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| Other Sizes |
| Targets |
Trypanothione Reductase, an enzyme involved in the antioxidant defense of trypanosomatid parasites. Leucogentian violet inhibits Trypanothione Reductase with an inhibition constant of 1.6 μM. Gentian violet is actively demethylated by liver microsomes and reduced to leucogentian violet by intestinal microflora.
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| ln Vitro |
Leucogentian violet inhibits Trypanothione Reductase with an inhibition constant of 1.6 μM. It is a triphenylmethane dye that can be used to detect antimony in environmental and biological samples using spectrophotometric techniques. The compound reacts with hydrogen peroxide and hemoglobin in blood to produce a purple/violet color.
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| ln Vivo |
In vivo activity data for leucogentian violet is limited as the compound is primarily used as a dye and analytical reagent. The compound's inhibition of Trypanothione Reductase suggests potential antiparasitic activity, but further studies are needed to confirm its in vivo efficacy. Gentian violet, the oxidized form, is reduced to leucogentian violet by intestinal microflora.
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| Enzyme Assay |
In vitro enzyme assays for leucogentian violet involve measuring the inhibition of Trypanothione Reductase activity. The enzyme is incubated with its substrate, trypanothione, and various concentrations of leucogentian violet. Enzyme activity is measured spectrophotometrically by monitoring the oxidation of NADPH or the reduction of DTNB. Inhibition constants (Ki = 1.6 μM) are determined from concentration-response curves.
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| Cell Assay |
In vitro cellular assays for leucogentian violet are limited as the compound is primarily used as a dye and analytical reagent. The compound may be used in cell staining applications due to its affinity for cellulosic and proteinaceous materials. Further cellular studies are needed to characterize its biological effects.
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| Animal Protocol |
In vivo animal studies for leucogentian violet are limited as the compound is primarily used as a dye and analytical reagent. Gentian violet, the oxidized form, has been studied in animal models, and its reduction to leucogentian violet by intestinal microflora has been documented. Further studies are needed to characterize the in vivo effects of leucogentian violet.
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| ADME/Pharmacokinetics |
Leucogentian violet has a molecular weight of 373.53 and a molecular formula of C25H31N3. It is soluble in water and should be stored in a brown glass bottle under refrigeration, protected from light and kept dry. The compound has a melting point of 175-177°C and a boiling point of 529.8°C.
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| Toxicity/Toxicokinetics |
The toxicological properties of leucogentian violet have been studied in the context of gentian violet metabolism. Gentian violet is actively demethylated by liver microsomes and reduced to leucogentian violet by intestinal microflora. The compound should be handled with appropriate safety precautions. Comprehensive toxicological evaluation is needed for any potential therapeutic applications.
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| References | |
| Additional Infomation |
White violet is a benzene-based aromatic compound.
Leucogentian violet (Leucocrystal Violet) is a triphenylmethane dye and the reduced, colorless form of gentian violet (crystal violet). It inhibits Trypanothione Reductase with an inhibition constant of 1.6 μM. The compound has an affinity for cellulosic and proteinaceous materials and reacts with hydrogen peroxide and hemoglobin to produce a purple/violet color. It is used as a dye and analytical reagent. |
| Molecular Formula |
C25H31N3
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|---|---|
| Molecular Weight |
373.54
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| Exact Mass |
373.251
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| CAS # |
603-48-5
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| Related CAS # |
Leucocrystal violet-d6;1173023-92-1
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| PubChem CID |
69048
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| Appearance |
Light green to green solid powder
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
529.8±50.0 °C at 760 mmHg
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| Melting Point |
175-177 °C(lit.)
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| Flash Point |
281.8±20.7 °C
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| Vapour Pressure |
0.0±1.4 mmHg at 25°C
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| Index of Refraction |
1.627
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| LogP |
6.02
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
28
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| Complexity |
370
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
OAZWDJGLIYNYMU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C25H31N3/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6/h7-18,25H,1-6H3
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| Chemical Name |
4-[bis[4-(dimethylamino)phenyl]methyl]-N,N-dimethylaniline
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| Synonyms |
C.I. Basic Violet 3, leuco; AI3-19978; Leucogentian violet
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~12.5 mg/mL (~33.46 mM)
H2O : ~0.67 mg/mL (~1.79 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 1.25 mg/mL (3.35 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6771 mL | 13.3854 mL | 26.7709 mL | |
| 5 mM | 0.5354 mL | 2.6771 mL | 5.3542 mL | |
| 10 mM | 0.2677 mL | 1.3385 mL | 2.6771 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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