| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
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| ln Vitro |
While untransformed lung cells are not inhibited by LCS3 (5 nM–10 µM; 96 hours), lung cancer cell lines are [1]. Sensitive lung adenocarcinoma cells (LUAD) are activated via the NRF2 pathway and ROS production by LCS3 (3 µM) at 3, 6, and 12 hours [1]. LCS3 (3 µM; 96 hours) selectively kills cell lines of lung adenocarcinoma (LUAD), partly through apoptosis induction [1].
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|---|---|
| Cell Assay |
Cell Viability Assay[1]
Cell Types: Non-small cell lung cancer (NSCLC) cells and non-transformed lung cells Tested Concentrations: 5 nM-10 µM Incubation Duration: 96 hrs (hours) Experimental Results: Inhibited growth of 24/25 NSCLC cell lines at low micromolar concentrations (IC50 <5μM), two non-transformed lung cell lines were relatively insensitive (IC50>10μM). Cell viability assay[1] Cell Types: H23 and H1650 Cell Tested Concentrations: 3 µM Incubation Duration: 3, 6 and 12 hrs (hours) Experimental Results: Respond to LCS3 by accumulating ROS and activating the NRF2 transcriptional program. Apoptosis analysis[1] Cell Types: Lung adenocarcinoma (LUAD) Cell Tested Concentrations: 3 µM Incubation Duration: 96 hrs (hours) Experimental Results: Increased cleavage of caspase 3, caspase 7 and/or PARP1 in all LCS3-sensitive LUAD cell lines. Western Blot Analysis[1] Cell Types: H23 and H1650 Cell Tested Concentrations: 3 µM Incubation Duration: 24 hrs (hours) Experimental Results: Protein levels of NRF2 and selected NRF2 downstream target products increased in both cell lines. |
| References |
| Molecular Formula |
C11H7N2O4CL
|
|---|---|
| Molecular Weight |
266.637
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| Exact Mass |
266.009
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| CAS # |
109844-92-0
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| PubChem CID |
844044
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| Appearance |
Light yellow to yellow solid powder
|
| LogP |
3.689
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| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
18
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| Complexity |
326
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
JDBZJNUHQINERI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H7ClN2O4/c12-7-1-3-8(4-2-7)13-11(15)9-5-6-10(18-9)14(16)17/h1-6H,(H,13,15)
|
| Chemical Name |
N-(4-chlorophenyl)-5-nitrofuran-2-carboxamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~468.80 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.38 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7504 mL | 18.7519 mL | 37.5038 mL | |
| 5 mM | 0.7501 mL | 3.7504 mL | 7.5008 mL | |
| 10 mM | 0.3750 mL | 1.8752 mL | 3.7504 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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