| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
|
||
| 50mg |
|
||
| 100mg | |||
| Other Sizes |
| Targets |
Laurdan does not target a specific protein or receptor. Its mechanism of action is based on its photophysical properties. Upon excitation with UV light, Laurdan emits fluorescence, and the emission maximum shifts from ~490 nm in a nonpolar environment to ~440 nm in a polar environment. This shift is due to the relaxation of the excited state dipole moment in response to the surrounding solvent molecules. In membranes, this property allows the quantification of membrane polarity and hydration, which are indicators of lipid packing and phase state.
|
|---|---|
| ln Vitro |
In vitro, Laurdan is used to characterize the physical properties of model membranes (liposomes) and biological membranes. Its fluorescence emission spectrum is recorded, and the generalized polarization (GP) value is calculated. The GP value is a quantitative measure of membrane polarity and phase state. It is used to study the effects of temperature, lipid composition, cholesterol, and proteins on membrane properties.
|
| ln Vivo |
In vivo, Laurdan is not used as a drug. It is used as a research tool in cell biology. It can be incorporated into the plasma membranes of live cells to study membrane dynamics and the effects of drugs or environmental changes on membrane properties. It is used in studies of cellular processes such as membrane trafficking, signaling, and cell stress.
|
| Enzyme Assay |
Non-cell-based assays for Laurdan involve incorporating the probe into liposomes or isolated membranes. The fluorescence emission spectrum is recorded using a spectrofluorometer, and the GP value is calculated from the intensities at 440 nm and 490 nm. This provides a quantitative measure of membrane polarity.
|
| Cell Assay |
Cellular assays for Laurdan involve staining live cells with the probe. The cells are then analyzed using fluorescence microscopy or flow cytometry. The GP value is calculated for individual cells or regions of interest, allowing for the study of membrane heterogeneity and dynamics in a cellular context.
|
| Animal Protocol |
In vivo animal models for Laurdan are not typical, as it is a fluorescent probe used primarily in in vitro and cell-based studies. However, it can be used in ex vivo studies on isolated tissues to study membrane properties.
|
| ADME/Pharmacokinetics |
Laurdan has a molecular weight of 353.54 g/mol and a molecular formula of C₂₄H₃₅NO₂. Its CAS number is 74515-25-6. The compound is a solid. Purity is typically ≥98%. It is soluble in DMSO. Storage conditions: -20°C, protected from light. The compound is supplied for research use only.
|
| Toxicity/Toxicokinetics |
Laurdan is a fluorescent probe with low toxicity at typical staining concentrations. The compound is intended for research use only and is not for human consumption. Standard laboratory safety precautions should be followed.
|
| Additional Infomation |
Laurdan is a solvatochromic fluorescent probe used to study membrane polarity and phase state. It is a valuable tool for membrane biophysics and cell biology research. Its CAS number is 74515-25-6.
|
| Molecular Formula |
C24H35NO
|
|---|---|
| Molecular Weight |
353.5408
|
| Exact Mass |
353.272
|
| CAS # |
74515-25-6
|
| PubChem CID |
104983
|
| Appearance |
White to yellow solid powder
|
| Density |
0.987g/cm3
|
| Boiling Point |
497.4ºC at 760 mmHg
|
| Melting Point |
88ºC(lit.)
|
| Flash Point |
177.4ºC
|
| Index of Refraction |
1.553
|
| LogP |
7.009
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
12
|
| Heavy Atom Count |
26
|
| Complexity |
392
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
JHDGGIDITFLRJY-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C24H35NO/c1-4-5-6-7-8-9-10-11-12-13-24(26)22-15-14-21-19-23(25(2)3)17-16-20(21)18-22/h14-19H,4-13H2,1-3H3
|
| Chemical Name |
1-[6-(dimethylamino)naphthalen-2-yl]dodecan-1-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~12.5 mg/mL (~35.36 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (3.54 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8285 mL | 14.1427 mL | 28.2853 mL | |
| 5 mM | 0.5657 mL | 2.8285 mL | 5.6571 mL | |
| 10 mM | 0.2829 mL | 1.4143 mL | 2.8285 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
