradation inhibition are not extensively reported in the available literature. Its primary use is as a research tool.
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| ln Vivo |
Detailed in vivo activity data for leucyl-alanine are not extensively reported in the available literature. As a dipeptide, it is absorbed through peptide transporters in the intestine and may be hydrolyzed to its constituent amino acids. Its role as a metabolite and its ability to inhibit ubiquitin-mediated protein degradation suggest potential for in vivo effects on protein turnover. However, specific in vivo efficacy data are not provided in the available sources.
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| Enzyme Assay |
Non-cell-based assays for leucyl-alanine are not typical, as it is primarily used as a substrate in cell-based transport studies. However, its purity and identity can be assessed using analytical techniques such as HPLC, LC-MS, and amino acid analysis. As a reference standard, it is used in method development for dipeptide quantification.
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| Cell Assay |
Cellular assays for leucyl-alanine are performed using cells expressing PEPT1 or PEPT2 transporters. Cells are incubated with the dipeptide at various concentrations and times, and intracellular or extracellular peptide levels are measured using HPLC or LC-MS. Transport kinetics (Km, Vmax) are determined. Inhibition studies with other peptides or transporter inhibitors can be performed to characterize the transport mechanism. The compound's effects on ubiquitin-mediated degradation can be assessed by measuring ubiquitinated protein levels.
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| Animal Protocol |
In vivo animal models for leucyl-alanine are not extensively detailed in the available literature. However, as a dipeptide substrate, it could be used in intestinal perfusion studies to assess peptide absorption in animal models. The compound would be administered via intestinal perfusion or oral gavage, and its appearance in the blood or tissues would be measured to assess absorption and metabolism.
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| ADME/Pharmacokinetics |
Leucyl-alanine has a molecular weight of 202.25 g/mol and a molecular formula of C₉H₁₈N₂O₃. CAS number is 7298-84-2. The compound is soluble in DMSO. Purity is >98%. Storage conditions: powder at -20°C for 3 years; in solvent at -20°C for 6 months. The compound is supplied for research use only.
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| Toxicity/Toxicokinetics |
Leucyl-alanine is a dipeptide with generally low toxicity. Detailed toxicological data are not extensively reported, as the compound is not intended for therapeutic use. It is composed of two amino acids that are naturally occurring and essential for human health. Standard laboratory safety precautions should be followed when handling the compound.
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| References | |
| Additional Infomation |
Leucyl-L-alanine (Leu-Ala) is a dipeptide composed of L-leucine and L-alanine linked by a peptide bond. It is a metabolite functionally related to both L-leucine and L-alanine. L-Leucyl-L-alanine has been reported in Paraburkholderia, Vitis vinifera, and Trypanosoma brucei, and relevant data are available.
Leucyl-alanine is also known as L-Leucyl-L-alanine and Leu-Ala. It is a simple dipeptide composed of L-leucine and L-alanine. The compound is a naturally occurring dipeptide found in various food sources. It is primarily used as a model substrate for peptide transport studies involving PEPT1 and PEPT2. It also inhibits ubiquitin-mediated protein degradation. Leucyl-alanine is for research use only. |
| Molecular Formula |
C9H18N2O3
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|---|---|
| Molecular Weight |
202.25
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| Exact Mass |
202.132
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| CAS # |
7298-84-2
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| PubChem CID |
81721
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| Appearance |
White to off-white solid powder
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| Density |
1.108±0.06 g/cm3
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| Melting Point |
255-256 ºC
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| Index of Refraction |
19 ° (C=5, MeOH)
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| LogP |
1.04
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
14
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| Complexity |
216
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| Defined Atom Stereocenter Count |
2
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| SMILES |
C[C@@H](C(=O)O)NC(=O)[C@H](CC(C)C)N
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| InChi Key |
HSQGMTRYSIHDAC-BQBZGAKWSA-N
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| InChi Code |
InChI=1S/C9H18N2O3/c1-5(2)4-7(10)8(12)11-6(3)9(13)14/h5-7H,4,10H2,1-3H3,(H,11,12)(H,13,14)/t6-,7-/m0/s1
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| Chemical Name |
(2S)-2-[[(2S)-2-amino-4-methylpentanoyl]amino]propanoic acid
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| Synonyms |
Leu-ala; Alanine, N-L-leucyl-, L-; Leucyl-alanine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~494.44 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (494.44 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.9444 mL | 24.7219 mL | 49.4438 mL | |
| 5 mM | 0.9889 mL | 4.9444 mL | 9.8888 mL | |
| 10 mM | 0.4944 mL | 2.4722 mL | 4.9444 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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